AB180946

Anti-COPE antibody [EPR12579]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal COPE antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Coatomer subunit epsilon, Epsilon-coat protein, Epsilon-COP, COPE

4 Images

Flow Cytometry (Intracellular) - Anti-COPE antibody [EPR12579] (AB180946)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-COPE antibody [EPR12579] (AB180946)

Intracellular Flow Cytometry analysis of A375 cells labeling COPE using ab180946 at a 1/70 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

Immunocytochemistry/ Immunofluorescence - Anti-COPE antibody [EPR12579] (AB180946)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-COPE antibody [EPR12579] (AB180946)

Immunofluorescent analysis of formaldehyde fixed MCF-7 cells labeling COPE using ab180946 at a 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) was used as the secondary at a 1/200 dilution. Counterstain DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPE antibody [EPR12579] (AB180946)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPE antibody [EPR12579] (AB180946)

Immunohistochemical analysis of paraffin embedded Human liver tissue sections labeling COPE using ab180946 at a 1/50 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-COPE antibody [EPR12579] (AB180946)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-COPE antibody [EPR12579] (ab180946) at 1/5000 dilution

Lane 1:

MCF-7 cell lysate at 20 µg

Lane 2:

PC-3 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

A375 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa

false

Carrier free

Anti-COPE antibody [EPR12579] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12579

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/70", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The COPE protein also known as Coatomer Protein Complex Subunit E is a component of the COPI coatomer complex. This protein roughly weighs approximately 34 kDa and is present in the Golgi apparatus where it takes part in vesicle formation processes. COPE is expressed in numerous cell types highlighting its importance in intracellular transport mechanisms and maintaining cellular organization.

Biological function summary

COPE plays a significant role in the retrograde transport of proteins from the Golgi back to the endoplasmic reticulum being part of the larger COPI complex. The COPI coatomer complex operates in conjunction with other coat proteins like clathrin to ensure vesicle trafficking and membrane dynamics. This mechanism is essential for membrane recycling protein sorting and other cellular logistics.

Pathways

COPE is important in the secretory pathway. It interacts closely with other components like ARF1 a small GTPase that regulates coat assembly and disassembly to maintain efficient protein sorting. Moreover COPE's role intersects with the ER-to-Golgi transport pathway acting alongside proteins like Sec27 demonstrating its connection within a tightly regulated trafficking network essential for cellular function.

COPE’s malfunction associates with neurodegenerative diseases. Disruption in its role in vesicular trafficking can lead to protein aggregation disorders like Alzheimer's disease where protein accumulation becomes pathogenic. COPE interaction with proteins such as APP (Amyloid Precursor Protein) becomes evident in the pathology of Alzheimer's showcasing its importance in disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated with ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).

See full target information Coatomer subunit epsilon

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

NAR cancer 6:zcae015 PubMed38596432

2024

The DNA Damage Response (DDR) landscape of endometrial cancer defines discrete disease subtypes and reveals therapeutic opportunities.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyuan Zhang,Sayali Joseph,Di Wu,Jessica L Bowser,Cyrus Vaziri

Journal of virology 96:e0219321 PubMed35044210

2022

ARF1 with Sec7 Domain-Dependent GBF1 Activates Coatomer Protein I To Support Classical Swine Fever Virus Entry.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhang,Tao Wang,Yanyan Yi,Mengzhao Song,Mingxing Jin,Kangkang Guo,Yanming Zhang

Methods in enzymology 622:309-328 PubMed31155059

2019

Optogenetic perturbation of the biochemical pathways that control cell behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren L Haar,David S Lawrence,Robert M Hughes

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-COPE antibody [EPR12579]

Flow Cytometry (Intracellular) - Anti-COPE antibody [EPR12579] (AB180946)

Immunocytochemistry/ Immunofluorescence - Anti-COPE antibody [EPR12579] (AB180946)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPE antibody [EPR12579] (AB180946)

Western blot - Anti-COPE antibody [EPR12579] (AB180946)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise