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AB316184

Anti-Cortisol antibody [EPR24434-27] - BSA and Azide free

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Rabbit Recombinant Monoclonal Cortisol antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Chemical samples.
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Indirect ELISA - Anti-Cortisol antibody [EPR24434-27] - BSA and Azide free (AB316184)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Cortisol antibody [EPR24434-27] - BSA and Azide free (AB316184)

This data was developed using ab316183, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab316183 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Cortisol-crosslink-BSA, Gentamicin-crosslink-BSA.

Antigen concentration : 1000 ng/ml

Western blot - Anti-Cortisol antibody [EPR24434-27] - BSA and Azide free (AB316184)
  • WB

Supplier Data

Western blot - Anti-Cortisol antibody [EPR24434-27] - BSA and Azide free (AB316184)

This data was developed using ab316183, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cortisol antibody [EPR24434-27] (<a href='/en-us/products/primary-antibodies/cortisol-antibody-epr24434-27-ab316183'>ab316183</a>) at 1/1000 dilution

Lane 1:

Cortisol-crosslink-BSA at 10 ng

Lane 2:

Hydrocortisone 21-hemisuccinate-crosslink-BSA at 10 ng

Lane 3:

Gentamicin-crosslink-BSA at 10 ng

Lane 4:

BSA at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 15s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24434-27

Isotype

IgG

Carrier free

Yes

Applications

I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab316184 is the carrirer-free version of ab316183.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Cortisol
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Cortisol ELISA Kit

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com