AB316743

Anti-Connexin 45/GJA7/Cx45 antibody [EPR26705-152] - BSA and Azide free

Recombinant
RabMAb
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Rabbit Recombinant Monoclonal Connexin 45/GJA7/Cx45 antibody. Carrier free. Suitable for WB, IP and reacts with Human samples.

View Alternative Names

GJA7, GJC1, Gap junction gamma-1 protein, Connexin-45, Gap junction alpha-7 protein, Cx45

2 Images

Supplier Data

Immunoprecipitation - Anti-Connexin 45/GJA7/Cx45 antibody [EPR26705-152] - BSA and Azide free (AB316743)

This data was developed using ab316742, the same antibody clone in a different buffer formulation.

Connexin 45/GJA7/Cx45 was immunoprecipitated from 0.35 mg IMR-32 (human neuroblastoma neuroblast) whole cell lysate with ab316742 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316742 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : IMR-32 (human neuroblastoma neuroblast) whole cell lysate
Lane 2 : ab316742 IP in IMR-32 (human neuroblastoma neuroblast) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab316742 in IMR-32 whole cell lysate

All lanes:

Immunoprecipitation - Anti-Connexin 45/GJA7/Cx45 antibody [EPR26705-152] (<a href='/en-us/products/primary-antibodies/connexin-45-gja7-cx45-antibody-epr26705-152-ab316742'>ab316742</a>) at 1/30 dilution

All lanes:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate with 5% NFDM/TBST

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 8s

Supplier Data

Western blot - Anti-Connexin 45/GJA7/Cx45 antibody [EPR26705-152] - BSA and Azide free (AB316743)

This data was developed using ab316742, the same antibody clone in a different buffer formulation.

Negative control : MCF7, HT-29.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 180 seconds； Lanes 2-5 : 37 seconds.

All lanes:

Western blot - Anti-Connexin 45/GJA7/Cx45 antibody [EPR26705-152] (<a href='/en-us/products/primary-antibodies/connexin-45-gja7-cx45-antibody-epr26705-152-ab316742'>ab316742</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 2:

NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 3:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 4:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 5:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 45 kDa,36 kDa

false

Unconjugated

Anti-Connexin 45/GJA7/Cx45 antibody [EPR26705-152]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26705-152

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab316743 is the carrirer-free version of ab316742.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Connexin 45 also known as Cx45 or GJA7 is a gap junction protein that enables cellular communication by forming channels between adjacent cells. The channels regulate ion and small molecule passage which is critical for maintaining cellular homeostasis. Cx45 has a molecular weight of approximately 45 kDa. This protein expresses in various tissues including the heart brain and smooth muscle indicating its diverse physiological roles.

Biological function summary

Cx45 plays an integral role in intercellular communication by participating in the formation of gap junction channels. It functions as part of the connexin family forming homomeric and heteromeric hemichannels that dock with hemichannels on adjacent cells. This connection is essential for electronic coupling and synchronous activity especially in the cardiac and neural tissues where timely communication is necessary for function.

Pathways

Cx45 contributes significantly to the cardiac conduction system and neural transmission pathways. In cardiac tissues it coordinates with connexin 43 to control electrical signal propagation and regulate heartbeat. Cx45 participates in neural pathways by forming functional networks that support synaptic transmission and neuronal synchronization thereby interacting with proteins like connexin 36.

Mutations or dysregulation of Cx45 associate with cardiac arrhythmias and neurological disorders like epilepsy. In the heart Cx45 along with connexin 43 is essential for maintaining normal cardiac rhythm and disturbances can lead to arrhythmogenic conditions. In the nervous system Cx45 dysfunction can affect neural circuitry contributing to epileptic seizures. It is vital to investigate the role of Cx45 in these conditions for developing therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

See full target information GJC1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Connexin 45/GJA7/Cx45 antibody [EPR26705-152] - BSA and Azide free

Immunoprecipitation - Anti-Connexin 45/GJA7/Cx45 antibody [EPR26705-152] - BSA and Azide free (AB316743)

Western blot - Anti-Connexin 45/GJA7/Cx45 antibody [EPR26705-152] - BSA and Azide free (AB316743)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise