Rabbit Recombinant Monoclonal Cortisol antibody. Suitable for WB, I-ELISA and reacts with Chemical samples.
Images
![Western blot - Anti-Cortisol antibody [EPR24434-27] (AB316183), expandable thumbnail](https://content.abcam.com/products/images/cortisol-antibody-epr24434-27-ab316183--western-blot-img426389.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cortisol antibody [EPR24434-27] (AB316183)")
![Indirect ELISA - Anti-Cortisol antibody [EPR24434-27] (AB316183), expandable thumbnail](https://content.abcam.com/products/images/cortisol-antibody-epr24434-27-ab316183--indirect-elisa-img426484.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Cortisol antibody [EPR24434-27] (AB316183)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | I-ELISA | IHC-P | IP | IHC-Fr | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|---|
| Chemical | Tested | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 1/1000 | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 1000 ng/mL | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes - |
Target data
Recommended products
Rabbit Recombinant Monoclonal Cortisol antibody. Suitable for WB, I-ELISA and reacts with Chemical samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR24434-27
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Cortisol antibody [EPR24434-27] (ab316183), expandable thumbnail](https://content.abcam.com/products/images/cortisol-antibody-epr24434-27-ab316183--western-blot-img426389.jpg "Western blot - Anti-Cortisol antibody [EPR24434-27] (ab316183)")
Western blot - Anti-Cortisol antibody [EPR24434-27] (ab316183)
All lanes: Western blot - Anti-Cortisol antibody [EPR24434-27] (ab316183) at 1/1000 dilution
Lane 1: Cortisol-crosslink-BSA at 10 ng with 5% NFDM/TBST
Lane 2: Hydrocortisone 21-hemisuccinate-crosslink-BSA at 10 ng with 5% NFDM/TBST
Lane 3: Gentamicin-crosslink-BSA at 10 ng with 5% NFDM/TBST
Lane 4: BSA at 10 ng with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 15s
![Indirect ELISA - Anti-Cortisol antibody [EPR24434-27] (ab316183), expandable thumbnail](https://content.abcam.com/products/images/cortisol-antibody-epr24434-27-ab316183--indirect-elisa-img426484.jpg "Indirect ELISA - Anti-Cortisol antibody [EPR24434-27] (ab316183)")
Indirect ELISA - Anti-Cortisol antibody [EPR24434-27] (ab316183)
Indirect ELISA analysis of ab316183 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Cortisol-crosslink-BSA, Gentamicin-crosslink-BSA.
Antigen concentration: 1000 ng/ml
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com