Anti-Coronin 1a/TACO antibody [EPR19467-81]

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Coronin 1a/TACO antibody [EPR19467-81] (AB243090)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293 (human epithelial cell line from embryonic kidney) (Left panel) and Jurkat (human epithelial cell line from embryonic kidney) (Right panel) cells labeling Coronin 1a/TACOwith ab243090 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

Negative control : HEK-293 (PMID : 17341475).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-81] (AB243090)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Coronin 1a/TACO with ab243090 at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human colon (PMID : 17341475) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Coronin 1a/TACO antibody [EPR19467-81] (AB243090)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling Coronin 1a/TACO with ab243090 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in Jurkat cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution.

Control : Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Negative control : HEK-293 (PMID : 17341475).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-81] (AB243090)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Coronin 1a/TACO with ab243090 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human spleen (PMID : 17341475) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• WB

Unknown

Western blot - Anti-Coronin 1a/TACO antibody [EPR19467-81] (AB243090)

Blocking and dilution buffer : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 22223354, PMID : 22364218).

All lanes:

Western blot - Anti-Coronin 1a/TACO antibody [EPR19467-81] (ab243090) at 1/1000 dilution

Lane 1:

Human brain lysate at 20 µg

Lane 2:

Human spleen lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/100000 dilution

Predicted band size: 51 kDa

Observed band size: 51 kDa,57 kDa

false

Exposure time: 3min

• WB

Unknown

Western blot - Anti-Coronin 1a/TACO antibody [EPR19467-81] (AB243090)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times :

Lane 1 : 3 seconds.

Lanes 2-4 : 10 seconds.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 22223354, PMID : 22364218).

Negative control : HEK-293.

All lanes:

Western blot - Anti-Coronin 1a/TACO antibody [EPR19467-81] (ab243090) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 3:

MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 20 µg

Lane 4:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 51 kDa

Observed band size: 51 kDa,57 kDa

false