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Mouse Monoclonal COX IV antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse, Rat, Cow samples. Cited in 329 publications.


Images

Western blot - Anti-COX IV antibody [20E8C12] (AB14744), expandable thumbnail
  • Flow Cytometry - Anti-COX IV antibody [20E8C12] (AB14744), expandable thumbnail
  • Western blot - Anti-COX IV antibody [20E8C12] (AB14744), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.4 - 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline

Form
Liquid
Clonality
Monoclonal

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested
Cow
Expected
Tested
Pig
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
0.5 µg for 106 Cells
Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Pig
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1 µg/mL
Notes

-

Species
Rat
Dilution info
1 µg/mL
Notes

-

Species
Human
Dilution info
1 µg/mL
Notes

-

Species
Cow
Dilution info
1 µg/mL
Notes

-

Predicted
Predicted

Species
Pig
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Alternative names

COX4, COX4I1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1

Recommended products

Mouse Monoclonal COX IV antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse, Rat, Cow samples. Cited in 329 publications.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Clone number
20E8C12
Purity
IgG fraction
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

This antibody makes an effective loading control for mitochondria. COXIV is generally expressed at a consistent high level. However, be aware that many proteins run at the same 16kD size as COXIV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size. Some caution is required when using this antibody as a loading control as COXIV expression can vary under some manipulations. An alternative mitochondrial loading control is Rabbit polyclonal to COX IV antibody (Anti-COX IV antibody - Mitochondrial Loading Control ab16056).

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cytochrome c oxidase subunit IV commonly known as COX IV is a component of the enzyme complex located in the inner mitochondrial membrane. COX IV has a molecular weight of approximately 17 kDa and serves as a subunit of the larger cytochrome c oxidase complex which is essential in cellular respiration. As a mitochondrial marker COX IV is expressed in various tissues where it acts as an important player in the electron transport chain. The presence and function of COX IV are critical in facilitating the last step of the mitochondrial respiratory chain.

Biological function summary

COX IV acts as a significant part of the cytochrome c oxidase complex helping catalyze the reduction of oxygen to water. This process is an important step in the overall mechanism of oxidative phosphorylation. COX IV's role in this complex enables the proton gradient generation across the inner mitochondrial membrane which is necessary for ATP synthesis. Its activity regulates the efficiency of cellular respiration impacting energy production and metabolic activities within cells.

Pathways

COX IV functionally interacts within the oxidative phosphorylation and electron transport chain pathways. Its coordination with other proteins like COX I and COX II in the cytochrome c oxidase complex ensures proper electron transfer to oxygen. Additionally COX IV is implicated in the regulation of reactive oxygen species maintaining cellular homeostasis. These pathways interconnect with broader cellular mechanisms that involve energy metabolism and apoptosis.

Associated diseases and disorders

COX IV has been linked to mitochondrial disorders where defects in the oxidative phosphorylation processes can lead to conditions such as mitochondrial encephalomyopathy. Abnormalities in COX IV function and expression can also contribute to neurodegenerative diseases including Parkinson's disease. Here interactions with proteins like superoxide dismutase (SOD) highlight how oxidative stress and mitochondrial dysfunction relate closely to disease progression. These associations underline the importance of COX IV in maintaining cellular and organismal health.

Product promise

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Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-COX IV antibody [20E8C12] (ab14744), expandable thumbnail

    Western blot - Anti-COX IV antibody [20E8C12] (ab14744)

    This image is courtesy of an Abreview submitted by Camilla Skjerpen on 4 July 2005.

    All lanes: Western blot - Anti-COX IV antibody [20E8C12] (ab14744) at 1/5000 dilution

    Lane 1: Cytoplasmic fraction mouse NIH/3T3 cell lysate

    Lane 2: Nuclear fraction mouse NIH/3T3 cell lysate

    Secondary

    All lanes: HRP conjugated goat anti-mouse antibody

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 19 kDa

    Observed band size: 16 kDa

    Exposure time: 3min

  • Flow Cytometry - Anti-COX IV antibody [20E8C12] (ab14744), expandable thumbnail

    Flow Cytometry - Anti-COX IV antibody [20E8C12] (ab14744)

    Overlay histogram showing HeLa cells stained with ab14744 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14744, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-COX IV antibody [20E8C12] (ab14744), expandable thumbnail

    Western blot - Anti-COX IV antibody [20E8C12] (ab14744)

    All lanes: Western blot - Anti-COX IV antibody [20E8C12] (ab14744) at 1 µg/mL

    Lane 1: Isolated mitochondria from human heart at 5 µg

    Lane 2: Isolated mitochondria from bovine heart at 1 µg

    Lane 3: Isolated mitochondria from rat heart at 10 µg

    Lane 4: Isolated mitochondria from murine heart at 10 µg

    Predicted band size: 19 kDa

    Observed band size: 16 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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