Rabbit Polyclonal Cortactin antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Cttn aa 1 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Mouse Cttn aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q60598
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Predicted | Predicted |
| Mouse | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.5-2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5-20 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Contributes to the organization of the actin cytoskeleton and cell shape (PubMed:17403031). Plays a role in the formation of lamellipodia and in cell migration (By similarity). Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (PubMed:22262902). Plays a role in focal adhesion assembly and turnover (By similarity). In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (By similarity). Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed:17959782). Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity). Required for stabilization of KCNH1 channels at the cell membrane (By similarity).
Alternative names
Ems1, Cttn, Src substrate cortactin
Recommended products
Rabbit Polyclonal Cortactin antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Cttn aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Cttn aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q60598
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cortactin antibody (ab231620)
Formalin-fixed, paraffin-embedded mouse uterus tissue stained for Cortactin with ab231620 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-Cortactin antibody (ab231620)
All lanes: Western blot - Anti-Cortactin antibody (ab231620) at 1 µg/mL
Lane 1: Mouse testis tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Mouse pancreas tissue lysate
Lane 4: Mouse parotid gland tissue lysate
SecondaryAll lanes: HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 62 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cortactin antibody (ab231620)
Formalin-fixed, paraffin-embedded mouse liver tissue stained for Cortactin with ab231620 at 30 μg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-Cortactin antibody (ab231620)
All lanes: Western blot - Anti-Cortactin antibody (ab231620) at 2 µg/mL
All lanes: Recombinant mouse Cortactin protein
Predicted band size: 62 kDa
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