Rabbit Recombinant Monoclonal CORIN antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (AB274320), expandable thumbnail](https://content.abcam.com/products/images/corin-antibody-epr23361-77-ab274320--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17952.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (AB274320)")
![Western blot - Anti-Corin antibody [EPR23361-77] (AB274320), expandable thumbnail](https://content.abcam.com/products/images/corin-antibody-epr23361-77-ab274320--western-blot-img579.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Corin antibody [EPR23361-77] (AB274320)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (AB274320), expandable thumbnail](https://content.abcam.com/products/images/corin-antibody-epr23361-77-ab274320--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115867.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (AB274320)")
![Western blot - Anti-Corin antibody [EPR23361-77] (AB274320), expandable thumbnail](https://content.abcam.com/products/images/corin-antibody-epr23361-77-ab274320--western-blot-img4925.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Corin antibody [EPR23361-77] (AB274320)")
Publications
Filter by
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e227792023ANP promotes HTR-8/SVneo cell invasion by upregulating protein kinase N 3 via autophagy inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNan Chu,Yao Tang,Cheng-Jie Wang,Jiang-Nan Pei,Shou-Ling Luo,Yi Yu,Zhen-Zhen Liu,Hai-Yan Liu,Xue-Min Qiu,Ling Wang,Da-Jin Li,Wei-Rong GuPubMed 36723798
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Serine-type endopeptidase involved in atrial natriuretic peptide (NPPA) and brain natriuretic peptide (NPPB) processing (PubMed:10880574, PubMed:20489134, PubMed:21288900, PubMed:21763278). Converts through proteolytic cleavage the non-functional propeptides NPPA and NPPB into their active hormones, ANP and BNP(1-32) respectively, thereby regulating blood pressure in the heart and promoting natriuresis, diuresis and vasodilation (PubMed:10880574, PubMed:20489134, PubMed:21288900, PubMed:21763278). Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus (PubMed:22437503). Also acts as a regulator of sodium reabsorption in kidney (By similarity). Isoform 2. Has weaker endopeptidase activity compared to isoform 1.
Alternative names
CRN, TMPRSS10, CORIN, Atrial natriuretic peptide-converting enzyme, Corin, Heart-specific serine proteinase ATC2, Pro-ANP-converting enzyme, Transmembrane protease serine 10
Recommended products
Rabbit Recombinant Monoclonal CORIN antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR23361-77
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (ab274320), expandable thumbnail](https://content.abcam.com/products/images/corin-antibody-epr23361-77-ab274320--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17952.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (ab274320)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (ab274320)
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Corin with ab274320 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human cardiac muscle (PMID: 11082206). The section was incubated with ab274320 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Western blot - Anti-Corin antibody [EPR23361-77] (ab274320), expandable thumbnail](https://content.abcam.com/products/images/corin-antibody-epr23361-77-ab274320--western-blot-img579.jpg "Western blot - Anti-Corin antibody [EPR23361-77] (ab274320)")
Western blot - Anti-Corin antibody [EPR23361-77] (ab274320)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Tissue specific protein, highly expressed in heart (PMID: 11082006).
The expression profile observed is consistent with what has been described in the literature (PMID: 29387201, 17996891).
Exposure time: 10 seconds.
All lanes: Western blot - Anti-Corin antibody [EPR23361-77] (ab274320) at 1/1000 dilution
All lanes: Mouse heart tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 116 kDa
Observed band size: 100-180 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (ab274320), expandable thumbnail](https://content.abcam.com/products/images/corin-antibody-epr23361-77-ab274320--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115867.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (ab274320)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (ab274320)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Corin with ab274320 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human kidney. The section was incubated with ab274320 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Western blot - Anti-Corin antibody [EPR23361-77] (ab274320), expandable thumbnail](https://content.abcam.com/products/images/corin-antibody-epr23361-77-ab274320--western-blot-img4925.jpg "Western blot - Anti-Corin antibody [EPR23361-77] (ab274320)")
Western blot - Anti-Corin antibody [EPR23361-77] (ab274320)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Tissue specific protein, highly expressed in heart (PMID: 11082006).
The expression profile observed is consistent with what has been described in the literature (PMID: 29387201, 17996891).
Low expression: human brain (PMID: 11082006).
Exposure time: 3 minutes.
All lanes: Western blot - Anti-Corin antibody [EPR23361-77] (ab274320) at 1/1000 dilution
Lane 1: Human heart tissue lysate at 20 µg
Lane 2: Human brain tissue lysate at 20 µg
SecondaryAll lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 116 kDa
Observed band size: 100-180 kDa
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