AB30559

Anti-Connexin 43 / GJA1 (phospho S368) antibody

Name: Anti-Connexin 43 / GJA1 (phospho S368) antibody (ab30559) | Abcam
Brand: Abcam Limited
SKU: AB30559
Price: 535 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(15 Publications)

Rabbit Polyclonal Connexin 43 / GJA1 phospho S368 antibody. Suitable for WB and reacts with Rat, Mouse, Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Rat Gja1 phospho S368.

View Alternative Names

Cxn-43, Gap junction alpha-1 protein, Connexin-43, Gap junction 43 kDa heart protein, Cx43

1 Images

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Western blot - Anti-Connexin 43 / GJA1 (phospho S368) antibody (AB30559)

The phosphospecificity of this labeling is shown in the second lane which was incubated in lambda phosphatase (1200 units for 30 min) before being exposed to GJA1.

All lanes:

Western blot - Anti-Connexin 43 / GJA1 (phospho S368) antibody (ab30559) at 1/1000 dilution

Lane 1:

Rat hippocampal lysate at 10 µg

Lane 2:

Rat hippocampal lysate (lambda phosphatase treated) at 10 µg

Predicted band size: 43 kDa

Observed band size: 43 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Rat Gja1 phospho S368. The exact immunogen used to generate this antibody is proprietary information.

P08050

Specificity

Specific for ~43k Connexin43 protein phosphorylated at Ser368.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "The immunolabeling of Connexin43 is completely eliminated by treatment with lambda phosphatase." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "The immunolabeling of Connexin43 is completely eliminated by treatment with lambda phosphatase." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "The immunolabeling of Connexin43 is completely eliminated by treatment with lambda phosphatase." }, "Primates": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.

Storage buffer

pH: 7.5 Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.238% HEPES, 0.01% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Connexin 43 also known as GJA1 is a protein that plays an important role in cell communication through gap junctions. It has a molecular weight of approximately 43 kDa. Connexin 43 gets expressed in various tissues including the heart and brain where it facilitates cellular signaling. This protein integrates into cell membranes to form gap junction channels allowing direct transfer of ions and small molecules between adjacent cells. Researchers often use techniques such as Western blot to detect Connexin 43 expression levels in studies of cellular communications.

Biological function summary

This protein facilitates electrical and chemical coupling between cells by forming intercellular channels. These channels arise from the assembly of six Connexin 43 subunits into hemichannels which dock with hemichannels on neighboring cells. This protein has a critical component in the cardiac and neural tissues where rapid communication is essential for proper function. Connexin 43 engages in complex assemblies with other connexins enhancing its role in synchronizing cell activities.

Pathways

Connexin 43 significantly influences signal transduction processes. It acts within the MAPK signaling pathway which impacts cellular responses such as growth and differentiation. Additionally this protein plays a role in calcium signaling a pathway important for muscle contraction and neurotransmitter release. Interacting proteins like Connexin 30 and Connexin 45 further regulate these pathways affecting the amplitude and duration of signaling.

Connexin 43 has been linked to cardiac arrhythmias and some forms of cancer. Mutations or altered expression of Connexin 43 can disrupt the electrical conduction in cardiac tissue leading to arrhythmogenic conditions. Research suggests an association with proteins like ZO-1 and Connexin 37 which may affect cell proliferation and migration in cancerous tissues. Understanding these connections highlights Connexin 43's potential as a target for therapeutic interventions in related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Structural component of the gap junction, a specialized intercellular structure consisting of a cluster of closely packed pairs of transmembrane channels, the connexons, that allow passage of small molecules and electrical signals between neighboring cells. Forms homotypic and heterotypic channels gated by transjunctional voltage (PubMed : 12011467). May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph (By similarity). Negative regulator of bladder functional capacity : acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in the conductive system of ventricular myocardium and heart morphogenesis (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization (PubMed : 15181016). Involved in intercellular innate immune signaling. Mediates translocation of 2',3'-cGAMP and 2',5'-oligoadenylates (2-5A) second messengers from virus-infected cells to macrophages and uninfected neighboring cells to propagate and amplify the antiviral immune response (By similarity).

