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Rabbit Polyclonal Connexin 43 / GJA1 phospho S368 antibody. Suitable for WB and reacts with Rat, Mouse, Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Rat Gja1 phospho S368.


Images

Western blot - Anti-Connexin 43 / GJA1 (phospho S368) antibody (AB30559), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.238% HEPES, 0.01% BSA

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Synthetic Peptide within Rat Gja1 phospho S368. The exact immunogen used to generate this antibody is proprietary information. Database link P08050

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Expected
Mouse
Expected
Rat
Tested
Primates
Predicted

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

The immunolabeling of Connexin43 is completely eliminated by treatment with lambda phosphatase.

Expected
Expected

Species
Mouse
Dilution info
1/1000
Notes

The immunolabeling of Connexin43 is completely eliminated by treatment with lambda phosphatase.

Species
Human
Dilution info
1/1000
Notes

The immunolabeling of Connexin43 is completely eliminated by treatment with lambda phosphatase.

Predicted
Predicted

Species
Primates
Dilution info
-
Notes

-

Associated Products

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Target data

Function

Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization (PubMed:15181016). Plays an essential role in gap junction communication in the ventricles (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal Connexin 43 / GJA1 phospho S368 antibody. Suitable for WB and reacts with Rat, Mouse, Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Rat Gja1 phospho S368.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Synthetic Peptide within Rat Gja1 phospho S368. The exact immunogen used to generate this antibody is proprietary information. Database link P08050
Purification technique
Affinity purification Immunogen
Specificity

Specific for ~43k Connexin43 protein phosphorylated at Ser368.

Concentration
Loading...
Purification notes

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Connexin 43 also known as GJA1 is a protein that plays an important role in cell communication through gap junctions. It has a molecular weight of approximately 43 kDa. Connexin 43 gets expressed in various tissues including the heart and brain where it facilitates cellular signaling. This protein integrates into cell membranes to form gap junction channels allowing direct transfer of ions and small molecules between adjacent cells. Researchers often use techniques such as Western blot to detect Connexin 43 expression levels in studies of cellular communications.

Biological function summary

This protein facilitates electrical and chemical coupling between cells by forming intercellular channels. These channels arise from the assembly of six Connexin 43 subunits into hemichannels which dock with hemichannels on neighboring cells. This protein has a critical component in the cardiac and neural tissues where rapid communication is essential for proper function. Connexin 43 engages in complex assemblies with other connexins enhancing its role in synchronizing cell activities.

Pathways

Connexin 43 significantly influences signal transduction processes. It acts within the MAPK signaling pathway which impacts cellular responses such as growth and differentiation. Additionally this protein plays a role in calcium signaling a pathway important for muscle contraction and neurotransmitter release. Interacting proteins like Connexin 30 and Connexin 45 further regulate these pathways affecting the amplitude and duration of signaling.

Associated diseases and disorders

Connexin 43 has been linked to cardiac arrhythmias and some forms of cancer. Mutations or altered expression of Connexin 43 can disrupt the electrical conduction in cardiac tissue leading to arrhythmogenic conditions. Research suggests an association with proteins like ZO-1 and Connexin 37 which may affect cell proliferation and migration in cancerous tissues. Understanding these connections highlights Connexin 43's potential as a target for therapeutic interventions in related diseases.

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1 product image

  • Western blot - Anti-Connexin 43 / GJA1 (phospho S368) antibody (ab30559), expandable thumbnail

    Western blot - Anti-Connexin 43 / GJA1 (phospho S368) antibody (ab30559)

    The phosphospecificity of this labeling is shown in the second lane which was incubated in lambda phosphatase (1200 units for 30 min) before being exposed to GJA1.

    All lanes: Western blot - Anti-Connexin 43 / GJA1 (phospho S368) antibody (ab30559) at 1/1000 dilution

    Lane 1: Rat hippocampal lysate at 10 µg

    Lane 2: Rat hippocampal lysate (lambda phosphatase treated) at 10 µg

    Predicted band size: 43 kDa

    Observed band size: 43 kDa

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