AB229807

Anti-COPS3/CSN3 antibody

Name: Anti-COPS3/CSN3 antibody (ab229807) | Abcam
Brand: Abcam Limited
SKU: AB229807

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(3 Publications)

Rabbit Polyclonal COPS3/CSN3 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COPS3 aa 150 to C-terminus.

View Alternative Names

CSN3, COPS3, COP9 signalosome complex subunit 3, SGN3, Signalosome subunit 3, JAB1-containing signalosome subunit 3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPS3/CSN3 antibody (AB229807)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPS3/CSN3 antibody (AB229807)

Paraffin-embedded human heart tissue stained for COPS3/CSN3 using ab229807 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPS3/CSN3 antibody (AB229807)

Paraffin-embedded human testis tissue stained for COPS3/CSN3 using ab229807 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Immunoprecipitation - Anti-COPS3/CSN3 antibody (AB229807)

COPS3/CSN3 was immunoprecipitated from 500 μg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 μg ab229807.

Lane 1 : Rabbit monoclonal IgG IP in HeLa whole cell lysate.

Lane 2 : ab229807 IP in HeLa whole cell lysate.

Lane 3 : HeLa whole cell lysate 20 μg.

For western blotting, an HRP-conjugated anti-rabbit IgG,specific to the non-reduced form of IgG was used as the secondary antibody at 1/50000 dilution.

All lanes:

Immunoprecipitation - Anti-COPS3/CSN3 antibody (ab229807)

Predicted band size: 48 kDa

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Supplier Data

Western blot - Anti-COPS3/CSN3 antibody (AB229807)

All lanes:

Western blot - Anti-COPS3/CSN3 antibody (ab229807) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 4:

HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate

Lane 5:

Mouse heart lysate

Lane 6:

Mouse brain lysate

Lane 7:

Mouse ovarian lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 48 kDa

Observed band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human COPS3 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UNS2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

COPS3 also known as CSN3 or COP9 forms a component of the COP9 signalosome complex. It weighs around 53 kDa. COPS3 is mainly expressed in the cytoplasm of cells in various tissues including the brain heart and skeletal muscle. As part of the signalosome COPS3 plays a role in cellular and molecular processes by influencing protein levels through deneddylation.

Biological function summary

The COPS3/CSN3 protein functions as part of the COP9 signalosome complex which acts in regulating the ubiquitin-proteasome pathway. This complex is involved in mediating the stability and degradation of key regulatory proteins by removing NEDD8 from cullin-RING ligases. By modulating these ligases COPS3 affects protein turnover and signaling pathways essential for cell cycle control and DNA damage response.

Pathways

COPS3/CSN3 takes part in the ubiquitin-proteasome pathway and the DNA damage response pathway. By regulating cullin-RING E3 ubiquitin ligases it influences the degradation of proteins involved in these pathways like p27 and CDT1. Interacting with proteins such as CUL1 and CUL3 COPS3 assists in maintaining cellular homeostasis by ensuring timely protein degradation.

Defects or dysregulation of COPS3 have links to cancer and neurodegenerative diseases. Its role in cancer involves connection to known proteins like p27 where altered protein turnover can lead to unchecked cell proliferation. In neurodegenerative diseases the connection lies with the UPS (ubiquitin-proteasome system) dysfunction affecting protein aggregation processes seen in disorders like Parkinson's disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.

See full target information COPS3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:4237 PubMed38762492

2024

Hsc70 promotes anti-tumor immunity by targeting PD-L1 for lysosomal degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Xu,Tingxue Xie,Mengxin Zhou,Yaqin Sun,Fengqi Wang,Yanan Tian,Ziyan Chen,Yanqi Xie,Ronghai Wu,Xufeng Cen,Jichun Zhou,Tingjun Hou,Lei Zhang,Chaoyang Huang,Qingwei Zhao,Dongrui Wang,Hongguang Xia

Autophagy 19:1693-1710 PubMed36451342

2022

The COPS3-FOXO3 positive feedback loop regulates autophagy to promote cisplatin resistance in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jianfang Niu,Taiqiang Yan,Wei Guo,Wei Wang,Tingting Ren,Yi Huang,Zhiqing Zhao,Yiyang Yu,Chenglong Chen,Qingshan Huang,Jingbing Lou,Lei Guo

Neuropsychiatric disease and treatment 17:1217-1227 PubMed33911869

2021

COP9 Signalosome Subunit 3 Restricts Neuroinflammatory Responses During Cerebral Ischemia/Reperfusion Injury Through Stabilizing Suppressor of Cytokine Signaling 3 Protein.

Applications

Unspecified application

Species

Unspecified reactive species

En Liang,Xiaojun Li,Wenjun Fu,Changtong Zhao,Baoying Yang,Zhonghua Yang

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-COPS3/CSN3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPS3/CSN3 antibody (AB229807)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPS3/CSN3 antibody (AB229807)

Immunoprecipitation - Anti-COPS3/CSN3 antibody (AB229807)

Western blot - Anti-COPS3/CSN3 antibody (AB229807)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Multiplex Kits

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise