AB235282

Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker

Name: Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab235282) | Abcam
Brand: Abcam Limited
SKU: AB235282
Price: 525 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal Connexin 43 / GJA1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

Cxn-43, Gja1, Gap junction alpha-1 protein, Connexin-43, Gap junction 43 kDa heart protein, Cx43

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (AB235282)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (AB235282)

Immunohistochemical analysis of paraffin-embedded mouse heart tissue labeling Connexin 43 - GJA1 with ab235282 at 1/200 dilution (30 minutes at RT), followed by a ready to use anti-Rabbit polyclonal IgG (HRP) (8 minutes at RT). Primary staining is DAB. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval using citrate buffer for 20 minutes.

Panel A : Mouse heart H&E.
Panel B : ab235282 at 20X magnification.
Panel C : ab235282 at 40X magnification.

Immunocytochemistry/ Immunofluorescence - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (AB235282)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (AB235282)

Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized rat pup brain cells labeling Connexin 43 / GJA1 with ab235282 at 15 μg/ml (Overnight at 4°C), followed by a Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 5 μg/ml (1 hour at RT) (green). The nuclear counterstain is DAPI (blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (AB235282)

Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling Connexin 43 / GJA1 with ab235282 at 15 μg/ml (Overnight at 4°C), followed by a Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 5 μg/ml (1 hour at RT) (green). The nuclear counterstain is DAPI (blue).

Supplier Data

Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (AB235282)

Exposure time : 0.5 secs.

All lanes:

Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab235282) at 1/1000 dilution

Lane 1:

Mouse heart tissue at 35 µg

Lane 2:

Rat brain tissue at 35 µg

Secondary

All lanes:

Goat anti-rabbit-HRP at 1/70000 dilution

Predicted band size: 43 kDa

Observed band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "15 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "15 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Connexin 43 also known as GJA1 is a protein that plays an important role in cell communication through gap junctions. It has a molecular weight of approximately 43 kDa. Connexin 43 gets expressed in various tissues including the heart and brain where it facilitates cellular signaling. This protein integrates into cell membranes to form gap junction channels allowing direct transfer of ions and small molecules between adjacent cells. Researchers often use techniques such as Western blot to detect Connexin 43 expression levels in studies of cellular communications.

Biological function summary

This protein facilitates electrical and chemical coupling between cells by forming intercellular channels. These channels arise from the assembly of six Connexin 43 subunits into hemichannels which dock with hemichannels on neighboring cells. This protein has a critical component in the cardiac and neural tissues where rapid communication is essential for proper function. Connexin 43 engages in complex assemblies with other connexins enhancing its role in synchronizing cell activities.

Pathways

Connexin 43 significantly influences signal transduction processes. It acts within the MAPK signaling pathway which impacts cellular responses such as growth and differentiation. Additionally this protein plays a role in calcium signaling a pathway important for muscle contraction and neurotransmitter release. Interacting proteins like Connexin 30 and Connexin 45 further regulate these pathways affecting the amplitude and duration of signaling.

Connexin 43 has been linked to cardiac arrhythmias and some forms of cancer. Mutations or altered expression of Connexin 43 can disrupt the electrical conduction in cardiac tissue leading to arrhythmogenic conditions. Research suggests an association with proteins like ZO-1 and Connexin 37 which may affect cell proliferation and migration in cancerous tissues. Understanding these connections highlights Connexin 43's potential as a target for therapeutic interventions in related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Structural component of the gap junction, a specialized intercellular structure consisting of a cluster of closely packed pairs of transmembrane channels, the connexons, that allow passage of small molecules and electrical signals between neighboring cells. Forms homotypic and heterotypic channels gated by transjunctional voltage (By similarity). May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph (By similarity). Negative regulator of bladder functional capacity : acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in the conductive system of ventricular myocardium and heart morphogenesis (PubMed : 26403541). May play a role in cell growth inhibition through the regulation of NOV expression and localization (PubMed : 15181016). Involved in intercellular innate immune signaling. Mediates translocation of 2',3'-cGAMP and 2',5'-oligoadenylates (2-5A) second messengers from virus-infected cells to macrophages and uninfected neighboring cells to propagate and amplify the antiviral immune response (By similarity).. Connexin 43 is possibly the ATP-induced pore of mouse macrophages.

See full target information Gja1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:575 PubMed40830790

2025

Engineered MEVs for photoreceptor-targeted delivery of USP25 to alleviate diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoxiang Sun,Shenyuan Chen,Xiaoyuan Qi,Yuntong Sun,Zhengmei Jiang,Jie Chang,Yongjun Ma,Jin Huang,Benshuai You,Fengtian Sun

PloS one 20:e0322721 PubMed40694571

2025

Busulfan damages spermatogenic function by inducing orchitis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingjun Zhao,Kaihui Wu,Shiyuan Xu,Songqi Liu,Kaimin Yuan,Dong Wang

International journal of molecular medicine 54: PubMed39129277

2024

Unveiling the role of RhoA and ferroptosis in vascular permeability: Implications for osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxin He,Kuanmin Tian,Xue Lin,Xiaolei Chen,Yajing Su,Zhidong Lu,Zhirong Chen,Liang Zhang,Peng Li,Long Ma,Gangning Feng,Xin Zhao,Zhibin Lan,Chen Zhang,Di Xue,Qunhua Jin

Immunity, inflammation and disease 11:e852 PubMed37904692

2023

The potential roles of stress-induced phosphoprotein 1 and connexin 43 in rats with reperfusion arrhythmia.

Applications

Unspecified application

Species

Unspecified reactive species

Li An,Hong Gao,Yi Zhong,Yanqiu Liu,Ying Cao,Jing Yi,Xiang Huang,Chunlei Wen,Rui Tong,Zhijun Pan,Xu Yan,Meiyan Liu,Shengzhao Wang,Hao Wu,Tingju Hu

Biophysics and physicobiology 19:e190041 PubMed36349331

2022

Influence of gap junctions upon Ca propagation from stimulated keratinocytes to DRG neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Chiaki Seto,Kenta Toyoda,Kousuke Inada,Kotaro Oka,Etsuro Ito

Bio-protocol 10:e3754 PubMed33659413

2020

Isolation and Expansion of Human Limbal Epithelial Progenitor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Naresh Polisetti,Günther Schlunck,Thomas Reinhard,Friedrich E Kruse,Ursula Schlötzer-Schrehardt

View all publications

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Product promise

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Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (AB235282)

Immunocytochemistry/ Immunofluorescence - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (AB235282)

Immunocytochemistry/ Immunofluorescence - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (AB235282)

Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (AB235282)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise