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AB56400

Anti-COP1 antibody [1E4]

4

(4 Reviews)

|

(19 Publications)

Mouse Monoclonal COP1 antibody. Suitable for WB and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COP1 aa 600 to C-terminus.

View Alternative Names

RFWD2, RNF200, COP1, E3 ubiquitin-protein ligase COP1, Constitutive photomorphogenesis protein 1 homolog, RING finger and WD repeat domain protein 2, RING finger protein 200, RING-type E3 ubiquitin transferase RFWD2, hCOP1

2 Images
Western blot - Anti-COP1 antibody [1E4] (AB56400)
  • WB

Supplier Data

Western blot - Anti-COP1 antibody [1E4] (AB56400)

Blocking and diluting buffer and concentration : 5% NFDM/PBST.

Primary recommended usage : 1 ~ 5 µg/ml. Recommended loading amount : 25 ~ 50 µg/lane. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP Conjugate 1/2500 ~ 1/5000. This should be used only as a guide, each investigator should determine the optimal antigen amount and antibody dilution for their own specific research application.

All lanes:

Western blot - Anti-COP1 antibody [1E4] (ab56400)

All lanes:

K-562 (human immortalized myeloid leukemia cells) cell lysate

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate

Predicted band size: 80 kDa

Observed band size: 80 kDa

false

Western blot - Anti-COP1 antibody [1E4] (AB56400)
  • WB

Supplier Data

Western blot - Anti-COP1 antibody [1E4] (AB56400)

Blocking and diluting buffer and concentration : 5% NFDM/PBST.

Primary recommended usage : 1 ~ 5 µg/ml. Recommended loading amount : 25 ~ 50 µg/lane. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP Conjugate 1/2500 ~ 1/5000. This should be used only as a guide, each investigator should determine the optimal antigen amount and antibody dilution for their own specific research application.

All lanes:

Western blot - Anti-COP1 antibody [1E4] (ab56400)

All lanes:

Recombinant RFWD2

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate

Observed band size: 37 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1E4

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human COP1 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8NHY2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

This product was changed from ascites to tissue culture supernatant on 30th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Purification notes
Purified from TCS.
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

COP1 also known as RING finger and WD-40 repeat-containing protein 53 or RFWD2 is an E3 ubiquitin ligase weighing approximately 85 kDa. It primarily regulates protein turnover through ubiquitylation. COP1 localizes in the nucleus and cytoplasm and influences protein stability by marking target proteins for degradation. Its known targets include transcription factors and regulatory proteins. COP1's role extends across various organisms often expressed in multiple human tissues highlighting its importance in cellular regulation.
Biological function summary

COP1 affects multiple cellular processes including the control of photoperiodic flowering and circadian rhythm in plants. In mammals COP1 associates with other proteins as part of a larger complex that regulates gene expression. It modulates cell cycle progression and impacts tumor suppressor pathways. This protein complex interaction allows COP1 to target specific substrates for ubiquitylation impacting biological functions relevant to organismal development.

Pathways

COP1 participates in the light signaling pathway and the p53 signaling pathway. In the p53 pathway COP1 targets p53 an important tumor suppressor protein for degradation. This interaction can influence the pathway's regulation impacting the cell cycle and apoptosis. COP1's involvement in these pathways illustrates its multifunctional nature and its ability to respond to environmental cues by altering cellular states and responses.

COP1 has been associated with cancer due to its regulatory role in the p53 tumor suppressing pathway. Abnormal expression or mutation of COP1 can lead to uncontrolled degradation of p53 increasing the risk of tumor development. Additionally alterations in COP1 have been implicated in metabolic disorders where it influences insulin signaling. Researchers continue to study COP1's interactions with other proteins like MDM2 another E3 ligase to better understand its role in disease modulation and potential therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable).
See full target information COP1
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1570727 PubMed40395328

2025

COP1 drives renal cell carcinoma progression by targeting ACSL4 for ubiquitin-mediated degradation and inhibiting ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxiao Zheng,Lei Jiang,Feng Qi,Bo Peng

