Rabbit Polyclonal CORO2A antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CORO2A aa 1-300.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CORO2A aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q92828
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Target data
Alternative names
IR10, WDR2, CORO2A, Coronin-2A, WD repeat-containing protein 2
Recommended products
Rabbit Polyclonal CORO2A antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CORO2A aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CORO2A aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q92828
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CORO2A also known as coronin-2A is a protein within the coronin family involved in actin dynamics. Coronin-2A has a molecular mass of approximately 56 kDa and appears across many tissues especially in the brain heart and skeletal muscle. The protein features WD repeat domains which play roles in protein-protein interactions. These structures help in the assembly of actin filament networks which are important for cell movement and stability.
- Biological function summary
Coronin-2A contributes to actin cytoskeleton reorganization a process essential for cell motility growth and division. Coronin-2A interacts with the Arp2/3 complex an important regulator in the formation of branched actin networks. The protein acts in concert with other actin-binding proteins to modulate actin filament assembly and organization. This modulation is necessary for various cellular activities including signal transduction and intracellular transport.
- Pathways
Coronin-2A functions in the regulation of actin dynamics and the Rac1-dependent cellular processes. The protein plays roles in the Wnt signaling pathway where it aids in modulating cytoskeletal arrangements. Coronin-2A's association with the Rac1 GTPase highlights its importance in pathways involving actin-driven changes directly influencing cellular architecture and communication. This links it to other proteins such as cofilin which also facilitate actin filament remodeling.
- Associated diseases and disorders
Coronin-2A has implications in cancer metastasis and cardiac hypertrophy. In cancer its alteration affects cell motility and metastasis implicating it in the spread of tumor cells. Coronin-2A interacts with proteins like cofilin and the Arp2/3 complex in these processes influencing cellular adhesion and migration. In cardiac hypertrophy changes in coronin-2A expression or function contribute to altered heart muscle cell dynamics and growth potentially affecting heart performance and health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-CORO2A antibody (ab155305)
7.5% SDS PAGE
All lanes: Western blot - Anti-CORO2A antibody (ab155305) at 1/1000 dilution
All lanes: Raji whole cell lysate at 30 µg
Predicted band size: 60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CORO2A antibody (ab155305)
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue, labeling CORO2A with ab155305 at 1/500 dilution.
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