Anti-COPB2 antibody - C-terminal

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPB2 antibody - C-terminal (AB192924)

Immunohistochemistry of human prostate carcinoma using ab192924 at 1/1000.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPB2 antibody - C-terminal (AB192924)

Immunohistochemistry of mouse renal cell carcinoma using ab192924 at 1/1000.

• WB

Supplier Data

Western blot - Anti-COPB2 antibody - C-terminal (AB192924)

All lanes:

Western blot - Anti-CD44 antibody [BLR038F] (<a href='/en-us/products/primary-antibodies/cd44-antibody-blr038f-ab243894'>ab243894</a>) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 10 µg

Lane 2:

786-O whole cell lysate at 10 µg

Lane 3:

HEK293T whole cell lysate at 10 µg

Lane 4:

A-549 whole cell lysate at 10 µg

Lane 5:

KG-1 cells whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 81 kDa

Observed band size: 90 kDa

true

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-COPB2 antibody - C-terminal (AB192924)

All lanes:

Western blot - Anti-Brd4 antibody [BL-149-2H5] (<a href='/en-us/products/primary-antibodies/brd4-antibody-bl-149-2h5-ab243862'>ab243862</a>) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 25 µg

Lane 2:

HEK293T whole cell lysate at 25 µg

Lane 3:

Jurkat whole cell lysate at 25 µg

Lane 4:

A-549 whole cell lysate at 25 µg

Lane 5:

SW620 whole cell lysate at 25 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 152 kDa

Observed band size: 210 kDa

true

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-COPB2 antibody - C-terminal (AB192924)

All lanes:

Western blot - Anti-Cdk7 antibody [BL-80-3D4] (<a href='/en-us/products/primary-antibodies/cdk7-antibody-bl-80-3d4-ab243863'>ab243863</a>) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 15 µg

Lane 2:

Jurkat whole cell lysate at 15 µg

Lane 3:

U2OS whole cell lysate at 15 µg

Lane 4:

LNCaP whole cell lysate at 15 µg

Lane 5:

RKO whole cell lysate at 15 µg

Lane 6:

HEK293T whole cell lysate at 15 µg

Lane 7:

MCF-7 whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 39 kDa

Observed band size: 39 kDa

true

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-COPB2 antibody - C-terminal (AB192924)

All lanes:

Western blot - Anti-PBRM1/BAF180 antibody [BL-39-2C3] (<a href='/en-us/products/primary-antibodies/pbrm1-baf180-antibody-bl-39-2c3-ab243876'>ab243876</a>) at 1/1000 dilution

Lane 1:

BW5147.3 whole cell lysate at 50 µg

Lane 2:

EL4 whole cell lysate at 50 µg

Lane 3:

mIMCD-3 whole cell lysate at 50 µg

Lane 4:

RenCa whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Lane 6:

TCMK-1 whole cell lysate at 50 µg

Lane 7:

C2C12 whole cell lysate at 50 µg

Lane 8:

A20 whole cell lysate at 50 µg

Lane 9:

Ba/F3 whole cell lysate at 50 µg

Lane 10:

CH27 whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 187 kDa

Observed band size: 200 kDa

true

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-COPB2 antibody - C-terminal (AB192924)

All lanes:

Western blot - Anti-Brd4 antibody [BL-149-2H5] (<a href='/en-us/products/primary-antibodies/brd4-antibody-bl-149-2h5-ab243862'>ab243862</a>) at 1/1000 dilution

Lane 1:

NIH 3T3 whole cell lysate at 10 µg

Lane 2:

CT26 whole cell lysate at 10 µg

Lane 3:

CH27 whole cell lysate at 10 µg

Lane 4:

TCMK-1 whole cell lysate at 10 µg

Lane 5:

BW5147.3 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 155 kDa

Observed band size: 210 kDa

true

Exposure time: 10s

• IP

Supplier Data

Immunoprecipitation - Anti-COPB2 antibody - C-terminal (AB192924)

Detection of COPB2 by Western Blot of Immunprecipitate.
Lane 1 : ab192924 at 0.1 μg/ml labeling COPB2 in 293T whole cell lysate (prepared using NETN lysis buffer) immunoprecipitated using ab192924 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2 : Control IgG.
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-COPB2 antibody - C-terminal (ab192924)

Predicted band size: 102 kDa

false

• WB

Supplier Data

Western blot - Anti-COPB2 antibody - C-terminal (AB192924)

Lysates were prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-COPB2 antibody - C-terminal (ab192924) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK1 whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 102 kDa

true

Exposure time: 30s