Anti-Connexin 43 / GJA1 antibody [RM1071] - BSA and Azide free (ab314909)

Knockout Tested Rabbit Multiclonal Connexin 43 / GJA1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, IHC-Fr, IP and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Connexin 43 / GJA1 antibody [RM1071] - BSA and Azide free (AB314909), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF	IHC-Fr	IP	Flow Cyt
Human	Tested	Tested	Tested	Expected	Tested	Not recommended
Mouse	Tested	Tested	Expected	Tested	Tested	Not recommended
Rat	Tested	Tested	Expected	Tested	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Associated Products

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Target data

See full target information GJA1

Function

Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity).

Alternative names

GJAL, GJA1, Gap junction alpha-1 protein, Connexin-43, Gap junction 43 kDa heart protein, Cx43

Recommended products

Knockout Tested Rabbit Multiclonal Connexin 43 / GJA1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, IHC-Fr, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: RM1071
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab314909 is the carrier-free version of Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908.

This product is a recombinant multiclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Connexin 43 also known as GJA1 is a protein that plays an important role in cell communication through gap junctions. It has a molecular weight of approximately 43 kDa. Connexin 43 gets expressed in various tissues including the heart and brain where it facilitates cellular signaling. This protein integrates into cell membranes to form gap junction channels allowing direct transfer of ions and small molecules between adjacent cells. Researchers often use techniques such as Western blot to detect Connexin 43 expression levels in studies of cellular communications.

Biological function summary

This protein facilitates electrical and chemical coupling between cells by forming intercellular channels. These channels arise from the assembly of six Connexin 43 subunits into hemichannels which dock with hemichannels on neighboring cells. This protein has a critical component in the cardiac and neural tissues where rapid communication is essential for proper function. Connexin 43 engages in complex assemblies with other connexins enhancing its role in synchronizing cell activities.

Pathways

Connexin 43 significantly influences signal transduction processes. It acts within the MAPK signaling pathway which impacts cellular responses such as growth and differentiation. Additionally this protein plays a role in calcium signaling a pathway important for muscle contraction and neurotransmitter release. Interacting proteins like Connexin 30 and Connexin 45 further regulate these pathways affecting the amplitude and duration of signaling.

Associated diseases and disorders

Connexin 43 has been linked to cardiac arrhythmias and some forms of cancer. Mutations or altered expression of Connexin 43 can disrupt the electrical conduction in cardiac tissue leading to arrhythmogenic conditions. Research suggests an association with proteins like ZO-1 and Connexin 37 which may affect cell proliferation and migration in cancerous tissues. Understanding these connections highlights Connexin 43's potential as a target for therapeutic interventions in related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

Western blot - Anti-Connexin 43 / GJA1 antibody [RM1071] - BSA and Azide free (ab314909)
This data was developed using Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908, the same antibody clone in a different buffer formulation.
Western blot: Anti-GJA1 antibody [RM1071] (Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 was shown to bind specifically to GJA1. A band was observed at 45 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in GJA1 knockout cell line. To generate this image, wild-type and GJA1 knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-Connexin 43 / GJA1 antibody [RM1071] (Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908) at 1/1000 dilution
Lane 1: Wild-type U-87 MG cell lysate at 20 µg
Lane 2: Western blot - Human GJA1 knockout U-87 MG cell line (Human GJA1 knockout U-87 MG cell line ab306676)
Lane 2: GJA1 knockout U-87 MG cell lysate at 20 µg
Lane 3: Wild-type HEK-293 cell lysate at 20 µg
Lane 4: GJA1 knockout HEK-293 Human GJA1 knockout HEK-293 cell lysate ab261658 cell lysate at 20 µg
Secondary
Lanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution
Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 45 kDa
Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [RM1071] - BSA and Azide free (ab314909)
This data was developed using Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse heart (fresh) tissue labeling Connexin 43 / GJA1 with Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 at 1/100 (5.24 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on mouse heart. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunohistochemistry (Frozen sections) - Anti-Connexin 43 / GJA1 antibody [RM1071] - BSA and Azide free (ab314909)
This data was developed using Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat heart (fresh) tissue labeling Connexin 43 / GJA1 with Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 at 1/100 (5.24 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on rat heart. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Connexin 43 / GJA1 antibody [RM1071] - BSA and Azide free (ab314909)
This data was developed using Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized GJA1 KO HEK293 (GJA1 knockout human embryonic kidney cell line) cells labelling Connexin 43 / GJA1 with Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 at 1/50 (10.48 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in HEK293T cell line, and no staining in GJA1 knockout HEK293T cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [RM1071] - BSA and Azide free (ab314909)
This data was developed using Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) Wild-type HEK293 (human epithelial cell) cell pellet (B)GJA1 KO HEK293 cell pellet tissue labeling Connexin 43 / GJA1 with Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 at 1/100 (5.24 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) Wild-type HEK293 cell pellet, no staining on (B) GJA1 KO HEK293(OG060024) cell pellet. The section was incubated with Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [RM1071] - BSA and Azide free (ab314909)
This data was developed using Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Connexin 43 / GJA1 with Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 at 1/5000 (0.105 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cardiac muscle. The section was incubated with Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [RM1071] - BSA and Azide free (ab314909)
This data was developed using Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Connexin 43 / GJA1 with Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 at 1/5000 (0.105 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cardiac muscle. The section was incubated with Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody [RM1071] - BSA and Azide free (ab314909)
This data was developed using Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Connexin 43 / GJA1 with Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 at 1/500 (1.048 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cardiac muscle. The section was incubated with Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [RM1071] - BSA and Azide free (ab314909)
This data was developed using Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908, the same antibody clone in a different buffer formulation.
Connexin 43 / GJA1 was immunoprecipitated from 0.35 mg Rat brain tissue lysate with Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Rat brain tissue lysate
Lane 2: Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 IP in Rat brain tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 in rat brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [RM1071] (Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908) at 1/30 dilution
All lanes: Rat brain tissue lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 3s
Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [RM1071] - BSA and Azide free (ab314909)
This data was developed using Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908, the same antibody clone in a different buffer formulation.
Connexin 43 / GJA1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 IP in Mouse brain tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 in mouse brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [RM1071] (Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908) at 1/30 dilution
All lanes: Mouse brain tissue lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 3s
Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [RM1071] - BSA and Azide free (ab314909)
This data was developed using Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908, the same antibody clone in a different buffer formulation.
Connexin 43 / GJA1 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2: Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Connexin 43 / GJA1 antibody [RM1071] (Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908) at 1/30 dilution
All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 24s
Western blot - Anti-Connexin 43 / GJA1 antibody [RM1071] - BSA and Azide free (ab314909)
This data was developed using Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile is consistent with the existence of natural variants described in the literature (PMID: 24210816; PMID: 28576298).
Exposure time: Lane 1: 4 seconds; Lanes 2:6 seconds；Lanes 3/5-6: 180 seconds；Lanes 4: 70 seconds；Lanes 7: 37 seconds
All lanes: Western blot - Anti-Connexin 43 / GJA1 antibody [RM1071] (Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse liver tissue lysate at 20 µg
Lane 4: Human heart tissue lysate at 20 µg
Lane 5: Mouse heart tissue lysate at 20 µg
Lane 6: Rat heart tissue lysate at 20 µg
Lane 7: Rat brain tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 43 kDa
Western blot - Anti-Connexin 43 / GJA1 antibody [RM1071] - BSA and Azide free (ab314909)
This data was developed using Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile is consistent with the existence of natural variants described in the literature (PMID: 24210816; PMID: 28576298).
Exposure time: Lane 1: 28 seconds; Lanes 2-4:8 seconds
All lanes: Western blot - Anti-Connexin 43 / GJA1 antibody [RM1071] (Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: Huh7 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg
Lane 4: hTERT NF1 ipNF95.11b (human neural Schwann cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 43 kDa
Western blot - Anti-Connexin 43 / GJA1 antibody [RM1071] - BSA and Azide free (ab314909)
This data was developed using Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of TBS
Western blot: Anti-Connexin 43 / GJA1 antibody (Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5](Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.

In Western blot, Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908 was shown to bind specifically to Connexin 43 / GJA1. Target of interest was observed at 43 kDa in wild-type HEK-293 cell lysates (lane 1) with no signal observed at this size in GJA1 knockout cell line (lane 2, knockout cell line Human GJA1 knockout HEK-293 cell line ab261719/ knockout cell lysate Human GJA1 knockout HEK-293 cell lysate ab261658). To generate this image, samples were first run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in a fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed in TBS-T, incubated with secondary antibodies Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.
All lanes: Western blot - Anti-Connexin 43 / GJA1 antibody [RM1071] (Anti-Connexin 43 / GJA1 antibody [RM1071] ab314908) at 1/1000 dilution
Lane 1: Wild-type HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: Western blot - Human GJA1 knockout HEK-293 cell lysate (Human GJA1 knockout HEK-293 cell lysate ab261658) at 20 µg
Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: Human testis tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG H&L (800CW) and Goat Anti-Mouse IgG H&L (680RD) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 43 kDa, 36 kDa