AB194928

Anti-Connexin 43 / GJA1 (phospho S368) antibody

Name: Anti-Connexin 43 / GJA1 (phospho S368) antibody (ab194928) | Abcam
Brand: Abcam Limited
SKU: AB194928
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal Connexin 43 / GJA1 phospho S368 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human GJA1 phospho S368.

View Alternative Names

GJAL, GJA1, Gap junction alpha-1 protein, Connexin-43, Gap junction 43 kDa heart protein, Cx43

1 Images

Supplier Data

Western blot - Anti-Connexin 43 / GJA1 (phospho S368) antibody (AB194928)

All lanes:

Western blot - Anti-Connexin 43 / GJA1 (phospho S368) antibody (ab194928) at 1/500 dilution

Secondary

Lane 1:

Untreated 293 cell lysate

Lane 2:

PMA treated 293 cell lysate

Predicted band size: 43 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Synthetic Peptide within Human GJA1 phospho S368. The exact immunogen used to generate this antibody is proprietary information.

P17302

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Connexin 43 also known as GJA1 is a protein that plays an important role in cell communication through gap junctions. It has a molecular weight of approximately 43 kDa. Connexin 43 gets expressed in various tissues including the heart and brain where it facilitates cellular signaling. This protein integrates into cell membranes to form gap junction channels allowing direct transfer of ions and small molecules between adjacent cells. Researchers often use techniques such as Western blot to detect Connexin 43 expression levels in studies of cellular communications.

Biological function summary

This protein facilitates electrical and chemical coupling between cells by forming intercellular channels. These channels arise from the assembly of six Connexin 43 subunits into hemichannels which dock with hemichannels on neighboring cells. This protein has a critical component in the cardiac and neural tissues where rapid communication is essential for proper function. Connexin 43 engages in complex assemblies with other connexins enhancing its role in synchronizing cell activities.

Pathways

Connexin 43 significantly influences signal transduction processes. It acts within the MAPK signaling pathway which impacts cellular responses such as growth and differentiation. Additionally this protein plays a role in calcium signaling a pathway important for muscle contraction and neurotransmitter release. Interacting proteins like Connexin 30 and Connexin 45 further regulate these pathways affecting the amplitude and duration of signaling.

Connexin 43 has been linked to cardiac arrhythmias and some forms of cancer. Mutations or altered expression of Connexin 43 can disrupt the electrical conduction in cardiac tissue leading to arrhythmogenic conditions. Research suggests an association with proteins like ZO-1 and Connexin 37 which may affect cell proliferation and migration in cancerous tissues. Understanding these connections highlights Connexin 43's potential as a target for therapeutic interventions in related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity : acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity).

See full target information GJA1 phospho S368

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Arteriosclerosis, thrombosis, and vascular biology 44:e117-e130 PubMed38385289

2024

Inhibition of IL-1 Ameliorates Cardiac Dysfunction and Arrhythmias in a Murine Model of Kawasaki Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Thassio Mesquita,Yen-Nien Lin,Shuang Chen,Youngho Lee,Rodrigo Miguel-Dos-Santos,Asli E Atici,Michael C Fishbein,Magali Noval Rivas,Moshe Arditi,Eugenio Cingolani

Cell biochemistry and function 41:1526-1542 PubMed38014767

2023

An increase in intercellular crosstalk and electrotonic coupling between cardiomyocytes and nonmyocytes reshapes the electrical conduction in the metabolic heart characterized by short QT intervals in ECGs.

Applications

Unspecified application

Species

Unspecified reactive species

Deniz Billur,Yusuf Olgar,Aysegul Durak,Ayse Hande Yozgat,Simge Unay,Erkan Tuncay,Belma Turan

Translational andrology and urology 11:1486-1494 PubMed36507487

2022

Glycated serum albumin decreases connexin 43 phosphorylation in the corpus cavernosum.

Applications

Unspecified application

Species

Unspecified reactive species

Yanping Huang,Wei Liu,Yidong Liu,Ming Zhang,Xiangguo Lv,Kai Hu,Shiran Xu,Mujun Lu

Naunyn-Schmiedeberg's archives of pharmacology 395:1097-1107 PubMed35776167

2022

Therapeutic effects of a lipid transfer protein isolated from Morinda citrifolia L. (noni) seeds on irinotecan-induced intestinal mucositis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Luana David do Carmo,Gisele de Fátima Pinheiro Rangel,Liviane Maria Alves Rabelo,Tamiris de Fátima Goebel de Souza,Roberto César Pereira Lima Júnior,Deysi Viviana Tenazoa Wong,Renata Ferreira de Carvalho Leitão,Alfredo Augusto Vasconcelos da Silva,Pedro Jorge Caldas Magalhães,Andréa Santos Costa,Dyély de Carvalho Oliveira Campos,Nylane Maria Nunes de Alencar,Hermógenes David de Oliveira

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 70:447-462 PubMed35608408

2022

Intracellular Redistribution of Left Ventricular Connexin 43 Contributes to the Remodeling of Electrical Properties of the Heart in Insulin-resistant Elderly Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Deniz Billur,Yusuf Olgar,Belma Turan

BioMed research international 2020:3451215 PubMed32258113

2020

Isoflurane Postconditioning Upregulates Phosphorylated Connexin 43 in the Middle Cerebral Artery Occlusion Model and Is Probably Associated with the TGF-1/Smad2/3 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangwen Yin,Xuejiao Liu,Ruixue Wang,Mingyue Ge,Liping Xie,Jingwen Zhai,Zhigang Dai,Yan Li,Sheng Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Anti-Connexin 43 / GJA1 (phospho S368) antibody

Western blot - Anti-Connexin 43 / GJA1 (phospho S368) antibody (AB194928)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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