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AB47768

Anti-Cortactin (phospho Y421) antibody

4

(2 Reviews)

|

(9 Publications)

Rabbit Polyclonal Cortactin phospho Y421 antibody. Suitable for ELISA, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human CTTN pY421 aa 350-450.

View Alternative Names

EMS1, CTTN, Src substrate cortactin, Amplaxin, Oncogene EMS1

1 Images
Western blot - Anti-Cortactin (phospho Y421) antibody (AB47768)
  • WB

Unknown

Western blot - Anti-Cortactin (phospho Y421) antibody (AB47768)

Typically 5-30ug of total protein was loaded per lane of the gel. The cells were treated with 150 uM H2O2.

All lanes:

Western blot - Anti-Cortactin (phospho Y421) antibody (ab47768) at 1/500 dilution

Lane 1:

Hela cell extract, treated with H<sub>2</sub>O<sub>2</sub>

Lane 2:

Hela cell extract, untreated

Predicted band size: 62 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ELISA, WB

applications

Immunogen

Synthetic Peptide within Human CTTN pY421 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

Q14247

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Contributes to the organization of the actin cytoskeleton and cell shape (PubMed : 21296879). Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (By similarity). Plays a role in focal adhesion assembly and turnover (By similarity). In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (PubMed : 20861316). Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed : 17959782). Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity). Required for stabilization of KCNH1 channels at the cell membrane (PubMed : 23144454). Plays a role in the invasiveness of cancer cells, and the formation of metastases (PubMed : 16636290).
See full target information CTTN pY421
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 55:779-793 PubMed37009792

2023

SAMHD1-induced endosomal FAK signaling promotes human renal clear cell carcinoma metastasis by activating Rac1-mediated lamellipodia protrusion.

Applications

Unspecified application

Species

Unspecified reactive species

Sunho An,Tam Thuy Lu Vo,Taekwon Son,Hoon Choi,Jinyoung Kim,Juyeon Lee,Byung Hoon Kim,Misun Choe,Eunyoung Ha,Young-Joon Surh,Kyu-Won Kim,Ji Hae Seo

American journal of physiology. Lung cellular and molecular physiology 322:L890-L897 PubMed35503995

2022

Cortactin loss protects against hemin-induced acute lung injury in sickle cell disease.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole M Jones,Justin R Sysol,Sunit Singla,Patricia Smith,George E Sandusky,Huashan Wang,Viswanathan Natarajan,Steven M Dudek,Roberto F Machado

Experimental and therapeutic medicine 22:952 PubMed34335894

2021

Autophagy promotes invadopodia formation in human ovarian cancer cells via the p62-extracellular signal-regulated kinase 1/2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zizhen Zhou,Jia Zhao,Yanan Liu,Xiaoyu Yan,Hongyu Sun,Meihui Xia,Jing Su

Cell reports 34:108800 PubMed33657382

2021

Endothelin-1 drives invadopodia and interaction with mesothelial cells through ILK.

Applications

Unspecified application

Species

Unspecified reactive species

Ilenia Masi,Valentina Caprara,Francesca Spadaro,Lidia Chellini,Rosanna Sestito,Andrea Zancla,Alberto Rainer,Anna Bagnato,Laura Rosanò

eLife 9: PubMed32844749

2020

Arginine methylation of SHANK2 by PRMT7 promotes human breast cancer metastasis through activating endosomal FAK signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Yingqi Liu,Lingling Li,Xiaoqing Liu,Yibo Wang,Lingxia Liu,Lu Peng,Jiayuan Liu,Lian Zhang,Guannan Wang,Hongyuan Li,Dong-Xu Liu,Baiqu Huang,Jun Lu,Yu Zhang

Cell chemical biology 26:1081-1094.e6 PubMed31130521

2019

Time-Variant SRC Kinase Activation Determines Endothelial Permeability Response.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer E Klomp,Mark Shaaya,Jacob Matsche,Rima Rebiai,Jesse S Aaron,Kerrie B Collins,Vincent Huyot,Annette M Gonzalez,William A Muller,Teng-Leong Chew,Asrar B Malik,Andrei V Karginov

Matrix biology : journal of the International Society for Matrix Biology 81:17-33 PubMed30367951

2018

Regulation of extracellular matrix degradation and metastatic spread by IQGAP1 through endothelin-1 receptor signalling in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lidia Chellini,Valentina Caprara,Francesca Spadaro,Rosanna Sestito,Anna Bagnato,Laura Rosanò

PloS one 7:e33662 PubMed22479425

2012

Cortactin tyrosine phosphorylation promotes its deacetylation and inhibits cell spreading.

Applications

WB

Species

Unspecified reactive species

Eugenia Meiler,Elvira Nieto-Pelegrín,Narcisa Martinez-Quiles

The Journal of biological chemistry 284:25302-13 PubMed19589785

2009

HER2 carboxyl-terminal fragments regulate cell migration and cortactin phosphorylation.

Applications

WB

Species

Human

Jesús García-Castillo,Kim Pedersen,Pier-Davide Angelini,Joan Josep Bech-Serra,Núria Colomé,Matthew Paul Cunningham,Josep Lluis Parra-Palau,Francesc Canals,José Baselga,Joaquín Arribas
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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