Rabbit Recombinant Monoclonal Coronin 1a/TACO antibody. Suitable for ICC/IF, Flow Cyt (Intra), WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (AB203698), expandable thumbnail](https://content.abcam.com/products/images/coronin-1a-taco-antibody-epr19467-36-ab203698--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img83344.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (AB203698)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (AB203698), expandable thumbnail](https://content.abcam.com/products/images/coronin-1a-taco-antibody-epr19467-36-ab203698--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24024.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (AB203698)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (AB203698), expandable thumbnail](https://content.abcam.com/products/images/coronin-1a-taco-antibody-epr19467-36-ab203698--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25750.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (AB203698)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (AB203698), expandable thumbnail](https://content.abcam.com/products/images/coronin-1a-taco-antibody-epr19467-36-ab203698--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107279.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (AB203698)")
![Western blot - Anti-Coronin 1a/TACO antibody [EPR19467-36] (AB203698), expandable thumbnail](https://content.abcam.com/products/images/coronin-1a-taco-antibody-epr19467-36-ab203698--western-blot-img7414.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Coronin 1a/TACO antibody [EPR19467-36] (AB203698)")
Publications
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- International journal of molecular sciences 24:2023Proteomic Fingerprint of Lung Fibrosis Progression and Response to Therapy in Bleomycin-Induced Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLucrezia Principi,Erica Ferrini,Roberta Ciccimarra,Lisa Pagani,Clizia Chinello,Paolo Previtali,Andrew Smith,Gino Villetti,Matteo Zoboli,Francesca Ravanetti,Franco Fabio Stellari,Fulvio Magni,Isabella PigaPubMed 36901840
- International journal of biological sciences 18:5624-56402022Regulation of Podocyte Injury by CircHIPK3/FUS Complex in Diabetic Kidney Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Liu,Jing Huang,Chunyun Zhang,Yaru Xie,Yiling Cao,Li Tao,Hui Tang,Jihong Lin,Hans-Peter Hammes,Kun Huang,Fan Yi,Hua Su,Chun ZhangPubMed 36263181
- Annals of translational medicine 10:1902022Identification of key biomarkers and immune infiltration in renal interstitial fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhanhong Hu,Yumei Liu,Ye Zhu,Hongxia Cui,Jie PanPubMed 35280428
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| ICC/IF | Flow Cyt (Intra) | WB | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Tested |
| Rat | Expected | Expected | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes.
Alternative names
CORO1, CORO1A, Coronin-1A, Coronin-like protein A, Coronin-like protein p57, Tryptophan aspartate-containing coat protein, Clipin-A, TACO
Recommended products
Rabbit Recombinant Monoclonal Coronin 1a/TACO antibody. Suitable for ICC/IF, Flow Cyt (Intra), WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR19467-36
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698), expandable thumbnail](https://content.abcam.com/products/images/coronin-1a-taco-antibody-epr19467-36-ab203698--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img83344.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Coronin 1a/TACO with ab203698 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on rat spleen (PMID: 17341475) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698), expandable thumbnail](https://content.abcam.com/products/images/coronin-1a-taco-antibody-epr19467-36-ab203698--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24024.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Coronin 1a/TACO with ab203698 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on mouse spleen (PMID: 17341475) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698), expandable thumbnail](https://content.abcam.com/products/images/coronin-1a-taco-antibody-epr19467-36-ab203698--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25750.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Coronin 1a/TACO with ab203698 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human spleen (PMID: 17341475) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698), expandable thumbnail](https://content.abcam.com/products/images/coronin-1a-taco-antibody-epr19467-36-ab203698--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107279.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Coronin 1a/TACO with ab203698 at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human colon (PMID: 17341475) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Western blot - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698), expandable thumbnail](https://content.abcam.com/products/images/coronin-1a-taco-antibody-epr19467-36-ab203698--western-blot-img7414.jpg "Western blot - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)")
Western blot - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)
Blocking and dilution buffer: 5% NFDM/TBST.The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 22223354, PMID: 22364218).
All lanes: Western blot - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698) at 1/1000 dilution
Lane 1: Human brain lysate at 20 µg
Lane 2: Human spleen lysate at 20 µg
Lane 3: Mouse brain lysate at 20 µg
Lane 4: Mouse spleen lysate at 20 µg
Lane 5: Rat spleen lysate at 20 µg
Lane 6: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa, 57 kDa
Exposure time: 26s
![Western blot - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698), expandable thumbnail](https://content.abcam.com/products/images/coronin-1a-taco-antibody-epr19467-36-ab203698--western-blot-img14850.jpg "Western blot - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)")
Western blot - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)
Blocking and dilution buffer: 5% NFDM/TBST.The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22223354, PMID: 22364218).
Negative control: HEK-293.
All lanes: Western blot - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698) at 1/1000 dilution
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 2: Raji (human Burkitt's lymphoma cell line) whole cell lysate at 20 µg
Lane 3: MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 20 µg
Lane 4: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa, 57 kDa
Exposure time: 37s
![Flow Cytometry (Intracellular) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698), expandable thumbnail](https://content.abcam.com/products/images/coronin-1a-taco-antibody-epr19467-36-ab203698--flow-cytometry-intracellular-img67770.jpg "Flow Cytometry (Intracellular) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)")
Flow Cytometry (Intracellular) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)
Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293 (human epithelial cell line from embryonic kidney) (Left panel) and Jurkat (human T cell leukemia cell line from peripheral blood) (Right panel) cells labeling Coronin 1a/TACO with ab203698 at 1/500 dilution (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluorr® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
Negative control: HEK-293 (PMID:17341475).
![Immunocytochemistry/ Immunofluorescence - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698), expandable thumbnail](https://content.abcam.com/products/images/coronin-1a-taco-antibody-epr19467-36-ab203698--immunocytochemistry-immunofluorescence-img41284.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)")
Immunocytochemistry/ Immunofluorescence - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling Coronin 1a/TACO with ab203698 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in Jurkat cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution.
Control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
Negative control: HEK-293（PMID:17341475).
![Western blot - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698), expandable thumbnail](https://content.abcam.com/products/images/coronin-1a-taco-antibody-epr19467-36-ab203698--western-blot-img14850.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)")
![Flow Cytometry (Intracellular) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698), expandable thumbnail](https://content.abcam.com/products/images/coronin-1a-taco-antibody-epr19467-36-ab203698--flow-cytometry-intracellular-img67770.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)")
![Immunocytochemistry/ Immunofluorescence - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698), expandable thumbnail](https://content.abcam.com/products/images/coronin-1a-taco-antibody-epr19467-36-ab203698--immunocytochemistry-immunofluorescence-img41284.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Coronin 1a/TACO antibody [EPR19467-36] (ab203698)")
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