AB255812

Anti-Corin antibody [EPR22613-21]

BOND RX™ Validated
RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal CORIN antibody. Suitable for IHC-Fr, IHC-P and reacts with Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

Crn, Lrp4, Corin, Atrial natriuretic peptide-converting enzyme, Low density lipoprotein receptor-related protein 4, Pro-ANP-converting enzyme

4 Images

Immunohistochemistry (Frozen sections) - Anti-Corin antibody [EPR22613-21] (AB255812)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Corin antibody [EPR22613-21] (AB255812)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling Corin with ab255812 at 1/100 (5.47 μg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Corin antibody [EPR22613-21] (AB255812)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat kidney tissue labeling Corin with ab255812 at 1/100 (5.47 μg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR22613-21] (AB255812)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR22613-21] (AB255812)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Corin with ab255812 at 1/5000 dilution (0.11 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse kidney (PMID : 23327554, 27343265) is observed. The section was incubated with ab255812 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR22613-21] (AB255812)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Corin with ab255812 at 1 : /5000 dilution (0.11 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat kidney (PMID : 23327554, 27343265) is observed. The section was incubated with ab255812 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Carrier free

Anti-Corin antibody [EPR22613-21] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22613-21

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine-type endopeptidase involved in atrial natriuretic peptide (NPPA) processing (PubMed : 11884416, PubMed : 15637153). Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation (PubMed : 11884416, PubMed : 15637153, PubMed : 22418978). Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus (PubMed : 22437503). Also acts as a regulator of sodium reabsorption in kidney (PubMed : 20613715, PubMed : 22418978). May also process pro-NPPB the B-type natriuretic peptide (By similarity).

See full target information Corin

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:108137 PubMed39730060

2024

DNMT3b-mediated CpA methylation facilitates REST binding and gene silencing and exacerbates hippocampal demyelination in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tie-Feng Yao,Zhi-Yun Wang,Lu Sun,Sheng-Xue Yu,Hong-Dan Yu,Zheng-Zhong Yang,Wan-Ze Li,Lin Niu,Die Sun,Ya-Hui Shi,Jun-Qi Li,Wen-Qiang Liu,Xue-Zheng Liu,Zhong-Fu Zuo

Communications biology 7:1380 PubMed39443661

2024

Deficiencies in corin and atrial natriuretic peptide-mediated signaling impair endochondral ossification in bone development.

Applications

Unspecified application

Species

Unspecified reactive species

Zibin Zhou,Xiaoyu Mao,Chun Jiang,Wenguo Li,Tiantian Zhou,Meng Liu,Shijin Sun,Mengting Wang,Ningzheng Dong,Qingyu Wu,Haibin Zhou

Biology 12: PubMed37508377

2023

Corin Deficiency Diminishes Intestinal Sodium Excretion in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiabing Gu,Kun Wang,Wenguo Li,Meiling He,Tiantian Zhou,Meng Liu,Qingyu Wu,Ningzheng Dong

Frontiers in veterinary science 10:1127501 PubMed36923053

2023

Defining ovine dermal papilla cell markers and identifying key signaling pathways regulating its intrinsic properties.

Applications

Unspecified application

Species

Unspecified reactive species

Shanhe Wang,Tingyan Hu,Mingliang He,Yifei Gu,Xiukai Cao,Zehu Yuan,Xiaoyang Lv,Tesfaye Getachew,Kai Quan,Wei Sun

International journal of molecular sciences 23: PubMed36232551

2022

Renal Corin Is Essential for Normal Blood Pressure and Sodium Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Tiantian Zhou,Shengnan Zhang,Chunyu Du,Kun Wang,Xiabing Gu,Shijin Sun,Xianrui Zhang,Yayan Niu,Can Wang,Meng Liu,Ningzheng Dong,Qingyu Wu

Frontiers in cell and developmental biology 9:800157 PubMed34993204

2021

Single-Cell Transcriptomics Reveals the Molecular Anatomy of Sheep Hair Follicle Heterogeneity and Wool Curvature.

Applications

Unspecified application

Species

Unspecified reactive species

Shanhe Wang,Tianyi Wu,Jingyi Sun,Yue Li,Zehu Yuan,Wei Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Corin antibody [EPR22613-21]

Immunohistochemistry (Frozen sections) - Anti-Corin antibody [EPR22613-21] (AB255812)

Immunohistochemistry (Frozen sections) - Anti-Corin antibody [EPR22613-21] (AB255812)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR22613-21] (AB255812)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR22613-21] (AB255812)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

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