AB66265

Anti-Contactin 1 antibody

(2 Reviews)

(5 Publications)

Rabbit Polyclonal Contactin 1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 5 publications.

View Alternative Names

Contactin-1, Glycoprotein gp135, Neural cell surface protein F3, CNTN1

1 Images

Project5620****

Western blot - Anti-Contactin 1 antibody (AB66265)

Contactin 1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-Contactin 1 antibody (ab66265) at 1 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 2:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 3:

Brain (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 113 kDa

Observed band size: 120 kDa

false

Exposure time: 8min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Contactin 1 also known as contactin-1 or contactin is a protein involved in cell adhesion processes. It is sometimes referred to as contacton. This protein has a molecular weight of approximately 120 kDa and is a neural membrane glycoprotein. Contactin 1 primarily expresses in the developing nervous system and persists in the adult central nervous system. It plays an important role in forming connections between neurons and ensuring proper communication between them.

Biological function summary

The role of this protein involves maintaining neuronal network organization and integrity. Contactin 1 plays a critical part in myelination a process essential for the swift conduction of nerve impulses. It interacts with several partners forming complexes essential for neuronal stability including neurofascin and contactin-associated protein-like 2. These interactions facilitate proper signal transmission across synapses in the nervous system.

Pathways

Contactin 1 is an important element in the pathways that regulate neuronal development and myelination. It is involved in the formation of the nodes of Ranvier important for nerve impulse transmission. Contactin 1 interacts with proteins such as tenascin-R and NrCAM in these processes contributing significantly to the formation and maintenance of functional neural networks.

Defects in the regulation or expression of contactin 1 are associated with neurological conditions like multiple sclerosis and Guillain-Barré syndrome. These disorders highlight problems in nerve signal transmission and immune system misregulation. Through these diseases contactin 1's interaction with proteins such as CASPR (contactin-associated protein-like 2) becomes important as disruptions in these interactions can lead to neuronal dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth (By similarity).

See full target information CNTN1

Additional targets

CNTN1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Experimental eye research 207:108553 PubMed33811915

2021

ON-bipolar cell gene expression during retinal degeneration: Implications for optogenetic visual restoration.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Gilhooley,Doron G Hickey,Moritz Lindner,Teele Palumaa,Steven Hughes,Stuart N Peirson,Robert E MacLaren,Mark W Hankins

Frontiers in molecular neuroscience 13:563091 PubMed33192291

2020

Isolation and Characterization of Multi-Protein Complexes Enriched in the K-Cl Co-transporter 2 From Brain Plasma Membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua L Smalley,Georgina Kontou,Catherine Choi,Qiu Ren,David Albrecht,Krithika Abiraman,Miguel A Rodriguez Santos,Christopher E Bope,Tarek Z Deeb,Paul A Davies,Nicholas J Brandon,Stephen J Moss

Biomolecules 10: PubMed32806791

2020

Contactin-1 Is Reduced in Cerebrospinal Fluid of Parkinson's Disease Patients and Is Present within Lewy Bodies.

Applications

Unspecified application

Species

Unspecified reactive species

Madhurima Chatterjee,Inger van Steenoven,Evelien Huisman,Linda Oosterveld,Henk Berendse,Wiesje M van der Flier,Marta Del Campo,Afina W Lemstra,Wilma D J van de Berg,Charlotte E Teunissen

Oncology letters 19:1611-1618 PubMed32002038

2020

Upregulation of contactin-1 expression promotes prostate cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Boren Wang,Xi Yang,Ting Zhao,Hanghang Du,Tong Wang,Suping Zhong,Bo Yang,Hui Li

EMBO molecular medicine 10: PubMed30266776

2018

Early auto-immune targeting of photoreceptor ribbon synapses in mouse models of multiple sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mayur Dembla,Ajay Kesharwani,Sivaraman Natarajan,Claudia Fecher-Trost,Richard Fairless,Sarah K Williams,Veit Flockerzi,Ricarda Diem,Karin Schwarz,Frank Schmitz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Contactin 1 antibody

Western blot - Anti-Contactin 1 antibody (AB66265)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (5)

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