AB231620

Anti-Cortactin antibody

Name: Anti-Cortactin antibody (ab231620) | Abcam
Brand: Abcam Limited
SKU: AB231620
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Cortactin antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Cttn aa 1 to C-terminus.

View Alternative Names

Ems1, Cttn, Src substrate cortactin

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cortactin antibody (AB231620)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cortactin antibody (AB231620)

Formalin-fixed, paraffin-embedded mouse liver tissue stained for Cortactin with ab231620 at 30 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cortactin antibody (AB231620)

Formalin-fixed, paraffin-embedded mouse uterus tissue stained for Cortactin with ab231620 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Cortactin antibody (AB231620)

All lanes:

Western blot - Anti-Cortactin antibody (ab231620) at 2 µg/mL

All lanes:

Recombinant mouse Cortactin protein

Predicted band size: 62 kDa

false

Supplier Data

Western blot - Anti-Cortactin antibody (AB231620)

All lanes:

Western blot - Anti-Cortactin antibody (ab231620) at 1 µg/mL

Lane 1:

Mouse testis tissue lysate

Lane 2:

Mouse brain tissue lysate

Lane 3:

Mouse pancreas tissue lysate

Lane 4:

Mouse parotid gland tissue lysate

Secondary

All lanes:

HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 62 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Mouse Cttn aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q60598

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Contributes to the organization of the actin cytoskeleton and cell shape (PubMed : 17403031). Plays a role in the formation of lamellipodia and in cell migration (By similarity). Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (PubMed : 22262902). Plays a role in focal adhesion assembly and turnover (By similarity). In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (By similarity). Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed : 17959782). Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity). Required for stabilization of KCNH1 channels at the cell membrane (By similarity).

See full target information Cttn

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 14: PubMed35884490

2022

Ras-Related Protein Rab-32 and Thrombospondin 1 Confer Resistance to the EGFR Tyrosine Kinase Inhibitor Osimertinib by Activating Focal Adhesion Kinase in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zeinab Kosibaty,Odd Terje Brustugun,Inger Johanne Zwicky Eide,Georgios Tsakonas,Oscar Grundberg,Luigi De Petris,Marc McGowan,Per Hydbring,Simon Ekman

View all publications

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Product promise

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