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Rabbit Polyclonal Connexin 43 / GJA1 antibody. Intercellular Junction marker. Suitable for ICC, WB, IHC-P, IHC-Fr and reacts with Hamster, Mouse, Rat, Human, Dog, Cow, Pig, Monkey samples. Cited in 309 publications. Immunogen corresponding to Synthetic Peptide within Human GJA1 aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 1% BSA, 0.0268% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

Synthetic Peptide within Human GJA1 aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link: P17302

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCWBIHC-PIHC-Fr
Human
Expected
Tested
Tested
Expected
Mouse
Expected
Tested
Tested
Expected
Rat
Expected
Tested
Expected
Expected
Cow
Expected
Expected
Tested
Expected
Dog
Expected
Tested
Expected
Expected
Hamster
Tested
Expected
Expected
Expected
Monkey
Predicted
Predicted
Predicted
Expected
Pig
Predicted
Predicted
Predicted
Expected

Images

Tested
Tested

Species
Hamster
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, Human, Dog, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Monkey, Pig
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000.00000 - 1/8000.00000
Notes

-

Species
Rat
Dilution info
1/2000.00000 - 1/8000.00000
Notes

-

Species
Human
Dilution info
1/2000.00000 - 1/8000.00000
Notes

-

Species
Dog
Dilution info
1/2000.00000 - 1/8000.00000
Notes

-

Expected
Expected

Species
Hamster, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Monkey, Pig
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000.00000 - 1/5000.00000
Notes

-

Species
Human
Dilution info
1/1000.00000 - 1/5000.00000
Notes

-

Species
Cow
Dilution info
1/1000.00000 - 1/5000.00000
Notes

-

Expected
Expected

Species
Hamster, Rat, Dog
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Monkey, Pig
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, Pig, Hamster, Monkey, Human, Dog, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

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Rabbit Polyclonal Connexin 43 / GJA1 antibody. Intercellular Junction marker. Suitable for ICC, WB, IHC-P, IHC-Fr and reacts with Hamster, Mouse, Rat, Human, Dog, Cow, Pig, Monkey samples. Cited in 309 publications. Immunogen corresponding to Synthetic Peptide within Human GJA1 aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen

Synthetic Peptide within Human GJA1 aa 350 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link: P17302

Purification technique

Affinity purification Immunogen

Concentration
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Purification notes

Affinity isolated antigen specific antibody is obtained by immunospecific purification which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to connexin 43.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Storage in frost-free freezers is not recommended. 
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077). Or search our wide range of secondary antibodies for use with your experiment.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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