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Rabbit Recombinant Monoclonal Connexin 43 / GJA1 phospho Y265 antibody. Suitable for WB, Dot, ICC/IF, IP and reacts with Transfected cell lysate - Human, Human, Transfected cell lysate - Mouse, Synthetic peptide, Transfected cell line - Human samples.

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Images

Western blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (AB312836), expandable thumbnail
  • Western blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (AB312836), expandable thumbnail
  • Western blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (AB312836), expandable thumbnail
  • Dot Blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (AB312836), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (AB312836), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBDotICC/IFIPIHC-PFlow Cyt (Intra)
Human
Tested
Expected
Tested
Tested
Not recommended
Not recommended
Mouse
Predicted
Predicted
Predicted
Predicted
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Synthetic peptide
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell line - Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Transfected cell lysate - Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

(0.484ug/ml)

Species
Human
Dilution info
1/1000
Notes

(0.484ug/ml)

Species
Transfected cell lysate - Mouse
Dilution info
1/1000
Notes

(0.484ug/ml)

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide, Transfected cell line - Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human, Transfected cell lysate - Mouse, Transfected cell line - Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/8000
Notes

-

Species
Transfected cell line - Human
Dilution info
1/8000
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human, Transfected cell lysate - Mouse, Synthetic peptide, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Species
Transfected cell lysate - Human
Dilution info
1/30
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Mouse, Synthetic peptide, Transfected cell line - Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human, Transfected cell lysate - Mouse, Synthetic peptide, Transfected cell line - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Transfected cell lysate - Human, Transfected cell lysate - Mouse, Synthetic peptide, Transfected cell line - Human, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity).

Alternative names

GJAL, GJA1, Gap junction alpha-1 protein, Connexin-43, Gap junction 43 kDa heart protein, Cx43

Recommended products

Rabbit Recombinant Monoclonal Connexin 43 / GJA1 phospho Y265 antibody. Suitable for WB, Dot, ICC/IF, IP and reacts with Transfected cell lysate - Human, Human, Transfected cell lysate - Mouse, Synthetic peptide, Transfected cell line - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26416-134
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Connexin 43 also known as GJA1 is a protein that plays an important role in cell communication through gap junctions. It has a molecular weight of approximately 43 kDa. Connexin 43 gets expressed in various tissues including the heart and brain where it facilitates cellular signaling. This protein integrates into cell membranes to form gap junction channels allowing direct transfer of ions and small molecules between adjacent cells. Researchers often use techniques such as Western blot to detect Connexin 43 expression levels in studies of cellular communications.

Biological function summary

This protein facilitates electrical and chemical coupling between cells by forming intercellular channels. These channels arise from the assembly of six Connexin 43 subunits into hemichannels which dock with hemichannels on neighboring cells. This protein has a critical component in the cardiac and neural tissues where rapid communication is essential for proper function. Connexin 43 engages in complex assemblies with other connexins enhancing its role in synchronizing cell activities.

Pathways

Connexin 43 significantly influences signal transduction processes. It acts within the MAPK signaling pathway which impacts cellular responses such as growth and differentiation. Additionally this protein plays a role in calcium signaling a pathway important for muscle contraction and neurotransmitter release. Interacting proteins like Connexin 30 and Connexin 45 further regulate these pathways affecting the amplitude and duration of signaling.

Associated diseases and disorders

Connexin 43 has been linked to cardiac arrhythmias and some forms of cancer. Mutations or altered expression of Connexin 43 can disrupt the electrical conduction in cardiac tissue leading to arrhythmogenic conditions. Research suggests an association with proteins like ZO-1 and Connexin 37 which may affect cell proliferation and migration in cancerous tissues. Understanding these connections highlights Connexin 43's potential as a target for therapeutic interventions in related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836), expandable thumbnail

    Western blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836)

    Connexin 43 / GJA1 (phospho Y265) Western blot staining using rabbit Anti-Connexin 43 / GJA1 (phospho Y265) antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18649180; PMID: 30448479).

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    Alkaline phosphatase. -: untreated membrane; +: phosphatase treated membrane.

    In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    Anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Anti-Connexin 43 / GJA1 antibody [EPR21153] (Anti-Connexin 43 / GJA1 antibody [EPR21153] - Intercellular Junction Marker ab217676) total protein control staining at 1/1000 dilution.

