Rabbit Recombinant Monoclonal Cortactin antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (AB247383), expandable thumbnail](https://content.abcam.com/products/images/cortactin-antibody-epr1923y-bsa-and-azide-free-ab247383--western-blot-img3237.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (AB247383)")
![Western blot - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (AB247383), expandable thumbnail](https://content.abcam.com/products/images/cortactin-antibody-epr1923y-bsa-and-azide-free-ab247383--western-blot-img40715.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (AB247383)")
![Flow Cytometry (Intracellular) - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (AB247383), expandable thumbnail](https://content.abcam.com/products/images/cortactin-antibody-epr1923y-bsa-and-azide-free-ab247383--flow-cytometry-intracellular-img56621.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (AB247383)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Tested | Not recommended | Expected |
| Rat | Not recommended | Tested | Not recommended | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Contributes to the organization of the actin cytoskeleton and cell shape (PubMed:21296879). Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (By similarity). Plays a role in focal adhesion assembly and turnover (By similarity). In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (PubMed:20861316). Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed:17959782). Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity). Required for stabilization of KCNH1 channels at the cell membrane (PubMed:23144454). Plays a role in the invasiveness of cancer cells, and the formation of metastases (PubMed:16636290).
Alternative names
EMS1, CTTN, Src substrate cortactin, Amplaxin, Oncogene EMS1
Recommended products
Rabbit Recombinant Monoclonal Cortactin antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR1923Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247383 is the carrier-free version of Anti-Cortactin antibody [EPR1923Y] ab68438.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383), expandable thumbnail](https://content.abcam.com/products/images/cortactin-antibody-epr1923y-bsa-and-azide-free-ab247383--western-blot-img3237.jpg "Western blot - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383)")
Western blot - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383)
This data was developed using Anti-Cortactin antibody [EPR1923Y] ab68438, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (40 μg)
Lane 2: Cortactin knockout HAP1 cell lysate (40 μg)
Lane 3: NIH3T3 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Cortactin antibody [EPR1923Y] ab68438 observed at 70 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-Cortactin antibody [EPR1923Y] ab68438 was shown to specifically recognize Cortactin in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when Cortactin knockout samples were examined. Wild-type and Cortactin knockout samples were subjected to SDS-PAGE. Anti-Cortactin antibody [EPR1923Y] ab68438 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Cortactin antibody [EPR1923Y] (Anti-Cortactin antibody [EPR1923Y] ab68438)
Predicted band size: 62 kDa
![Western blot - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383), expandable thumbnail](https://content.abcam.com/products/images/cortactin-antibody-epr1923y-bsa-and-azide-free-ab247383--western-blot-img40715.jpg "Western blot - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383)")
Western blot - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383)
This data was developed using Anti-Cortactin antibody [EPR1923Y] ab68438, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383) at 1/5000 dilution
Lane 1: C6 cell lysate at 10 µg
Lane 2: MCF-7 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: A431 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 62 kDa
Observed band size: 80 kDa
![Flow Cytometry (Intracellular) - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383), expandable thumbnail](https://content.abcam.com/products/images/cortactin-antibody-epr1923y-bsa-and-azide-free-ab247383--flow-cytometry-intracellular-img56621.jpg "Flow Cytometry (Intracellular) - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383)")
Flow Cytometry (Intracellular) - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383)
This data was developed using Anti-Cortactin antibody [EPR1923Y] ab68438, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized MCF-7 cells using Anti-Cortactin antibody [EPR1923Y] ab68438 1/30 dilution (pink) or a rabbit IgG (negative) (green).
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