Rabbit Recombinant Monoclonal Connexin 43 / GJA1 antibody. Carrier free. Suitable for IHC-Fr, IP, WB, IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (AB259276), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr22955-101-bsa-and-azide-free-ab259276--immunoprecipitation-img3886.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (AB259276)")
![Western blot - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (AB259276), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr22955-101-bsa-and-azide-free-ab259276--western-blot-img9209.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (AB259276)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (AB259276), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr22955-101-bsa-and-azide-free-ab259276--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49442.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (AB259276)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (AB259276), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr22955-101-bsa-and-azide-free-ab259276--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img46443.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (AB259276)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (AB259276), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr22955-101-bsa-and-azide-free-ab259276--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130386.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (AB259276)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-Fr | IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Expected | Expected | Not recommended | Tested | Not recommended | Tested |
| Mouse | Tested | Tested | Not recommended | Tested | Not recommended | Tested |
| Rat | Tested | Expected | Not recommended | Tested | Not recommended | Tested |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity).
Alternative names
GJAL, GJA1, Gap junction alpha-1 protein, Connexin-43, Gap junction 43 kDa heart protein, Cx43
Recommended products
Rabbit Recombinant Monoclonal Connexin 43 / GJA1 antibody. Carrier free. Suitable for IHC-Fr, IP, WB, IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22955-101
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody shows low affinity in detecting cell line samples.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab259276 is the carrier-free version of Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Connexin 43 also known as GJA1 is a protein that plays an important role in cell communication through gap junctions. It has a molecular weight of approximately 43 kDa. Connexin 43 gets expressed in various tissues including the heart and brain where it facilitates cellular signaling. This protein integrates into cell membranes to form gap junction channels allowing direct transfer of ions and small molecules between adjacent cells. Researchers often use techniques such as Western blot to detect Connexin 43 expression levels in studies of cellular communications.
- Biological function summary
This protein facilitates electrical and chemical coupling between cells by forming intercellular channels. These channels arise from the assembly of six Connexin 43 subunits into hemichannels which dock with hemichannels on neighboring cells. This protein has a critical component in the cardiac and neural tissues where rapid communication is essential for proper function. Connexin 43 engages in complex assemblies with other connexins enhancing its role in synchronizing cell activities.
- Pathways
Connexin 43 significantly influences signal transduction processes. It acts within the MAPK signaling pathway which impacts cellular responses such as growth and differentiation. Additionally this protein plays a role in calcium signaling a pathway important for muscle contraction and neurotransmitter release. Interacting proteins like Connexin 30 and Connexin 45 further regulate these pathways affecting the amplitude and duration of signaling.
- Associated diseases and disorders
Connexin 43 has been linked to cardiac arrhythmias and some forms of cancer. Mutations or altered expression of Connexin 43 can disrupt the electrical conduction in cardiac tissue leading to arrhythmogenic conditions. Research suggests an association with proteins like ZO-1 and Connexin 37 which may affect cell proliferation and migration in cancerous tissues. Understanding these connections highlights Connexin 43's potential as a target for therapeutic interventions in related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr22955-101-bsa-and-azide-free-ab259276--immunoprecipitation-img3886.jpg "Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)")
Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)
Connexin 43 / GJA1 was immunoprecipitated from 0.35 mg mouse brain lysate 10μg with Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain lysate 10μg.
Lane 2: Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585 IP in mouse brain lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585 in mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
High MW band between 75kDa and 100kDa represents Connexin 43 dimers which described in literature. (PMID: 19460776).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585).
All lanes: Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585)
Predicted band size: 43 kDa
Observed band size: 43 kDa
![Western blot - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr22955-101-bsa-and-azide-free-ab259276--western-blot-img9209.jpg "Western blot - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)")
Western blot - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585 has low affinity in cell line samples.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker (Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585) at 1/1000 dilution
Lane 1: Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: C2C12 (Mouse myoblasts myoblast) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 43 kDa
Exposure time: 180s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr22955-101-bsa-and-azide-free-ab259276--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49442.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Connexin 43 / GJA1 with Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585 at 1/1000 dilution (0.507 μg/ml) followed by a ready to use Goat Anti-Rabbit & Mouse IgG (HRP). Positive staining on rat myocardial intercalated disc is observed. The section was incubated with Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit & Mouse IgG (HRP).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr22955-101-bsa-and-azide-free-ab259276--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img46443.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Connexin 43 / GJA1 with Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585 at 1/1000 dilution (0.507 μg/ml) followed by a ready to use Goat Anti-Rabbit & Mouse IgG (HRP). Membranous staining on mouse testis is observed. The section was incubated with Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit & Mouse IgG (HRP).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr22955-101-bsa-and-azide-free-ab259276--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130386.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Connexin 43 / GJA1 with Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585 at 1/1000 dilution (0.507 μg/ml) followed by a ready to use Goat Anti-Rabbit & Mouse IgG (HRP). Positive staining on mouse myocardial intercalated disc (PMID: 30228305) is observed. The section was incubated with Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit & Mouse IgG (HRP).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr22955-101-bsa-and-azide-free-ab259276--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120664.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Connexin 43 / GJA1 with Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585 at 1/500 dilution (1.01 ug/ml) followed by a ready to use Goat Anti-Rabbit & Mouse IgG (HRP). Positive staining on human myocardial intercalated disc. The section was incubated with Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit & Mouse IgG (HRP).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585).
![Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr22955-101-bsa-and-azide-free-ab259276--immunohistochemistry-frozen-sections-img120104.jpg "Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)")
Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)
4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of rat cardiac muscle tissue labeling Connexin 43 / GJA1 using Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). The nuclear counterstain is DAPI (blue). Positive staining on intercalated discs of rat cardiac muscle (PMID: 18678643) is observed.
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585).
![Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr22955-101-bsa-and-azide-free-ab259276--immunohistochemistry-frozen-sections-img124835.jpg "Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)")
Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)
4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of mouse cardiac muscle tissue labeling Connexin 43 / GJA1 using Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). The nuclear counterstain is DAPI (blue). Positive staining on intercalated discs of mouse cardiac muscle (PMID: 18678643) is observed.
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr22955-101-bsa-and-azide-free-ab259276--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120664.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)")
![Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr22955-101-bsa-and-azide-free-ab259276--immunohistochemistry-frozen-sections-img120104.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)")
![Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276), expandable thumbnail](https://content.abcam.com/products/images/connexin-43-gja1-antibody-epr22955-101-bsa-and-azide-free-ab259276--immunohistochemistry-frozen-sections-img124835.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - BSA and Azide free (ab259276)")
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