Journal of translational medicine 22:852 PubMed39304928

2024

STK40 inhibits trophoblast fusion by mediating COP1 ubiquitination to degrade P57.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Li,Li-Zhen Shao,Zhuo-Hang Li,Yong-Heng Wang,Qin-Yu Cai,Shun Wang,Hong Chen,Jie Sheng,Xin Luo,Xue-Mei Chen,Ying-Xiong Wang,Yu-Bin Ding,Tai-Hang Liu

Nature metabolism 3:1648-1661 PubMed34903883

2021

Peroxisomal β-oxidation acts as a sensor for intracellular fatty acids and regulates lipolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Lianggong Ding,Wenfei Sun,Miroslav Balaz,Anyuan He,Manuel Klug,Stefan Wieland,Robert Caiazzo,Violeta Raverdy,Francois Pattou,Philippe Lefebvre,Irfan J Lodhi,Bart Staels,Markus Heim,Christian Wolfrum

Oncogene 41:173-190 PubMed34716429

2021

The E3 ligase COP1 promotes ERα signaling and suppresses EMT in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Seng Chuan Tang,Quentin Lion,Olivier Peulen,Philippe Chariot,Arnaud Lavergne,Alice Mayer,Paula Allepuz Fuster,Pierre Close,Sebastian Klein,Alexandra Florin,Reinhard Büttner,Ivan Nemazanyy,Kateryna Shostak,Alain Chariot

Cell death discovery 7:196 PubMed34315867

2021

TRIB2 desensitizes ferroptosis via βTrCP-mediated TFRC ubiquitiantion in liver cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Susu Guo,Yuxin Chen,Xiangfei Xue,Yueyue Yang,Yikun Wang,Shiyu Qiu,Jiangtao Cui,Xiao Zhang,Lifang Ma,Yongxia Qiao,Jiayi Wang

Frontiers in cell and developmental biology 9:675939 PubMed33968945

2021

Targeting RFWD2 as an Effective Strategy to Inhibit Cellular Proliferation and Overcome Drug Resistance to Proteasome Inhibitor in Multiple Myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjie Guo,Pinggang Ding,Zhen Zhu,Lu Fan,Yanyan Zhou,Shu Yang,Ye Yang,Chunyan Gu

Communications biology 4:119 PubMed33500545

2021

Circulating extracellular vesicles release oncogenic miR-424 in experimental models and patients with aggressive prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Domenico Albino,Martina Falcione,Valeria Uboldi,Dada Oluwaseyi Temilola,Giada Sandrini,Jessica Merulla,Gianluca Civenni,Aleksandra Kokanovic,Alessandra Stürchler,Dheeraj Shinde,Mariangela Garofalo,Ricardo Pereira Mestre,Vera Constâncio,Martha Wium,Jacopo Burrello,Nicolò Baranzini,Annalisa Grimaldi,Jean-Philippe Theurillat,Daniela Bossi,Lucio Barile,Rui M Henrique,Carmen Jeronimo,Luiz Fernando Zerbini,Carlo V Catapano,Giuseppina M Carbone

Journal of experimental & clinical cancer research 39:238 PubMed33168024

2020

MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8 T lymphocytes in human diffuse large B cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Xu,Zhenchuan Liu,Lixin Lv,Ping Li,Bing Xiu,Wenbin Qian,Aibin Liang

Veterinary and comparative oncology 18:570-579 PubMed32072720

2020

Development of synthetic microRNA-214 showing enhanced cytotoxicity and RNase resistance for treatment of canine hemangiosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ryutaro Yoshikawa,Kazuki Heishima,Yoshihito Ueno,Miwa Kawade,Yusuke Maeda,Kyoko Yoshida,Mami Murakami,Hiroki Sakai,Yukihiro Akao,Takashi Mori

The EMBO journal 38: PubMed30692133

2019

The pseudokinase TRIB1 toggles an intramolecular switch to regulate COP1 nuclear export.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer E Kung,Natalia Jura
View all publications
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Product promise

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