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AB270278

Anti-Connexin 32 / GJB1 antibody [M12.13] - BSA and Azide free

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Mouse Monoclonal Connexin 32 / GJB1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Native Full Length Protein corresponding to Rat Gjb1.

View Alternative Names

Cxn-32, Gap junction beta-1 protein, Connexin-32, GAP junction 28 kDa liver protein, Cx32

1 Images
Western blot - Anti-Connexin 32 / GJB1 antibody [M12.13] - BSA and Azide free (AB270278)
  • WB

Supplier Data

Western blot - Anti-Connexin 32 / GJB1 antibody [M12.13] - BSA and Azide free (AB270278)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270241).

All lanes:

Western blot - Anti-Connexin 32 / GJB1 antibody [M12.13] - BSA and Azide free (ab270278) at 2 µg/mL

All lanes:

Human stomach lysate

Predicted band size: 32 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

M12.13

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Native Full Length Protein corresponding to Rat Gjb1.

P08033

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Reactivity data

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Product details

ab270278 is a carrier free version of ab270241.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from bioreactor concentrate.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Connexin 32 also known as GJB1 is a gap junction protein with a molecular mass of approximately 32 kDa. This protein facilitates direct intercellular communication by forming gap junction channels that allow ions and small molecules to pass between adjacent cells. Connexin 32 is expressed in various tissues including liver pancreas and many types of epithelial cells. Its expression ensures cells can synchronize their activities important for maintaining tissue homeostasis.
Biological function summary

Connexin 32 plays a significant role in maintaining cellular communication networks. This protein forms hexameric complexes called connexons which dock with connexons of adjacent cells to create functional gap junction channels. These channels are essential in processes such as maintaining homeostasis cell differentiation and synchronized contraction of tissues like cardiac muscle. The structural formation allows direct transfer of metabolites and signaling molecules highlighting its importance in multicellular organism function.

Pathways

Connexin 32 actively contributes to cellular communication and metabolic coordination. It participates in the phosphoinositide 3-kinase (PI3-K) pathway and the ERK/MAPK signaling pathway which are important for cell growth proliferation and apoptosis. Connexin 32 interacts with proteins like Connexin 43 with both proteins playing roles in channel assembly and intracellular signaling processes that influence cellular responses to external stimuli.

Connexin 32's dysfunction aligns with specific neuropathies. Mutations in the GJB1 gene often lead to X-linked Charcot-Marie-Tooth disease a disorder affecting peripheral nerves. This disease involves degeneration of nerve fibers disrupting muscle function and sensation and connects with proteins that share functional pathways like PMP22. Additionally Connexin 32 has implications in liver diseases where its reduced expression correlates with disrupted hepatocyte communication affecting liver function and regeneration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
See full target information Gjb1
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Product promise

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