See full target information Gja1 phospho S368

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 49:92-100 PubMed39400417

2024

Rotigaptide inhibits spontaneous contractions of gastric smooth muscle in diabetic rats via the PKCα-Cx43 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Changri,Haibei Sun,Yitegele Bao,Mohan Zhang

Investigative ophthalmology & visual science 64:30 PubMed37982762

2023

O-GlcNAcylation Inhibition Upregulates Connexin43 Expression in the Endothelium to Protect the Tight Junction Barrier in Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Guodong Liu,Le Feng,Xiaoqiang Liu,Peng Gao,Fang Wang

Translational andrology and urology 11:1486-1494 PubMed36507487

2022

Glycated serum albumin decreases connexin 43 phosphorylation in the corpus cavernosum.

Applications

Unspecified application

Species

Unspecified reactive species

Yanping Huang,Wei Liu,Yidong Liu,Ming Zhang,Xiangguo Lv,Kai Hu,Shiran Xu,Mujun Lu

Frontiers in molecular neuroscience 15:859558 PubMed35966011

2022

NF-κB and AP-1 are required for the lipopolysaccharide-induced expression of MCP-1, CXCL1, and Cx43 in cultured rat dorsal spinal cord astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Lu,Bo Li,Axiang Xu,Xuan Liang,Tao Xu,Huan Jin,Ye Xie,Rong Wang,Xiaohong Liu,Xiaohong Gao,Yong Han,Junwei Zeng

Neural regeneration research 17:594-600 PubMed34380899

2021

The mechanisms through which auricular vagus nerve stimulation protects against cerebral ischemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Jun Zhao,Zheng-Hui Wang,Ying-Jie Zhang,Wen-Jing Wang,Ai-Fang Cheng,Pei-Jing Rong,Chun-Lei Shan

Frontiers in cell and developmental biology 9:637233 PubMed34169070

2021

Propofol Mediated Protection of the Brain From Ischemia/Reperfusion Injury Through the Regulation of Microglial Connexin 43.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Zhang,Yanyan Wang,Qin Xia,Zhiyi Tu,Jiajun Sun,Qi Jing,Pei Chen,Xuan Zhao

The Journal of pharmacology and experimental therapeutics 376:444-453 PubMed33384302

2021

Novel Tocolytic Strategy: Modulating Cx43 Activity by -Nitrosation.

Applications

Unspecified application

Species

Unspecified reactive species

Scott D Barnett,Hazik Asif,Mitchell Anderson,Iain L O Buxton

American journal of physiology. Cell physiology 317:C825-C842 PubMed31365296

2019

The lipidated connexin mimetic peptide SRPTEKT- is a potent inhibitor of Cx43 channels with specificity for the pS368 phospho-isoform.

Applications

Unspecified application

Species

Unspecified reactive species

Maura L Cotter,Scott Boitano,Paul D Lampe,Joell L Solan,Josef Vagner,Jose F Ek-Vitorin,Janis M Burt

Journal of neuroinflammation 15:97 PubMed29587860

2018

Roles of astrocytic connexin-43, hemichannels, and gap junctions in oxygen-glucose deprivation/reperfusion injury induced neuroinflammation and the possible regulatory mechanisms of salvianolic acid B and carbenoxolone.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Yin,Liangshu Feng,Di Ma,Ping Yin,Xinyu Wang,Shuai Hou,Yulei Hao,Jingdian Zhang,Meiying Xin,Jiachun Feng

Frontiers in cellular neuroscience 11:207 PubMed28769766

2017

Breakthrough Cancer Pain Is Associated with Spinal Gap Junction Activation via Regulation of Connexin 43 in a Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Siqing Jiang,Hui Yang,Qian Liao,Shousong Cao,Xuebin Yan,Dong Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Anti-Connexin 43 / GJA1 (phospho S368) antibody

Western blot - Anti-Connexin 43 / GJA1 (phospho S368) antibody (AB30559)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise