Rabbit Recombinant Monoclonal Connexin 43 / GJA1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.
Images
![Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (AB235625), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr21153-bsa-and-azide-free-ab235625--western-blot-img180473.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (AB235625)")
![Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (AB235625), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr21153-bsa-and-azide-free-ab235625--western-blot-img179683.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (AB235625)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (AB235625), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr21153-bsa-and-azide-free-ab235625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109162.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (AB235625)")
![Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (AB235625), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr21153-bsa-and-azide-free-ab235625--immunoprecipitation-img3990.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (AB235625)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (AB235625), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr21153-bsa-and-azide-free-ab235625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117632.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (AB235625)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
10 products for Alternative Product
Target data
Function
Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity).
Alternative names
GJAL, GJA1, Gap junction alpha-1 protein, Connexin-43, Gap junction 43 kDa heart protein, Cx43
Recommended products
Rabbit Recombinant Monoclonal Connexin 43 / GJA1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR21153
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab235625 is the carrier-free version of Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Connexin 43 also known as GJA1 is a protein that plays an important role in cell communication through gap junctions. It has a molecular weight of approximately 43 kDa. Connexin 43 gets expressed in various tissues including the heart and brain where it facilitates cellular signaling. This protein integrates into cell membranes to form gap junction channels allowing direct transfer of ions and small molecules between adjacent cells. Researchers often use techniques such as Western blot to detect Connexin 43 expression levels in studies of cellular communications.
- Biological function summary
This protein facilitates electrical and chemical coupling between cells by forming intercellular channels. These channels arise from the assembly of six Connexin 43 subunits into hemichannels which dock with hemichannels on neighboring cells. This protein has a critical component in the cardiac and neural tissues where rapid communication is essential for proper function. Connexin 43 engages in complex assemblies with other connexins enhancing its role in synchronizing cell activities.
- Pathways
Connexin 43 significantly influences signal transduction processes. It acts within the MAPK signaling pathway which impacts cellular responses such as growth and differentiation. Additionally this protein plays a role in calcium signaling a pathway important for muscle contraction and neurotransmitter release. Interacting proteins like Connexin 30 and Connexin 45 further regulate these pathways affecting the amplitude and duration of signaling.
- Associated diseases and disorders
Connexin 43 has been linked to cardiac arrhythmias and some forms of cancer. Mutations or altered expression of Connexin 43 can disrupt the electrical conduction in cardiac tissue leading to arrhythmogenic conditions. Research suggests an association with proteins like ZO-1 and Connexin 37 which may affect cell proliferation and migration in cancerous tissues. Understanding these connections highlights Connexin 43's potential as a target for therapeutic interventions in related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr21153-bsa-and-azide-free-ab235625--western-blot-img180473.jpg "Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625)")
Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676).
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lane 1: 37 seconds.
Lane 2: 15 seconds.
Lane 3: 103 seconds.
Lane 4: 3 minutes.The expression profile is consistent with the existence of natural variants described in the literature (PMID: 24210816; PMID: 28576298).
All lanes: Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625) at 1/1000 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: Human testis tissue lysate at 20 µg
Lane 3: Human fetal brain tissue lysate at 20 µg
Lane 4: Human breast cancer tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 20-32 kDa
![Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr21153-bsa-and-azide-free-ab235625--western-blot-img179683.jpg "Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625)")
Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676 observed at 43 kDa. Red - loading control, Anti-Vinculin antibody [VIN-54] ab130007, observed at 130 kDa.
Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676 was shown to recognize Connexin 43 / GJA1 in wild-type HEK 293 cells as signal was lost at the expected MW in GJA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GJA1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676 and Anti-Vinculin antibody [VIN-54] ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625) at 1/1000 dilution
Lane 1: Wild-type HEK 293 whole cell lysate at 20 µg
Lane 2: GJA1 knockout HEK 293 whole cell lysate at 20 µg
Lane 3: Hela whole cell lysate at 20 µg
Predicted band size: 43 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr21153-bsa-and-azide-free-ab235625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109162.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625)
Connexin 43 / GJA1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Connexin 43 / GJA1 antibody
Immunohistochemical analysis of paraffin-embedded human prostate tissue labeling Connexin 43 / GJA1 with Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use. Positive staining on basal cells of human prostate gland (PMID: 20735413) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676).
![Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr21153-bsa-and-azide-free-ab235625--immunoprecipitation-img3990.jpg "Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625)")
Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625)
Connexin 43 / GJA1 was immunoprecipitated from 0.35 mg of human testis lysate with Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Lane 1: Human testis lysate 10 μg (Input).
Lane 2: Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676 IP in human testis lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676 in human testis lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 minute.The expression profile is consistent with the existence of natural variants described in the literature (PMID: 24210816; PMID: 28576298).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676).
All lanes: Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker (Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676)
Predicted band size: 43 kDa
Observed band size: 26 kDa, 43 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr21153-bsa-and-azide-free-ab235625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117632.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625)
Connexin 43 / GJA1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Connexin 43 / GJA1 antibody
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Connexin 43 / GJA1 with Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use. Positive staining on human cardiac muscle (PMID: 25018732) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676).
![Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr21153-bsa-and-azide-free-ab235625--western-blot-img438848.jpg "Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625)")
Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625)
Connexin 43 / GJA1 Western blot staining using rabbit Anti-Connexin 43 / GJA1 antibody
This data was developed using Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676, the same antibody clone in a different buffer formulation.
Western blot: Anti-GJA1 antibody [EPR21153] (Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676 was shown to bind specifically to GJA1. A band was observed at 45 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in GJA1 knockout cell line. To generate this image, wild-type and GJA1 knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker (Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676) at 1/1000 dilution
Lane 1: Wild-type U-87 MG cell lysate at 20 µg
Lane 2: Western blot - Human GJA1 knockout U-87 MG cell line (ab306676)
Lane 3: Wild-type HEK-293 cell lysate at 20 µg
Lane 4: GJA1 knockout HEK-293 Human GJA1 knockout HEK-293 cell lysate ab261658 cell lysate at 20 µg
SecondaryLanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution
Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 45 kDa
![Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr21153-bsa-and-azide-free-ab235625--western-blot-img438848.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Connexin 43 / GJA1 antibody [EPR21153] - BSA and Azide free (ab235625)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com