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AB168243

Anti-COP antibody

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(1 Publication)

Mouse Polyclonal COP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CARD16.

View Alternative Names

COP, COP1, CARD16, Caspase recruitment domain-containing protein 16, Caspase recruitment domain-only protein 1, Caspase-1 inhibitor COP, Pseudo interleukin-1 beta converting enzyme, CARD-only protein 1, Pseudo-ICE, Pseudo-IL1B-converting enzyme

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-COP antibody (AB168243)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-COP antibody (AB168243)

Immunofluorescent analysis of permeabilized HeLa cells labeling COP with ab168243 at 10μg/ml.

Western blot - Anti-COP antibody (AB168243)
  • WB

Unknown

Western blot - Anti-COP antibody (AB168243)

All lanes:

Western blot - Anti-COP antibody (ab168243) at 1 µg/mL

Lane 1:

COP transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 23 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CARD16.

Q5EG05

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Complementary Products

Primary Antibodies

AB181224

Anti-alpha COP I/COPA antibody [EPR14273(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha COP I/COPA antibody [EPR14273(B)] (AB181224)

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Primary Antibodies

AB176594

Anti-ZZEF1 antibody

Immunoprecipitation - Anti-ZZEF1 antibody (AB176594)

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Primary Antibodies

AB2899

Anti-beta COP antibody

Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (AB2899)

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Primary Antibodies

AB273052

Anti-ZWINT antibody [EPR23440-96] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-ZWINT antibody [EPR23440-96] - BSA and Azide free (AB273052)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

COP also known as coat protein complex refers to a group of proteins involved in vesicle formation. These proteins are part of coatomer protein complexes like COPI and COPII which play roles in transport within cells. COP proteins have an approximate mass of 600-700 kDa depending on the specific complex. Inside the cell COP proteins are expressed in the Golgi apparatus and endoplasmic reticulum. In research the term 'COP mouse' and related terms like 'mouse COP' often emerge referring to studies in model organisms like mice to understand these proteins' functions better.
Biological function summary

COP proteins regulate intracellular transport by forming vesicles that shuttle materials between organelles. COPI is predominantly involved in retrograde transport from the Golgi to the endoplasmic reticulum while COPII handles anterograde transport from the endoplasmic reticulum to the Golgi. These coatomer complexes are vital components of the cellular sorting machinery affecting the flow of lipids and proteins within cells. The function of COP proteins influences cellular processes maintaining cellular organization and proper protein localization.

Pathways

COP proteins integrate into intracellular transport processes that impact various signaling pathways. COPI for example operates within the vesicular transport pathway contributing to the recycling of proteins. Notably COPII is important in the secretory pathway impacting the export of proteins from the endoplasmic reticulum. This activity involves coordination with other proteins like SNAREs which facilitate vesicle fusion and delivery of contents to target membranes.

Defective COP function links to certain conditions such as cranio-lenticulo-sutural dysplasia and congenital dyserythropoietic anemia. Dysfunction in COPII proteins including Sec23 and Sec24 associates with cranio-lenticulo-sutural dysplasia demonstrating its role in maintaining correct protein trafficking. Additionally mutations impacting COPI proteins tie to congenital dyserythropoietic anemia revealing the relevance of vesicle transport in blood cell formation. Understanding connections between COP proteins and these diseases aids in elucidating the cellular transport's role in human health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Caspase inhibitor. Acts as a regulator of procaspase-1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro-inflammatory cytokine response. Also able to inhibit CASP1-mediated neuronal cell death, TNF-, hypoxia-, UV-, and staurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspases CASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2.
See full target information CARD16
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Artificial cells, nanomedicine, and biotechnology 47:2048-2057 PubMed31126199

2019

K-Ras-PI3K/AKT pathway regulates H1.4 through PKA to promote the occurrence and development of osteosarcoma cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jingzhe Zhang,Wanguo Liu,Hang Dong,Wenjun Wang
View all publications
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Product promise

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