Anti-Coronin-1C antibody [EPR25365-24] (ab283693)

Rabbit Recombinant Monoclonal Coronin-1C antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples.

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Images

Western blot - Anti-Coronin-1C antibody [EPR25365-24] (AB283693), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Not recommended	Not recommended	Not recommended
Mouse	Expected	Tested	Not recommended	Not recommended	Not recommended
Rat	Expected	Tested	Not recommended	Not recommended	Not recommended
Recombinant full length protein - Human	Not recommended	Tested	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes IP suitable for human only. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes -
Species Recombinant full length protein - Human	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat, Recombinant full length protein - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat, Recombinant full length protein - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes IHC suitable for human only. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes IHC suitable for human only. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes IHC suitable for human only. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Recombinant full length protein - Human	Dilution info -	Notes -

Target data

See full target information CORO1C

Function

Plays a role in directed cell migration by regulating the activation and subcellular location of RAC1 (PubMed:25074804, PubMed:25925950). Increases the presence of activated RAC1 at the leading edge of migrating cells (PubMed:25074804, PubMed:25925950). Required for normal organization of the cytoskeleton, including the actin cytoskeleton, microtubules and the vimentin intermediate filaments (By similarity). Plays a role in endoplasmic reticulum-associated endosome fission: localizes to endosome membrane tubules and promotes recruitment of TMCC1, leading to recruitment of the endoplasmic reticulum to endosome tubules for fission (PubMed:30220460). Endosome membrane fission of early and late endosomes is essential to separate regions destined for lysosomal degradation from carriers to be recycled to the plasma membrane (PubMed:30220460). Required for normal cell proliferation, cell migration, and normal formation of lamellipodia (By similarity). Required for normal distribution of mitochondria within cells (By similarity). Isoform 3. Involved in myogenic differentiation.

Alternative names

CRN2, CRNN4, CORO1C, Coronin-1C, Coronin-3, hCRNN4

Recommended products

Rabbit Recombinant Monoclonal Coronin-1C antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR25365-24
Purification technique: Affinity purification Protein A
Specificity: IHC and IP suitable for human only.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

Western blot - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes
All lanes: Western blot - Anti-Coronin-1C antibody [EPR25365-24] (ab283693) at 1/1000 dilution
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 3: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg
Lane 4: HL-1 (mouse atrial muscle cell), whole cell lysate at 20 µg
Lane 5: C6 (rat glial tumor glial cell), whole cell lysate at 20 µg
Lane 6: Human heart tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
Western blot - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)
Blocking and diluting buffer concentration: 5% NFDM/TBST
Exposure time 26 seconds
All lanes: Western blot - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: Hela transfected with CORO1C siRNA 1 whole cell lysate at 20 µg
Lane 3: Hela transfected with CORO1C siRNA 2 whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
Western blot - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody has no cross-reaction with human Coronin-1B, Coronin-6 and Coronin-1A.
All the recombinant proteins were made in-house.
All the samples are E.coli extracts containing Coronin-1C, Coronin-1B, Coronin-6 or Coronin-1A recombinant protein respectively.
Exposure time: 26 seconds
All lanes: Western blot - Anti-Coronin-1C antibody [EPR25365-24] (ab283693) at 1/1000 dilution
Lane 1: His-tagged human Coronin-1C recombinant protein at 0.005 µg
Lane 2: His-tagged human Coronin-1B recombinant protein at 0.005 µg
Lane 3: His-tagged human Coronin-6 recombinant protein at 0.005 µg
Lane 4: His-tagged human Coronin-1A recombinant protein at 0.005 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)
Immunohistochemical analysis of paraffin-embedded Human breast tissue labelling Coronin-1C with ab283693 at 1/2000 (0.301 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human breast (PMID: 30065298). The section was incubated with ab283693 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunoprecipitation - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)
Coronin-1C was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 ug with ab283693 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283693 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 ug
Lane 2: ab283693 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab283693 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
All lanes: Immunoprecipitation - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)
Predicted band size: 53 kDa
Observed band size: 53 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labelling Coronin-1C with ab283693 at 1/2000 (0.301 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human breast cancer (PMID: 30065298). The section was incubated with ab283693 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)
Immunohistochemical analysis of paraffin-embedded Human lymphoid nodule tissue labelling Coronin-1C with ab283693 at 1/2000 (0.301 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human lymphoid nodule (PMID: 20690162). The section was incubated with ab283693 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Western blot - Anti-Coronin-1C antibody [EPR25365-24] (ab283693)
Blocking buffer and concentration: ½ volume of Odyssey Blocking Buffer (TBS)+ ½ volume of 0.1% TBS Diluting buffer and concentration: ½ volume of Odyssey Blocking Buffer (TBS)+ ½ volume of 0.1% TBST

False colour image of Western blot: Anti-Coronin-1C antibody [EPR25365-24] (ab283693) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.

In Western blot, ab283693 was shown to bind specifically to Coronin-1C. A band was observed at 53 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in Coronin-1C knockout cell line Human CORO1C (Coronin-1C) knockout HEK-293T cell line ab266381 (knockout cell lysate (Human CORO1C (Coronin-1C) knockout HEK-293T cell lysate ab258377).

To generate this image, wild-type and Coronin-1C knockout HEK-293T cell lysates were analyzed. First, samples were run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.
Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-Coronin-1C antibody [EPR25365-24] (ab283693) at 1/1000 dilution
Lane 1: Wild-type 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: Western blot - Human CORO1C (Coronin-1C) knockout HEK-293T cell lysate (Human CORO1C (Coronin-1C) knockout HEK-293T cell lysate ab258377) at 20 µg
Lane 2: Western blot - Human CORO1C (Coronin-1C) knockout HEK-293T cell line (Human CORO1C (Coronin-1C) knockout HEK-293T cell line ab266381)
Lane 2: Western blot - Human CORO1C (Coronin-1C) knockout HEK-293T cell lysate (Human CORO1C (Coronin-1C) knockout HEK-293T cell lysate ab258377)
Secondary
Lanes 1 - 2: Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution
Lanes 1 - 2: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa