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AB250687

Anti-CoREST antibody [EPR13825] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CoREST antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Rat samples. Cited in 1 publication.

View Alternative Names

KIAA0071, RCOR, RCOR1, REST corepressor 1, Protein CoREST

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-CoREST antibody [EPR13825] - BSA and Azide free (AB250687)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CoREST antibody [EPR13825] - BSA and Azide free (AB250687)

This data was developed using ab183711, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of acetone-fixed HeLa cells labeling CoREST with ab183711 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (green). Counter stained with Dapi (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CoREST antibody [EPR13825] - BSA and Azide free (AB250687)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CoREST antibody [EPR13825] - BSA and Azide free (AB250687)

This data was developed using ab183711, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling CoREST with ab183711 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CoREST antibody [EPR13825] - BSA and Azide free (AB250687)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CoREST antibody [EPR13825] - BSA and Azide free (AB250687)

This data was developed using ab183711, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling CoREST with ab183711 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-CoREST antibody [EPR13825] - BSA and Azide free (AB250687)
  • WB

Supplier Data

Western blot - Anti-CoREST antibody [EPR13825] - BSA and Azide free (AB250687)

This data was developed using ab183711, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CoREST antibody [EPR13825] (<a href='/en-us/products/primary-antibodies/corest-antibody-epr13825-ab183711'>ab183711</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

MOLT-4 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa

Observed band size: 66 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13825

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250687 is the carrier-free version of ab183711.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CoREST protein also known as REST corepressor 1 is a 58 kDa protein that mechanically functions in chromatin remodeling and gene silencing. CoREST localizes to the nucleus and functions in the repression of neuronal genes in non-neuronal tissues. This protein is pivotal in assembling histone deacetylase complexes which makes it essential in epigenetic regulation. CoREST is expressed in various tissues throughout the body playing an important role in maintaining non-neuronal gene expression.
Biological function summary

CoREST interacts with multiple partners to form a corepressor complex. This complex includes LSD1 (lysine-specific demethylase 1) and HDAC1/2 (histone deacetylases 1 or 2) facilitating transcriptional repression. CoREST regulates the expression of genes involved in neurogenesis and cell differentiation processes. Its role is critical in maintaining the balance between neuronal and non-neuronal gene expression by modifying chromatin states.

Pathways

CoREST is involved in histone modification and gene expression regulation pathways. One significant pathway is the REST (RE1-Silencing Transcription factor) pathway where CoREST serves as a core component. In this pathway CoREST interacts with REST a transcriptional repressor to silence neural genes in a non-neuronal context. Another involved pathway is the chromatin remodeling pathway where CoREST associates with LSD1 and other chromatin-modifying enzymes to alter chromatin structure and gene accessibility.

CoREST has connections to neurological disorders and cancer. Altered CoREST expression or function links to the progression of neurodegenerative diseases such as Huntington's disease. CoREST dysregulation also relates to certain cancer types where misexpression can contribute to tumorigenesis. In the context of these disorders CoREST often collaborates with LSD1 affecting the transcriptional landscape of affected cells showcasing the importance of CoREST in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation.
See full target information RCOR1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 5: PubMed31094704

2019

Protective role of B cells in sterile particulate-induced lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shaikh M Atif,Douglas G Mack,Amy S McKee,Javier Rangel-Moreno,Allison K Martin,Andrew Getahun,Lisa A Maier,John C Cambier,Rubin Tuder,Andrew P Fontenot
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com