    All lanes: Western blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) cells transfected with an empty vector containi a myc-His-tag® whole cell lysate at 20 µg

    Lane 2: HeLa cells transfected with a human GJA1 expression vector containi a myc-His-tag® whole cell lysate at 20 µg

    Lane 3: HeLa cells transfected with a human GJA1(Mutant Y265F) expression vector containi a myc-His-tag® whole cell lysate at 20 µg

    Lane 4: HeLa cells transfected with a RSV-SR-A V-SRC expression vector containi a myc-His-tag® whole cell lysate at 20 µg

    Lane 5: HeLa cells co-transfected with a human GJA1 expression vector containi a myc-His-tag® and a RSV-SR-A V-SRC expression vector containi a myc-His-tag® whole cell lysate at 20 µg

    Lane 6: HeLa cells co-transfected with a human GJA1(Mutant Y265F) expression vector containi a myc-His-tag® and a RSV-SR-A V-SRC expression vector containi a myc-His-tag® whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 43 kDa

    Exposure time: 92s

  • Western blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836), expandable thumbnail

    Western blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836)

    Connexin 43 / GJA1 (phospho Y265) Western blot staining using rabbit Anti-Connexin 43 / GJA1 (phospho Y265) antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:15371442，PMID: 30054380).

    All lanes: Western blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836) at 1/1000 dilution

    Lane 1: Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa treated with 1mM pervanadate for 60 minutes whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Observed band size: 43 kDa

    Exposure time: 60s

  • Western blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836), expandable thumbnail

    Western blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    Alkaline phosphatase. -: untreated membrane; +: phosphatase treated membrane.

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836) at 1/1000 dilution

    Lane 1: Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa treated with 30mM pervanadate for 30 minutes whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 43 kDa

    Exposure time: 180s

  • Dot Blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836), expandable thumbnail

    Dot Blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836)

    Dot blot analysis of Connexin 43 / GJA1 (phospho Y265) using ab312836 at 1:1000 (0.484 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
    Lane 1: Connexin 43 / GJA1 (phospho Y265) peptide a
    Lane 2: Connexin 43 / GJA1 (phospho Y265) peptide b
    Lane 3: Connexin 43 / GJA1 non-phospho peptide
    Exposure time: 180 seconds
    Blocking and diluting buffer and concentration: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Connexin 43 / GJA1 (phospho Y265) with ab312836 at 1/8000 (0.061 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic and membranous staining in HeLa cells transfected with a human Connexin-43 wildtype and V-SRC wildtype expression vectors containing a myc tag and showing no staining in HeLa cells transfected with a human Connexin-43 Y265F and V-SRC wildtype expression vectors containing a myc tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 (0.38 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunoprecipitation - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836), expandable thumbnail

    Immunoprecipitation - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836)

    Connexin 43 / GJA1 (phospho Y265) was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) cells co-transfected with a human GJA1 expression vector containing a myc-His-tag® and a RSV-SR-A V-SRC expression vector containing a myc-His-tag® whole cell lysate with ab312836 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab312836 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) cells co-transfected with a human GJA1 expression vector containing a myc-His-tag® and a RSV-SR-A V-SRC expression vector containing a myc-His-tag® whole cell lysate
    Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) cells co-transfected with a human GJA1 expression vector containing a myc-His-tag® and a RSV-SR-A V-SRC expression vector containing a myc-His-tag® whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab312836 in HeLa cells co-transfected with a human GJA1 expression vector containing a myc-His-tag® and a RSV-SR-A V-SRC expression vector containing a myc-His-tag® whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 8 seconds

    All lanes: Immunoprecipitation - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836) at 1/30 dilution

    All lanes: HeLa cells co-transfected with a human GJA1 expression vector containing a myc-His-tag® and a RSV-SR-A V-SRC expression vector containing a myc-His-tag® whole cell lysate at 2 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 8s

  • Western blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836), expandable thumbnail

    Western blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18649180; PMID: 30448479).
    Alkaline phosphatase. -: untreated membrane; +: phosphatase treated membrane.
    In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
    Anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Anti-Connexin 43 / GJA1 antibody [EPR21153] (Anti-Connexin 43 / GJA1 antibody [EPR22955-101] - Intercellular Junction Marker ab235585) total protein control staining at 1/1000 dilution.
    Exposure time: 70 seconds

    All lanes: Western blot - Anti-Connexin 43 / GJA1 (phospho Y265) antibody [EPR26416-134] (ab312836) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) cells transfected with an empty vector containi a myc-His-tag® whole cell lysate at 20 µg

    Lane 2: HeLa cells transfected with a RSV-SR-A V-SRC expression vector containi a myc-His-tag® whole cell lysate at 20 µg

    Lane 3: HeLa cells transfected with a mouse GJA1 expression vector containi a myc-His-tag® whole cell lysate at 20 µg

    Lane 4: HeLa cells co-transfected with a mouse GJA1 expression vector containi a myc-His-tag® and a RSV-SR-A V-SRC expression vector containi a myc-His-tag® whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 48 kDa, 55 kDa

    Exposure time: 70s

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Product protocols

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