AB2915

Anti-COPB2 antibody

Name: Anti-COPB2 antibody (ab2915) | Abcam
Brand: Abcam Limited
SKU: AB2915

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(3 Publications)

Rabbit Polyclonal COPB2 antibody. Suitable for WB and reacts with Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Rat Copb1 aa 450-550.

View Alternative Names

Coatomer subunit beta', Beta'-coat protein, p102, Beta'-COP, Copb2

1 Images

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Western blot - Anti-COPB2 antibody (AB2915)

Western blot detection of beta COP I from PC-12 cell lysates using ab2915.

All lanes:

Western blot - Anti-COPB2 antibody (ab2915)

Predicted band size: 102 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

Synthetic Peptide within Rat Copb1 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

P23514

Specificity

Detects coatomer-protein I beta (COP I beta).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

COPB2 also known as Coatomer Subunit Beta 2 plays a vital role in intracellular protein transport. It is part of the coatomer protein complex which participates in vesicle formation during the transport between the Golgi apparatus and endoplasmic reticulum. This protein has a molecular mass of approximately 102 kDa and is widely expressed in various tissues with significant expression in the liver brain and lungs. Its presence in multiple tissues highlights its broad role in cellular functions.

Biological function summary

COPB2 functions as a component of the larger coatomer complex which is made up of several subunits. This complex facilitates the retrograde transport of proteins from the Golgi back to the endoplasmic reticulum. Through this process COPB2 helps maintain protein homeostasis and ensures that essential proteins are rerouted for proper cellular functioning. Its interaction with other proteins in the coatomer complex supports structural integrity and transport efficiency.

Pathways

COPB2 is integrally involved in the COPI vesicle-mediated transport pathway. This pathway is important for maintaining the secretory pathway's quality control and is interconnected with other pathways such as endocytosis. Related proteins within these pathways include ARF1 which regulates coat recruitment necessary for vesicle formation and other coatomer subunits that collectively influence vesicle trafficking and fusion events.

COPB2 has implications in neurodegenerative diseases and certain cancers. Its function in protein transport becomes critical in conditions where cellular transport mechanisms are disrupted. Abnormal levels or mutations of COPB2 have been associated with Alzheimer’s disease impacting protein aggregation and clearance. Additionally its interactions with proteins such as ARF1 and other coatomer subunits may influence cancer progression by affecting cellular growth and signaling pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).. This coatomer complex protein, essential for Golgi budding and vesicular trafficking, is a selective binding protein (RACK) for protein kinase C, epsilon type. It binds to Golgi membranes in a GTP-dependent manner.

See full target information Copb2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed38886055

2024

β-arrestins Are Scaffolding Proteins Required for Shh-Mediated Axon Guidance.

Applications

Unspecified application

Species

Unspecified reactive species

Rachelle Sauvé,Steves Morin,Patricia T Yam,Frédéric Charron

Experimental and therapeutic medicine 21:417 PubMed33747158

2021

Hyperresponsiveness of human gingival fibroblasts from patients with aggressive periodontitis against bacterial lipopolysaccharide.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Li,Xiaoxuan Wang,Qing-Xian Luan

Life (Basel, Switzerland) 11: PubMed33672637

2021

Spatio-Temporal Expression Pattern of Ki-67, pRB, MMP-9 and Bax in Human Secondary Palate Development.

Applications

Unspecified application

Species

Unspecified reactive species

Tanja Šimić Bilandžija,Katarina Vukojević,Anka Ćorić,Ivna Vuković Kekez,Ivana Medvedec Mikić,Lidija Lasić Arapović,Natalija Filipović,Jasminka Anđelić,Mirna Saraga-Babić,Danijela Kalibović Govorko

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-COPB2 antibody

Western blot - Anti-COPB2 antibody (AB2915)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise