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AB78408

Anti-Connexin 45/GJA7/Cx45 antibody [5B9.2]

5

(2 Reviews)

|

(10 Publications )

Mouse Monoclonal Connexin 45/GJA7/Cx45 antibody. Suitable for ICC, Flow Cyt and reacts with Rat, Human samples. Cited in 10 publications.
2 Images
Flow Cytometry - Anti-Connexin 45/GJA7/Cx45 antibody [5B9.2] (AB78408)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Connexin 45/GJA7/Cx45 antibody [5B9.2] (AB78408)

Overlay histogram showing HeLa cells stained with ab78408 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78408, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

This image was generated using the ascites version of the product.

Immunocytochemistry - Anti-Connexin 45/GJA7/Cx45 antibody [5B9.2] (AB78408)
  • ICC

Unknown

Immunocytochemistry - Anti-Connexin 45/GJA7/Cx45 antibody [5B9.2] (AB78408)

ab78408 staining Connexin 45/GJA7/Cx45 transfected rat C6 cells by Immunocytochemistry.

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5B9.2

Isotype

IgG1

Carrier free

No

Reacts with

Rat, Human

Applications

Flow Cyt, ICC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

Near C terminal, cytoplasmic.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Chinese hamster": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

This product was changed from ascites to tissue culture supernatant on 17th May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Purification notes
Purified from TCS.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1.45% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Connexin 45 also known as Cx45 or GJA7 is a gap junction protein that enables cellular communication by forming channels between adjacent cells. The channels regulate ion and small molecule passage which is critical for maintaining cellular homeostasis. Cx45 has a molecular weight of approximately 45 kDa. This protein expresses in various tissues including the heart brain and smooth muscle indicating its diverse physiological roles.
Biological function summary

Cx45 plays an integral role in intercellular communication by participating in the formation of gap junction channels. It functions as part of the connexin family forming homomeric and heteromeric hemichannels that dock with hemichannels on adjacent cells. This connection is essential for electronic coupling and synchronous activity especially in the cardiac and neural tissues where timely communication is necessary for function.

Pathways

Cx45 contributes significantly to the cardiac conduction system and neural transmission pathways. In cardiac tissues it coordinates with connexin 43 to control electrical signal propagation and regulate heartbeat. Cx45 participates in neural pathways by forming functional networks that support synaptic transmission and neuronal synchronization thereby interacting with proteins like connexin 36.

Mutations or dysregulation of Cx45 associate with cardiac arrhythmias and neurological disorders like epilepsy. In the heart Cx45 along with connexin 43 is essential for maintaining normal cardiac rhythm and disturbances can lead to arrhythmogenic conditions. In the nervous system Cx45 dysfunction can affect neural circuitry contributing to epileptic seizures. It is vital to investigate the role of Cx45 in these conditions for developing therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Structural component of the gap junction, a specialized intercellular structure consisting of a cluster of closely packed pairs of transmembrane channels, the connexons, that allow passage of small molecules and electrical signals between neighboring cells. Forms homotypic and heterotypic channels gated by transjunctional voltage (By similarity). Involved in intercellular innate immune signaling. Mediates translocation of 2',3'-cGAMP and 2',5'-oligoadenylates (2-5A) second messengers from virus-infected cells to macrophages and uninfected neighboring cells to propagate and amplify the antiviral immune response (PubMed : 24077100, PubMed : 31992625, PubMed : 40010341).
See full target information GJC1

Alternative Names

GJA7, GJC1, Gap junction gamma-1 protein, Connexin-45, Gap junction alpha-7 protein, Cx45
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

The journal of gene medicine 25:e3499 PubMed36908084

2023

Disrupting methyl-CpG-binding protein 2 expression induces the transformation of induced pluripotent stem cell cardiomyocytes into pacemaker-like cells by insulin gene enhancer binding protein 1.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Jianjun Gu,Yanxi Shi,Bichun Li,Xiang Gu

Cells 11: PubMed36078111

2022

Inhibition of Gap Junctional Intercellular Communication Upregulates Pluripotency Gene Expression in Endogenous Pluripotent Muse Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Khaled Hatabi,Yukari Hirohara,Yoshihiro Kushida,Yasumasa Kuroda,Shohei Wakao,James Trosko,Mari Dezawa

Frontiers in cardiovascular medicine 8:690092 PubMed34621795

2021

Study on Optimal Parameter and Target for Pulsed-Field Ablation of Atrial Fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Xuying Ye,Shangzhong Liu,Huijuan Yin,Qiang He,Zhixiao Xue,Chengzhi Lu,Siying Su

Nature communications 11:4283 PubMed32883967

2020

In vitro generation of functional murine heart organoids via FGF4 and extracellular matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyoung Lee,Akito Sutani,Rin Kaneko,Jun Takeuchi,Tetsuo Sasano,Takashi Kohda,Kensuke Ihara,Kentaro Takahashi,Masahiro Yamazoe,Tomohiro Morio,Tetsushi Furukawa,Fumitoshi Ishino

Heart and vessels 35:1025-1035 PubMed32270355

2020

Vascular smooth muscle cell phenotypic transition regulates gap junctions of cardiomyocyte.

Applications

Unspecified application

Species

Unspecified reactive species

En Zhou,Tiantian Zhang,Changlong Bi,Changqian Wang,Zongqi Zhang

American journal of translational research 10:2511-2528 PubMed30210689

2018

Transcription factor Tbx18 induces the differentiation of c-kit canine mesenchymal stem cells (cMSCs) into SAN-like pacemaker cells in a co-culture model in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Xiao,Yong-Jun Yang,Yi-Zhang Lin,Song Peng,Shu Lin,Zhi-Yuan Song

Experimental and therapeutic medicine 16:2960-2968 PubMed30233668

2018

Propofol-induced vasodilation of mesenteric arterioles via BK channel and gap junction.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Juan Wan,Yang Wang,Jun-Qiang Si,Li Li

Oncogene 36:4538-4550 PubMed28368405

2017

Connexin-43 channels are a pathway for discharging lactate from glycolytic pancreatic ductal adenocarcinoma cells.

Applications

WB

Species

Unspecified reactive species

T H Dovmark,M Saccomano,A Hulikova,F Alves,P Swietach

Molecular medicine reports 15:1823-1831 PubMed28259990

2017

Role of gap junctions in the contractile response to agonists in the mesenteric resistance artery of rats with acute hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Liu,Xin-Zhi Li,Min Peng,Wei Ji,Lei Zhao,Li Li,Liang Zhang,Jun-Qiang Si,Ke-Tao Ma

Canadian journal of physiology and pharmacology 91:157-64 PubMed23458200

2013

Connexins and steroidogenesis in mouse Leydig cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Poonampreet Sekhon,Kevin J Barr,Lucrecia Márquez-Rosado,Paul D Lampe,Gerald M Kidder
View all publications
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Product promise

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