Anti-CoREST2 / RCOR2 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CoREST2 / RCOR2 antibody (AB37113)

IHC image of CoREST/RCOR2 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37113, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

AbReview29854****

Western blot - Anti-CoREST2 / RCOR2 antibody (AB37113)

All lanes:

Western blot - Anti-CoREST2 / RCOR2 antibody (ab37113) at 1/1000 dilution

All lanes:

MCF7 cells

Secondary

All lanes:

HRP conjugated donkey anti-rabbit at 1/2000 dilution.

Predicted band size: 58 kDa

Observed band size: 45 kDa

true

This image is courtesy of an anonymous abreview.

• WB

Lab

Western blot - Anti-CoREST2 / RCOR2 antibody (AB37113)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab37113 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody (ab6721), and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-CoREST2 / RCOR2 antibody (ab37113) at 1 µg/mL

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 25 µg

Lane 2:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 25 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

Predicted band size: 58 kDa

Observed band size: 60 kDa

false

Exposure time: 4min