Anti-COUP TF1 antibody [EPR10841]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-COUP TF1 antibody [EPR10841] (AB181137)

Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Coup TF1 with Purified ab181137 at 1 : 100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1 : 200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] (AB181137)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling COUP TF1 with Purified ab181137 at 1 : 100 dilution. Heat mediated antigen retrieval was performed using citrate buffer, pH6.0 . Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] (AB181137)

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma staining COUP TF1 using unpurified ab181137 at 1/100. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody, and Hematoxylin for counterstaining.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-COUP TF1 antibody [EPR10841] (AB181137)

Intracellular flow cytometric analysis of COUP TF1 labeling 2% paraformaldehyde fixed 293 cells using unpurified ab181137 at 1/70 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) secondary antibody was used at 1/150 dilution.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-COUP TF1 antibody [EPR10841] (AB181137)

Intracellular Flow Cytometry analysis of MCF7 (Human cervix adenocarcinoma epithelial cell) cells labeling COUP TF1 with purified ab181137 at 1/500 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] (AB181137)

Immunohistochemical analysis of paraffin-embedded Human glioblastoma staining COUP TF1 using unpurified ab181137 at 1/100. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody, and Hematoxylin for counterstaining.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-COUP TF1 antibody [EPR10841] (AB181137)

Immunocytochemistry/Immunofluorescence analysis of MCF-7cells labelling COUP TF1 with unpurified ab181137 at 1/500. Cells were fixed with 4% Paraformaldehyde. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

Control : PBS only.
Nuclear counter stain : DAPI.

• WB

Supplier Data

Western blot - Anti-COUP TF1 antibody [EPR10841] (AB181137)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-COUP TF1 antibody [EPR10841] (ab181137) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

Human fetal kidney lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

false

• WB

Lab

Western blot - Anti-COUP TF1 antibody [EPR10841] (AB181137)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-COUP TF1 antibody [EPR10841] (ab181137) at 1/5000 dilution

Lane 1:

Human fetal brain lysates at 20 µg

Lane 2:

Human fetal kidney lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 46 kDa

false

• WB

Lab

Western blot - Anti-COUP TF1 antibody [EPR10841] (AB181137)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-COUP TF1 antibody [EPR10841] (ab181137) at 1/1000 dilution

All lanes:

Rat brain lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 46 kDa

false

• WB

Lab

Western blot - Anti-COUP TF1 antibody [EPR10841] (AB181137)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-COUP TF1 antibody [EPR10841] (ab181137) at 1/5000 dilution

All lanes:

Mouse E12.5 brain lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 46 kDa

false