AB223217

Anti-Contactin 1 antibody

Name: Anti-Contactin 1 antibody (ab223217) | Abcam
Brand: Abcam Limited
SKU: AB223217
Price: 525 USD
Availability: InStock

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(2 Publications)

Goat Polyclonal Contactin 1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CNTN1 aa 850 to C-terminus.

View Alternative Names

Contactin-1, Glycoprotein gp135, Neural cell surface protein F3, CNTN1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Contactin 1 antibody (AB223217)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Contactin 1 antibody (AB223217)

Paraffin embedded mouse cerebral cortex tissue stained for Contactin 1 with ab223217 at 5 μg/ml. DAB-staining.

Supplier Data

Western blot - Anti-Contactin 1 antibody (AB223217)

Primary incubation : 1 hour

All lanes:

Western blot - Anti-Contactin 1 antibody (ab223217) at 1 µg/mL

All lanes:

human cerebellum lysate in RIPA buffer at 35 µg

Predicted band size: 113 kDa

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Supplier Data

Western blot - Anti-Contactin 1 antibody (AB223217)

Primary incubation : 1 hour

All lanes:

Western blot - Anti-Contactin 1 antibody (ab223217) at 1 µg/mL

Lane 1:

Mouse brain lysate in RIPA buffer at 35 µg

Lane 2:

Rat brain lysate in RIPA buffer at 35 µg

Predicted band size: 113 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human CNTN1 aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q12860

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-10 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-10 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Contactin 1 also known as contactin-1 or contactin is a protein involved in cell adhesion processes. It is sometimes referred to as contacton. This protein has a molecular weight of approximately 120 kDa and is a neural membrane glycoprotein. Contactin 1 primarily expresses in the developing nervous system and persists in the adult central nervous system. It plays an important role in forming connections between neurons and ensuring proper communication between them.

Biological function summary

The role of this protein involves maintaining neuronal network organization and integrity. Contactin 1 plays a critical part in myelination a process essential for the swift conduction of nerve impulses. It interacts with several partners forming complexes essential for neuronal stability including neurofascin and contactin-associated protein-like 2. These interactions facilitate proper signal transmission across synapses in the nervous system.

Pathways

Contactin 1 is an important element in the pathways that regulate neuronal development and myelination. It is involved in the formation of the nodes of Ranvier important for nerve impulse transmission. Contactin 1 interacts with proteins such as tenascin-R and NrCAM in these processes contributing significantly to the formation and maintenance of functional neural networks.

Defects in the regulation or expression of contactin 1 are associated with neurological conditions like multiple sclerosis and Guillain-Barré syndrome. These disorders highlight problems in nerve signal transmission and immune system misregulation. Through these diseases contactin 1's interaction with proteins such as CASPR (contactin-associated protein-like 2) becomes important as disruptions in these interactions can lead to neuronal dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth (By similarity).

See full target information CNTN1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Translational cancer research 14:3690-3701 PubMed40687216

2025

CNTN1 promotes cell proliferation and metastasis in ovarian cancer through PSEN1.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiying Qi,Yuqing Ji,Yanying Xu,Ruimeng Guo,Xuewang Guo,Yu Dou,Yingying Yue,Fang Wang

Frontiers in oncology 10:583566 PubMed33194722

2020

LRH1 Promotes Tumor Cell Proliferation and Migration and Is Correlated With Poor Prognosis in Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenzhou Sun,Qingtao Shi,Jiaxin Li,Jinmeng Li,Libo Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Contactin 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Contactin 1 antibody (AB223217)

Western blot - Anti-Contactin 1 antibody (AB223217)

Western blot - Anti-Contactin 1 antibody (AB223217)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise