Anti-COPS3/CSN3 antibody [EPR3127] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-COPS3/CSN3 antibody [EPR3127] - BSA and Azide free (AB247508)

This data was developed using ab79698, the same antibody clone in a different buffer formulation.

Immunofluorescence staining of HeLa cells with purified ab79698 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor^® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-COPS3/CSN3 antibody [EPR3127] - BSA and Azide free (AB247508)

This data was developed using ab79698, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab79698 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79698, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• WB

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Western blot - Anti-COPS3/CSN3 antibody [EPR3127] - BSA and Azide free (AB247508)

This data was developed using ab79698, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-COPS3/CSN3 antibody [EPR3127] (<a href='/en-us/products/primary-antibodies/cops3-csn3-antibody-epr3127-ab79698'>ab79698</a>) at 1/20000 dilution

Lane 1:

HT-29 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

SKBR-3 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

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