Rabbit Polyclonal CoREST2 / RCOR2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications.
Images
Publications
Filter by
- The Journal of biological chemistry 299:1046622023The stem cell-supporting small molecule UM171 triggers Cul3-KBTBD4-mediated degradation of ELM2 domain-harboring proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKristijonas Žemaitis,Sudip Ghosh,Jenny Hansson,Agatheeswaran SubramaniamPubMed 36997086
- Disease models & mechanisms 14:2021Sox11 regulates mammary tumour-initiating and metastatic capacity in Brca1-deficient mouse mammary tumour cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiu Man Tsang,Hyojin Kim,Erik Oliemuller,Richard Newman,Naa-Anyima Boateng,Naomi Guppy,Beatrice A HowardPubMed 33969421
- eLife 9:2020SOX11 promotes epithelial/mesenchymal hybrid state and alters tropism of invasive breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErik Oliemuller et. al.PubMed 32909943
- The Journal of clinical investigation 130:1830-18422020Inhibiting the coregulator CoREST impairs Foxp3+ Treg function and promotes antitumor immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Xiong,Liqing Wang,Eros Di Giorgio,Tatiana Akimova,Ulf H Beier,Rongxiang Han,Matteo Trevisanut,Jay H Kalin,Philip A Cole,Wayne W HancockPubMed 31917688
- Nucleic acids research 42:1656-702013RNA-dependent dynamic histone acetylation regulates MCL1 alternative splicing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDilshad H Khan et. al.PubMed 24234443
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Target data
Function
May act as a component of a corepressor complex that represses transcription.
Alternative names
REST corepressor 2, RCOR2
Recommended products
Rabbit Polyclonal CoREST2 / RCOR2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CoREST2 also known as RCOR2 is a corepressor protein weighing approximately 58 kDa. Mechanically CoREST2 functions by interacting with transcription factors and chromatin. It participates in remodeling chromatin structure which affects gene expression regulation. CoREST2 is primarily expressed in the brain but can be found in other tissues. Its interactions occur through its domains allowing it to bind with other proteins and DNA.
- Biological function summary
CoREST2 contributes to transcriptional repression and gene silencing. CoREST2 frequently forms part of larger protein complexes including the CoREST complex involving LSD1 and HDACs. This complex modulates chromatin to suppress specific genes particularly those associated with neuron differentiation and development. CoREST2 supports maintaining the silenced state of various genes participating in controlling cellular functions and fate.
- Pathways
Multiple processes rely on CoREST2 for maintaining gene expression regulation especially in neural pathways. It participates in pathways associated with neural differentiation and development. Through its association with the REST complex CoREST2 modulates neuronal genes alongside proteins like REST and LSD1. These pathways control how stem cells develop into neurons and maintain neural populations important for brain function.
- Associated diseases and disorders
CoREST2 has links to neurological conditions such as neurodevelopmental disorders and epilepsy. Its role in gene silencing connects CoREST2 to disorders resulting from disrupted gene regulation. In these contexts CoREST2 dysregulation might affect the balance of genes associated with developmental processes alongside proteins like HDAC2 contributing to disease development. Understanding CoREST2's functions could offer insights into therapeutic approaches for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-CoREST2 / RCOR2 antibody (ab37113)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab37113 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody (Goat Anti-Rabbit IgG H&L (HRP) ab6721), and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-CoREST2 / RCOR2 antibody (ab37113) at 1 µg/mL
Lane 1: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 25 µg
Lane 2: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 25 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 60 kDa
Exposure time: 4min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CoREST2 / RCOR2 antibody (ab37113)
IHC image of CoREST/RCOR2 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37113, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This image is courtesy of an anonymous customer review.Western blot - Anti-CoREST2 / RCOR2 antibody (ab37113)
All lanes: Western blot - Anti-CoREST2 / RCOR2 antibody (ab37113) at 1/1000 dilution
All lanes: MCF7 cells
SecondaryAll lanes: HRP conjugated donkey anti-rabbit at 1/2000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 45 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com