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Mouse Monoclonal COP1 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 15 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COP1 aa 600 to C-terminus.


Images

Western blot - Anti-COP1 antibody [1E4] (AB56400), expandable thumbnail
  • Flow Cytometry - Anti-COP1 antibody [1E4] (AB56400), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-COP1 antibody [1E4] (AB56400), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COP1 antibody [1E4] (AB56400), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human COP1 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NHY2

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-PICC/IF
Human
Expected
Expected
Tested
Expected
Mouse
Tested
Tested
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
-
Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable).

Alternative names

RFWD2, RNF200, COP1, E3 ubiquitin-protein ligase COP1, Constitutive photomorphogenesis protein 1 homolog, RING finger and WD repeat domain protein 2, RING finger protein 200, RING-type E3 ubiquitin transferase RFWD2, hCOP1

Recommended products

Mouse Monoclonal COP1 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 15 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COP1 aa 600 to C-terminus.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human COP1 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NHY2
Clone number
1E4
Purity
Tissue culture supernatant
Light chain type
kappa
Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 30th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

COP1 also known as RING finger and WD-40 repeat-containing protein 53 or RFWD2 is an E3 ubiquitin ligase weighing approximately 85 kDa. It primarily regulates protein turnover through ubiquitylation. COP1 localizes in the nucleus and cytoplasm and influences protein stability by marking target proteins for degradation. Its known targets include transcription factors and regulatory proteins. COP1's role extends across various organisms often expressed in multiple human tissues highlighting its importance in cellular regulation.

Biological function summary

COP1 affects multiple cellular processes including the control of photoperiodic flowering and circadian rhythm in plants. In mammals COP1 associates with other proteins as part of a larger complex that regulates gene expression. It modulates cell cycle progression and impacts tumor suppressor pathways. This protein complex interaction allows COP1 to target specific substrates for ubiquitylation impacting biological functions relevant to organismal development.

Pathways

COP1 participates in the light signaling pathway and the p53 signaling pathway. In the p53 pathway COP1 targets p53 an important tumor suppressor protein for degradation. This interaction can influence the pathway's regulation impacting the cell cycle and apoptosis. COP1's involvement in these pathways illustrates its multifunctional nature and its ability to respond to environmental cues by altering cellular states and responses.

Associated diseases and disorders

COP1 has been associated with cancer due to its regulatory role in the p53 tumor suppressing pathway. Abnormal expression or mutation of COP1 can lead to uncontrolled degradation of p53 increasing the risk of tumor development. Additionally alterations in COP1 have been implicated in metabolic disorders where it influences insulin signaling. Researchers continue to study COP1's interactions with other proteins like MDM2 another E3 ligase to better understand its role in disease modulation and potential therapeutic approaches.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-COP1 antibody [1E4] (ab56400), expandable thumbnail

    Western blot - Anti-COP1 antibody [1E4] (ab56400)

    COP1 antibody (ab56400) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-COP1 antibody [1E4] (ab56400)

    Predicted band size: 80 kDa

  • Flow Cytometry - Anti-COP1 antibody [1E4] (ab56400), expandable thumbnail

    Flow Cytometry - Anti-COP1 antibody [1E4] (ab56400)

    Overlay histogram showing NIH3T3 cells stained with ab56400 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56400, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (Mouse IgG2b [PLPV219] - Isotype Control ab91366, 2μg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-COP1 antibody [1E4] (ab56400), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-COP1 antibody [1E4] (ab56400)

    Immunofluorescence of ab56400 on NIH/3T3 cell [antibody concentration 10 ug/ml].

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COP1 antibody [1E4] (ab56400), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COP1 antibody [1E4] (ab56400)

    IHC image of COP1 staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56400, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This image was generated using the ascites version of the product.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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