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Rabbit Recombinant Monoclonal COUP TF1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (AB224272), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (AB224272), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (AB224272), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (AB224272), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (AB224272), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Tested
Tested
Mouse
Predicted
Expected
Predicted
Predicted
Rat
Predicted
Expected
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

7 products for Alternative Version

Target data

Function

Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.

Alternative names

EAR3, ERBAL3, TFCOUP1, NR2F1, COUP transcription factor 1, COUP-TF1, COUP transcription factor I, Nuclear receptor subfamily 2 group F member 1, V-erbA-related protein 3, COUP-TF I, EAR-3

Recommended products

Rabbit Recombinant Monoclonal COUP TF1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR10841
Purification technique
Affinity purification Protein A
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab224272 is the carrier-free version of Anti-COUP TF1 antibody [EPR10841] ab181137.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

COUP TF1 also known as COUP-TFI COUP-TF1 COUPF protein COUP 70 and counter COUP functions as a transcription factor. It belongs to the nuclear receptor family of proteins and has a molecular mass of approximately 47 kDa. This protein expresses in a variety of tissues including the central nervous system heart and developing organs. It plays a role in regulating gene expression through binding to DNA sequences ultimately controlling developmental processes and cellular differentiation.

Biological function summary

COUP TF1 influences numerous systems by regulating genes involved in neuronal development and organogenesis. This protein is a core component of transcription complexes interacting with other nuclear receptors and co-regulators to modulate its effects. The activity of COUP TF1 also extends to the regulation of neuronal subtype specification and synaptic connectivity signifying its importance in the central nervous system development.

Pathways

COUP TF1 participates in essential signaling cascades such as the Notch and retinoic acid pathways. It modulates the retinoic acid response by interacting with the retinoic acid receptor (RAR) thereby influencing cell differentiation and growth. Additionally COUP TF1 interacts with the Notch signaling pathway affecting neural lineage decisions and maintenance. Its link to other transcription factors and receptors highlights its integrative role in various cellular contexts.

Associated diseases and disorders

COUP TF1 alterations associate with cognitive and developmental disorders. Its dysregulation is implicated in conditions like mental retardation and schizophrenia. The protein's connection to disorders often involves its interaction with other proteins and signaling pathways such as those linked to neural development. COUP TF1's interplay with the retinoic acid receptor influences neurological traits and misregulation can contribute to disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling COUP TF1 with Purified Anti-COUP TF1 antibody [EPR10841] ab181137 at 1:100 dilution. Heat mediated antigen retrieval was performed using citrate buffer, pH6.0 . Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-COUP TF1 antibody [EPR10841] ab181137).

  • Immunocytochemistry/ Immunofluorescence - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)

    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Coup TF2 with Purified Anti-COUP TF1 antibody [EPR10841] ab181137 at 1:100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-COUP TF1 antibody [EPR10841] ab181137).

  • Flow Cytometry (Intracellular) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)

    Intracellular Flow Cytometry analysis of MCF7 (Human cervix adenocarcinoma epithelial cell) cells labeling COUP TF1 with purified Anti-COUP TF1 antibody [EPR10841] ab181137 at 1/500 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-COUP TF1 antibody [EPR10841] ab181137).

  • Immunocytochemistry/ Immunofluorescence - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)

    Immunocytochemistry/Immunofluorescence analysis of MCF-7cells labelling COUP TF1 with unpurified Anti-COUP TF1 antibody [EPR10841] ab181137 at 1/500. Cells were fixed with 4% Paraformaldehyde. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

    Control: PBS only.
    Nuclear counter stain: DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-COUP TF1 antibody [EPR10841] ab181137).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)

    Immunohistochemical analysis of paraffin-embedded Human glioblastoma staining COUP TF1 using unpurified Anti-COUP TF1 antibody [EPR10841] ab181137 at 1/100. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody, and Hematoxylin for counterstaining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-COUP TF1 antibody [EPR10841] ab181137).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)

    This IHC data was generated using the same anti-COUP TF1 antibody clone [EPR10841] in a different buffer formulation (cat# Anti-COUP TF1 antibody [EPR10841] ab181137).

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma staining COUP TF1 using Anti-COUP TF1 antibody [EPR10841] ab181137 at 1/100. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody, and Hematoxylin for counterstaining.

  • Flow Cytometry (Intracellular) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)

    Intracellular flow cytometric analysis of COUP TF1 labeling 2% paraformaldehyde fixed 293 cells using unpurified Anti-COUP TF1 antibody [EPR10841] ab181137 at 1/70 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) secondary antibody was used at 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-COUP TF1 antibody [EPR10841] ab181137).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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