AB70889

Anti-COP1 antibody

Name: Anti-COP1 antibody (ab70889) | Abcam
Brand: Abcam Limited
SKU: AB70889
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal COP1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human COP1.

View Alternative Names

RFWD2, RNF200, COP1, E3 ubiquitin-protein ligase COP1, Constitutive photomorphogenesis protein 1 homolog, RING finger and WD repeat domain protein 2, RING finger protein 200, RING-type E3 ubiquitin transferase RFWD2, hCOP1

2 Images

Unknown

Immunoprecipitation - Anti-COP1 antibody (AB70889)

HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded), with ab70889 at 1 µg/ml for WB, subsequent to IP at 3 µg/mg lysate.
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-COP1 antibody (ab70889)

Predicted band size: 80 kDa

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Unknown

Western blot - Anti-COP1 antibody (AB70889)

All lanes:

Western blot - Anti-COP1 antibody (ab70889) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 80 kDa

Observed band size: 80 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human COP1. The exact immunogen used to generate this antibody is proprietary information.

Q8NHY2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mL", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

COP1 also known as RING finger and WD-40 repeat-containing protein 53 or RFWD2 is an E3 ubiquitin ligase weighing approximately 85 kDa. It primarily regulates protein turnover through ubiquitylation. COP1 localizes in the nucleus and cytoplasm and influences protein stability by marking target proteins for degradation. Its known targets include transcription factors and regulatory proteins. COP1's role extends across various organisms often expressed in multiple human tissues highlighting its importance in cellular regulation.

Biological function summary

COP1 affects multiple cellular processes including the control of photoperiodic flowering and circadian rhythm in plants. In mammals COP1 associates with other proteins as part of a larger complex that regulates gene expression. It modulates cell cycle progression and impacts tumor suppressor pathways. This protein complex interaction allows COP1 to target specific substrates for ubiquitylation impacting biological functions relevant to organismal development.

Pathways

COP1 participates in the light signaling pathway and the p53 signaling pathway. In the p53 pathway COP1 targets p53 an important tumor suppressor protein for degradation. This interaction can influence the pathway's regulation impacting the cell cycle and apoptosis. COP1's involvement in these pathways illustrates its multifunctional nature and its ability to respond to environmental cues by altering cellular states and responses.

COP1 has been associated with cancer due to its regulatory role in the p53 tumor suppressing pathway. Abnormal expression or mutation of COP1 can lead to uncontrolled degradation of p53 increasing the risk of tumor development. Additionally alterations in COP1 have been implicated in metabolic disorders where it influences insulin signaling. Researchers continue to study COP1's interactions with other proteins like MDM2 another E3 ligase to better understand its role in disease modulation and potential therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable).

See full target information COP1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 15:3449-3459 PubMed40948525

2025

RNF200 enhances radiotherapy sensitivity by modulating PD-L1 stability in tumor-associated macrophages of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hongbo Xu,Feng Cai,Lu Xu,Dengsheng Jiang,Gengming Wang,Xianwen Zhang,Yajun Zhang

Frontiers in oncology 15:1581860 PubMed40406250

2025

Integrated analysis identifies P4HA2 as a key regulator of STAT1-mediated colorectal cancer progression and a potential biomarker for precision therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Qianshi Zhang,Yinan Zhang,Zhiwei Sun,Huanle Wang,Guohang Dai,Yue Meng,Shasha Shi,Shuangyi Ren

British journal of pharmacology 182:2662-2681 PubMed39993792

2025

Sculponeatin A induces mitochondrial dysfunction in non-small cell lung cancer through WWP2-mediated degradation of mitochondrial STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Wan,Chen Qian,Xuewen Liu,Yifan Zhong,Wenkang Peng,Liang Zhang,Xiangrong Zhan,Yongtong Huang,Chengyu Zhang,Jiu Wang,Yuan Si,Ying Liu

Experimental cell research 382:111428 PubMed31125554

2019

TRB3 stimulates SIRT1 degradation and induces insulin resistance by lipotoxicity via COP1.

Applications

Unspecified application

Species

Unspecified reactive species

Xingxing Ren,Ningxin Chen,Yawen Chen,Wei Liu,Yaomin Hu

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-COP1 antibody

Immunoprecipitation - Anti-COP1 antibody (AB70889)

Western blot - Anti-COP1 antibody (AB70889)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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