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Rabbit Recombinant Monoclonal Connexin 43 / GJA1 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 5 publications.


Images

Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] (AB217676), expandable thumbnail
  • Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] (AB217676), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR21153] (AB217676), expandable thumbnail
  • Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR21153] (AB217676), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR21153] (AB217676), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/30

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity).

Alternative names

Gap junction alpha-1 protein, Connexin-43, Gap junction 43 kDa heart protein, Cx43, GJA1, GJAL

Recommended products

Rabbit Recombinant Monoclonal Connexin 43 / GJA1 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 5 publications.

Alternative names

Gap junction alpha-1 protein, Connexin-43, Gap junction 43 kDa heart protein, Cx43, GJA1, GJAL

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR21153

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Connexin 43 also known as GJA1 is a protein that plays an important role in cell communication through gap junctions. It has a molecular weight of approximately 43 kDa. Connexin 43 gets expressed in various tissues including the heart and brain where it facilitates cellular signaling. This protein integrates into cell membranes to form gap junction channels allowing direct transfer of ions and small molecules between adjacent cells. Researchers often use techniques such as Western blot to detect Connexin 43 expression levels in studies of cellular communications.

Biological function summary

This protein facilitates electrical and chemical coupling between cells by forming intercellular channels. These channels arise from the assembly of six Connexin 43 subunits into hemichannels which dock with hemichannels on neighboring cells. This protein has a critical component in the cardiac and neural tissues where rapid communication is essential for proper function. Connexin 43 engages in complex assemblies with other connexins enhancing its role in synchronizing cell activities.

Pathways

Connexin 43 significantly influences signal transduction processes. It acts within the MAPK signaling pathway which impacts cellular responses such as growth and differentiation. Additionally this protein plays a role in calcium signaling a pathway important for muscle contraction and neurotransmitter release. Interacting proteins like Connexin 30 and Connexin 45 further regulate these pathways affecting the amplitude and duration of signaling.

Associated diseases and disorders

Connexin 43 has been linked to cardiac arrhythmias and some forms of cancer. Mutations or altered expression of Connexin 43 can disrupt the electrical conduction in cardiac tissue leading to arrhythmogenic conditions. Research suggests an association with proteins like ZO-1 and Connexin 37 which may affect cell proliferation and migration in cancerous tissues. Understanding these connections highlights Connexin 43's potential as a target for therapeutic interventions in related diseases.

Product promise

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Full details and terms and conditions can be found here:
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7 product images

  • Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676), expandable thumbnail

    Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676)

    Lanes 1 - 4: Merged signal (red and green). Green - ab217676 observed at 43 kDa. Red - loading control, Anti-Vinculin antibody [VIN-54] ab130007, observed at 130 kDa.

    ab217676 was shown to recognize Connexin 43 / GJA1 in wild-type HEK 293 cells as signal was lost at the expected MW in GJA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GJA1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab217676 and Anti-Vinculin antibody [VIN-54] ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676) at 1/1000 dilution

    Lane 1: Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Lane 2: GJA1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 43 kDa

  • Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676), expandable thumbnail

    Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.
    Lane 1: 37 seconds.
    Lane 2: 15 seconds.
    Lane 3: 103 seconds.
    Lane 4: 3 minutes.

    The expression profile is consistent with the existence of natural variants described in the literature (PMID: 24210816; PMID: 28576298).

    All lanes: Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676) at 1/1000 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: Human testis tissue lysate at 20 µg

    Lane 3: Human fetal brain tissue lysate at 20 µg

    Lane 4: Human breast cancer tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa

    Observed band size: 26-32 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676)

    Immunohistochemical analysis of paraffin-embedded human prostate tissue labeling Connexin 43 / GJA1 with ab217676 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use. Positive staining on basal cells of human prostate gland (PMID: 20735413) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use.

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676), expandable thumbnail

    Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676)

    Connexin 43 / GJA1 was immunoprecipitated from 0.35 mg of human testis lysate with ab217676 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab217676 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Human testis lysate 10 μg (Input).
    Lane 2: ab217676 IP in human testis lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab217676 in human testis lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 minute.

    The expression profile is consistent with the existence of natural variants described in the literature (PMID: 24210816; PMID: 28576298).

    All lanes: Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676)

    Predicted band size: 43 kDa

    Observed band size: 26 kDa, 43 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Connexin 43 / GJA1 with ab217676 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use. Positive staining on human cardiac muscle (PMID: 25018732) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use.

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676), expandable thumbnail

    Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18649180; PMID: 30448479).
    This blot was developed using a high sensitivity ECL substrate.
    Alkaline phosphatase. -: untreated membrane; +: phosphatase treated membrane.
    In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
    Anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676) total protein control staining at 1/1000 dilution.
    Exposure time: 92 seconds

    All lanes: Western blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] ab312836) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) cells transfected with an empty vector containi a myc-His-tag® whole cell lysate at 20 µg

    Lane 2: HeLa cells transfected with a human GJA1 expression vector containi a myc-His-tag® whole cell lysate at 20 µg

    Lane 3: HeLa cells transfected with a human GJA1(Mutant Y265F) expression vector containi a myc-His-tag® whole cell lysate at 20 µg

    Lane 4: HeLa cells transfected with a RSV-SR-A V-SRC expression vector containi a myc-His-tag® whole cell lysate at 20 µg

    Lane 5: HeLa cells co-transfected with a human GJA1 expression vector containi a myc-His-tag® and a RSV-SR-A V-SRC expression vector containi a myc-His-tag® whole cell lysate at 20 µg

    Lane 6: HeLa cells co-transfected with a human GJA1(Mutant Y265F) expression vector containi a myc-His-tag® and a RSV-SR-A V-SRC expression vector containi a myc-His-tag® whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 43 kDa

    Exposure time: 92s

  • Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676), expandable thumbnail

    Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676)

    Western blot: Anti-GJA1 antibody [EPR21153] (ab217676) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab217676 was shown to bind specifically to GJA1. A band was observed at 45 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in GJA1 knockout cell line. To generate this image, wild-type and GJA1 knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] (ab217676) at 1/1000 dilution

    Lane 1: Wild-type U-87 MG cell lysate at 20 µg

    Lane 3: Wild-type HEK-293 cell lysate at 20 µg

    Lane 4: GJA1 knockout HEK-293 Human GJA1 knockout HEK-293 cell lysate ab261658 cell lysate at 20 µg

    Secondary

    Lanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

    Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 45 kDa

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