Anti-Cortactin antibody [EP1922Y] - BSA and Azide free

5 product images

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  Immunocytochemistry/ Immunofluorescence - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)

  Anti-Cortactin antibody [EP1922Y] ab81208 staining Cortactin in wild-type HAP1 cells (top panel) and Cortactin knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Anti-Cortactin antibody [EP1922Y] ab81208 at 1/1000 dilution and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cortactin antibody [EP1922Y] ab81208).

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  Immunoprecipitation - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)

  Purified Anti-Cortactin antibody [EP1922Y] ab81208 at 1/50 dilution (2μg) immunoprecipitating Cortactin in HeLa whole cell lysate.
  Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
  Lane 2 (+): Anti-Cortactin antibody [EP1922Y] ab81208 + HeLa whole cell lysate.
  Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Cortactin antibody [EP1922Y] ab81208 in HeLa whole cell lysate.
  VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
  Blocking Buffer and concentration: 5% NFDM/TBST.
  Diluting buffer and concentration: 5% NFDM/TBST.
  Observed band size: 62 kDa
  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cortactin antibody [EP1922Y] ab81208).

  All lanes: Immunoprecipitation - Anti-Cortactin antibody [EP1922Y] (Anti-Cortactin antibody [EP1922Y] ab81208)

  Predicted band size: 62 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)

  Immunohistochemical staining of paraffin-embedded human breast carcinoma using 1/100 Anti-Cortactin antibody [EP1922Y] ab81208.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cortactin antibody [EP1922Y] ab81208).

  Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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  Immunocytochemistry/ Immunofluorescence - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)

  Immunofluorescent staining of MCF7 cells using 1/100 ab81208This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cortactin antibody [EP1922Y] ab81208).

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  Flow Cytometry (Intracellular) - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)

  Overlay histogram showing HeLa cells stained with Anti-Cortactin antibody [EP1922Y] ab81208 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-Cortactin antibody [EP1922Y] ab81208, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Cortactin antibody [EP1922Y] ab81208).