Anti-COUP TF1 antibody [EPR10841] Rabbit monoclonal (ab181137)

Rabbit Recombinant Monoclonal COUP TF1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 15 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] (AB181137), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Expected
Rat	Expected	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/250	Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/250	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/70.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information NR2F1

Function

Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.

Alternative names

EAR3, ERBAL3, TFCOUP1, NR2F1, COUP transcription factor 1, COUP-TF1, COUP transcription factor I, Nuclear receptor subfamily 2 group F member 1, V-erbA-related protein 3, COUP-TF I, EAR-3

Recommended products

Rabbit Recombinant Monoclonal COUP TF1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 15 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR10841
Purification technique: Affinity purification Protein A
Specificity: The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

COUP TF1 also known as COUP-TFI COUP-TF1 COUPF protein COUP 70 and counter COUP functions as a transcription factor. It belongs to the nuclear receptor family of proteins and has a molecular mass of approximately 47 kDa. This protein expresses in a variety of tissues including the central nervous system heart and developing organs. It plays a role in regulating gene expression through binding to DNA sequences ultimately controlling developmental processes and cellular differentiation.

Biological function summary

COUP TF1 influences numerous systems by regulating genes involved in neuronal development and organogenesis. This protein is a core component of transcription complexes interacting with other nuclear receptors and co-regulators to modulate its effects. The activity of COUP TF1 also extends to the regulation of neuronal subtype specification and synaptic connectivity signifying its importance in the central nervous system development.

Pathways

COUP TF1 participates in essential signaling cascades such as the Notch and retinoic acid pathways. It modulates the retinoic acid response by interacting with the retinoic acid receptor (RAR) thereby influencing cell differentiation and growth. Additionally COUP TF1 interacts with the Notch signaling pathway affecting neural lineage decisions and maintenance. Its link to other transcription factors and receptors highlights its integrative role in various cellular contexts.

Associated diseases and disorders

COUP TF1 alterations associate with cognitive and developmental disorders. Its dysregulation is implicated in conditions like mental retardation and schizophrenia. The protein's connection to disorders often involves its interaction with other proteins and signaling pathways such as those linked to neural development. COUP TF1's interplay with the retinoic acid receptor influences neurological traits and misregulation can contribute to disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] (ab181137)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling COUP TF1 with Purified ab181137 at 1:100 dilution. Heat mediated antigen retrieval was performed using citrate buffer, pH6.0 . Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunocytochemistry/ Immunofluorescence - Anti-COUP TF1 antibody [EPR10841] (ab181137)
Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Coup TF1 with Purified ab181137 at 1:100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry (Intracellular) - Anti-COUP TF1 antibody [EPR10841] (ab181137)
Intracellular Flow Cytometry analysis of MCF7 (Human cervix adenocarcinoma epithelial cell) cells labeling COUP TF1 with purified ab181137 at 1/500 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-COUP TF1 antibody [EPR10841] (ab181137)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-COUP TF1 antibody [EPR10841] (ab181137) at 1/5000 dilution
Lane 1: Human fetal brain lysates at 20 µg
Lane 2: Human fetal kidney lysates at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 46 kDa
Western blot - Anti-COUP TF1 antibody [EPR10841] (ab181137)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-COUP TF1 antibody [EPR10841] (ab181137) at 1/1000 dilution
All lanes: Rat brain lysates at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 46 kDa
Western blot - Anti-COUP TF1 antibody [EPR10841] (ab181137)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-COUP TF1 antibody [EPR10841] (ab181137) at 1/5000 dilution
All lanes: Mouse E12.5 brain lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 46 kDa
Immunocytochemistry/ Immunofluorescence - Anti-COUP TF1 antibody [EPR10841] (ab181137)
Immunocytochemistry/Immunofluorescence analysis of MCF-7cells labelling COUP TF1 with unpurified ab181137 at 1/500. Cells were fixed with 4% Paraformaldehyde. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

Control: PBS only.
Nuclear counter stain: DAPI.
Western blot - Anti-COUP TF1 antibody [EPR10841] (ab181137)
COUP TF1 Western blot staining using rabbit Anti-COUP TF1 antibody
All lanes: Western blot - Anti-COUP TF1 antibody [EPR10841] (ab181137) at 1/1000 dilution
Lane 1: Human fetal brain lysate at 20 µg
Lane 2: Human fetal kidney lysate at 20 µg
Lane 3: 293 cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] (ab181137)
Immunohistochemical analysis of paraffin-embedded Human glioblastoma staining COUP TF1 using unpurified ab181137 at 1/100. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody, and Hematoxylin for counterstaining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] (ab181137)
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma staining COUP TF1 using unpurified ab181137 at 1/100. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody, and Hematoxylin for counterstaining.
Flow Cytometry (Intracellular) - Anti-COUP TF1 antibody [EPR10841] (ab181137)
Intracellular flow cytometric analysis of COUP TF1 labeling 2% paraformaldehyde fixed 293 cells using unpurified ab181137 at 1/70 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) secondary antibody was used at 1/150 dilution.