AB230537

Anti-Connexin 43 / GJA1 antibody

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(6 Publications)

Rabbit Polyclonal Connexin 43 / GJA1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human GJA1 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

GJAL, GJA1, Gap junction alpha-1 protein, Connexin-43, Gap junction 43 kDa heart protein, Cx43

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Connexin 43 / GJA1 antibody (AB230537)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Connexin 43 / GJA1 antibody (AB230537)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Connexin 43 / GJA1 using ab230537 at 1/100 dilution in ICC/IF.

Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight^® 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody (AB230537)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody (AB230537)

Formalin-fixed, paraffin-embedded human heart tissue stained for Connexin 43 / GJA1 using ab230537 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Connexin 43 / GJA1 antibody (AB230537)

All lanes:

Western blot - Anti-Connexin 43 / GJA1 antibody (ab230537) at 1/500 dilution

Lane 1:

C6 (rat glial tumor cell line) whole cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 3:

C2C12 (mouse myoblast cell line) whole cell lysate

Lane 4:

Mouse heart tissue lysate

Lane 5:

Rat brain tissue lysate

Predicted band size: 43 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human GJA1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P17302

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Connexin 43 also known as GJA1 is a protein that plays an important role in cell communication through gap junctions. It has a molecular weight of approximately 43 kDa. Connexin 43 gets expressed in various tissues including the heart and brain where it facilitates cellular signaling. This protein integrates into cell membranes to form gap junction channels allowing direct transfer of ions and small molecules between adjacent cells. Researchers often use techniques such as Western blot to detect Connexin 43 expression levels in studies of cellular communications.

Biological function summary

This protein facilitates electrical and chemical coupling between cells by forming intercellular channels. These channels arise from the assembly of six Connexin 43 subunits into hemichannels which dock with hemichannels on neighboring cells. This protein has a critical component in the cardiac and neural tissues where rapid communication is essential for proper function. Connexin 43 engages in complex assemblies with other connexins enhancing its role in synchronizing cell activities.

Pathways

Connexin 43 significantly influences signal transduction processes. It acts within the MAPK signaling pathway which impacts cellular responses such as growth and differentiation. Additionally this protein plays a role in calcium signaling a pathway important for muscle contraction and neurotransmitter release. Interacting proteins like Connexin 30 and Connexin 45 further regulate these pathways affecting the amplitude and duration of signaling.

Connexin 43 has been linked to cardiac arrhythmias and some forms of cancer. Mutations or altered expression of Connexin 43 can disrupt the electrical conduction in cardiac tissue leading to arrhythmogenic conditions. Research suggests an association with proteins like ZO-1 and Connexin 37 which may affect cell proliferation and migration in cancerous tissues. Understanding these connections highlights Connexin 43's potential as a target for therapeutic interventions in related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity : acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity).

See full target information GJA1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:124 PubMed40055817

2025

Extracellular vesicular delivery of ceramides from pulmonary macrophages to endothelial cells facilitates chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Qiqing Huang,Tutu Kang,Shaoran Shen,Lele Liu,Lili Zhang,Xiaoli Zou,Jianqing Wu

Renal failure 46:2398710 PubMed39238246

2024

Chrysophanol-mediated trx-1 activation attenuates renal fibrosis through inhibition of the JNK/Cx43 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Neng Bao,Jin Wang,Qiyu Yue,Fang Cao,Xuejing Gu,Kejian Wen,Wei Kong,Mingjia Gu

Cell reports 42:111988 PubMed36640364

2023

Astrocytic cell adhesion genes linked to schizophrenia correlate with synaptic programs in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Olli Pietiläinen,Aditi Trehan,Daniel Meyer,Jana Mitchell,Matthew Tegtmeyer,Vera Valakh,Hilena Gebre,Theresa Chen,Emilia Vartiainen,Samouil L Farhi,Kevin Eggan,Steven A McCarroll,Ralda Nehme

QJM : monthly journal of the Association of Physicians 116:197-204 PubMed36200913

2022

Apelin as a new therapeutic target for COVID-19 treatment.

Applications

Unspecified application

Species

Unspecified reactive species

J Park,M-Y Park,Y Kim,Y Jun,U Lee,C-M Oh

Cardiovascular therapeutics 2022:9966306 PubMed35360546

2022

Upregulated miR-206 Aggravates Deep Vein Thrombosis by Regulating GJA1-Mediated Autophagy of Endothelial Progenitor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Li,Jingping Ge,Yuanyuan Yin,Ruowen Yang,Jie Kong,Jianping Gu

Frontiers in cell and developmental biology 9:637233 PubMed34169070

2021

Propofol Mediated Protection of the Brain From Ischemia/Reperfusion Injury Through the Regulation of Microglial Connexin 43.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Zhang,Yanyan Wang,Qin Xia,Zhiyi Tu,Jiajun Sun,Qi Jing,Pei Chen,Xuan Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Connexin 43 / GJA1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Connexin 43 / GJA1 antibody (AB230537)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody (AB230537)

Western blot - Anti-Connexin 43 / GJA1 antibody (AB230537)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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