Skip to main content

Rabbit Polyclonal CoREST3 / RCOR3 antibody. Suitable for ICC, IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human RCOR3.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CoREST3 / RCOR3 antibody (AB76921), expandable thumbnail
  • Immunocytochemistry - Anti-CoREST3 / RCOR3 antibody (AB76921), expandable thumbnail
  • Immunoprecipitation - Anti-CoREST3 / RCOR3 antibody (AB76921), expandable thumbnail
  • Western blot - Anti-CoREST3 / RCOR3 antibody (AB76921), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human RCOR3. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P2K3

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCIPWBIHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Expected
Chimpanzee
Predicted
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Elephant
Predicted
Predicted
Predicted
Predicted
Gorilla
Predicted
Predicted
Predicted
Predicted
Guinea pig
Predicted
Predicted
Predicted
Predicted
Horse
Predicted
Predicted
Predicted
Predicted
Orangutan
Predicted
Predicted
Predicted
Predicted
Rhesus monkey
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

2.00000-5.00000 µg/mg of lysate

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000.00000 - 1/10000.00000

Notes

-

Species

Mouse

Dilution info

1/2000.00000 - 1/10000.00000

Notes

-

Predicted
Predicted

Species

Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant

Dilution info

-

Notes

-

Target data

Function

May act as a component of a corepressor complex that represses transcription.

Alternative names

Recommended products

Rabbit Polyclonal CoREST3 / RCOR3 antibody. Suitable for ICC, IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human RCOR3.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human RCOR3. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P2K3
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CoREST3 also known as RCOR3 is a protein encoded by the RCOR3 gene. It functions as a corepressor involved in neuronal gene silencing. CoREST3 has a mass of approximately 54 kDa. It is expressed in various tissues with higher levels in the brain suggesting important roles in the central nervous system. Mechanically CoREST3 interacts with histone deacetylase enzymes and DNA-binding factors to modulate chromatin structure thereby influencing transcriptional repression.

Biological function summary

CoREST3 acts as a member of the CoREST corepressor complex. This complex plays significant roles in the regulation of gene expression by creating an epigenetically repressive environment. CoREST3 recruits other proteins such as LSD1 which demethylates histones resulting in the suppression of transcriptional activity. It maintains the silenced state of neuronal genes during development and differentiation contributing to neural functions and identity.

Pathways

CoREST3 participates in the REST (RE1-Silencing Transcription factor) pathway which is important for the repression of neuron-specific genes in non-neuronal cells. It works closely with the REST protein contributing to chromatin remodeling and gene silencing processes. Another essential pathway involving CoREST3 is the LSD1-mediated histone demethylation pathway. Through these pathways CoREST3 connects to proteins like REST and LSD1 to fulfill its role in transcriptional regulation.

Associated diseases and disorders

CoREST3's function in gene silencing links to neurological conditions such as Huntington's disease and schizophrenia. Altered CoREST3 expression or mutations can disrupt normal transcriptional repression leading to these disease states. In Huntington's disease changes in CoREST3 are associated with the misregulation of neuron-specific genes aligning its activity with that of REST and LSD1 in pathological progression. In schizophrenia RCOR3 is investigated for its role in gene regulation disturbances considering its interactions with the REST corepressor complex.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CoREST3 / RCOR3 antibody (ab76921), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CoREST3 / RCOR3 antibody (ab76921)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling CoREST3 / RCOR3 with ab76921 at 1/200 dilution.

  • Immunocytochemistry - Anti-CoREST3 / RCOR3 antibody (ab76921), expandable thumbnail

    Immunocytochemistry - Anti-CoREST3 / RCOR3 antibody (ab76921)

    Immunocytochemistry/Immunofluorescence analysis of NBF-fixed HeLa cells labelling CoREST3 / RCOR3 with ab76921 at 1/80 (2.5µg/ml). A DyLight® 488-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.

  • Immunoprecipitation - Anti-CoREST3 / RCOR3 antibody (ab76921), expandable thumbnail

    Immunoprecipitation - Anti-CoREST3 / RCOR3 antibody (ab76921)

    Detection of CoREST3 / RCOR3 by Western Blot of Immunprecipitate.

    ab76921 at 1µg/ml staining CoREST3 / RCOR3 in HeLa whole cell lysate immunoprecipitated using ab76921 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

    All lanes: Immunoprecipitation - Anti-CoREST3 / RCOR3 antibody (ab76921)

    Predicted band size: 56 kDa

  • Western blot - Anti-CoREST3 / RCOR3 antibody (ab76921), expandable thumbnail

    Western blot - Anti-CoREST3 / RCOR3 antibody (ab76921)

    All lanes: Western blot - Anti-CoREST3 / RCOR3 antibody (ab76921) at 0.04 µg/mL

    Lane 1: HeLa whole cell lysate at 50 µg

    Lane 2: HeLa whole cell lysate at 15 µg

    Lane 3: HeLa whole cell lysate at 5 µg

    Lane 4: 293T whole cell lysate at 50 µg

    Lane 5: NIH3T3 whole cell lysate at 50 µg

    Predicted band size: 56 kDa

    Observed band size: 70 kDa

    Exposure time: 1s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com