AB14744

Anti-COX IV antibody [20E8C12] - Mitochondrial Marker

Name: Anti-COX IV antibody [20E8C12] - Mitochondrial Marker (ab14744) | Abcam
Brand: Abcam Limited
SKU: AB14744
Price: 400 USD
Availability: InStock
Rating: 5 (23 reviews)

Lab Essentials
What is this?

(23 Reviews)

(365 Publications)

Anti-COX IV antibody [20E8C12] (ab14744) is a mouse monoclonal antibody detecting COX IV in Western Blot, Flow Cytometry. Suitable for Cow, Human, Mouse, Rat.

- Over 330 publications
- Trusted since 2004

View Alternative Names

COX4, COX4I1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1

3 Images

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Western blot - Anti-COX IV antibody [20E8C12] - Mitochondrial Marker (AB14744)

All lanes:

Western blot - Anti-COX IV antibody [20E8C12] - Mitochondrial Marker (ab14744) at 1 µg/mL

Lane 1:

Isolated mitochondria from human heart at 5 µg

Lane 2:

Isolated mitochondria from bovine heart at 1 µg

Lane 3:

Isolated mitochondria from rat heart at 10 µg

Lane 4:

Isolated mitochondria from murine heart at 10 µg

Predicted band size: 19 kDa

Observed band size: 16 kDa

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Flow Cytometry - Anti-COX IV antibody [20E8C12] - Mitochondrial Marker (AB14744)

Flow Cyt

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Flow Cytometry - Anti-COX IV antibody [20E8C12] - Mitochondrial Marker (AB14744)

Overlay histogram showing HeLa cells stained with ab14744 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14744, 0.5μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

AbReview2993****

Western blot - Anti-COX IV antibody [20E8C12] - Mitochondrial Marker (AB14744)

This image is courtesy of an Abreview submitted by Camilla Skjerpen on 4 July 2005.

All lanes:

Western blot - Anti-COX IV antibody [20E8C12] - Mitochondrial Marker (ab14744) at 1/5000 dilution

Lane 1:

Cytoplasmic fraction mouse NIH/3T3 cell lysate

Lane 2:

Nuclear fraction mouse NIH/3T3 cell lysate

Secondary

All lanes:

HRP conjugated goat anti-mouse antibody

Predicted band size: 19 kDa

Observed band size: 16 kDa

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Exposure time: 3min

HRP

HRP Anti-COX IV antibody [20E8C12] - Mitochondrial Marker

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

20E8C12

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat, Cow, Human

Applications

Flow Cyt, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-COX IV antibody [20E8C12] (ab14744) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt) in Cow, Human, Mouse, Rat samples.

Trusted by the scientific community
Anti-COX IV [20E8C12] (ab14744) was first used in a scientific publication in 2004 and has been cited over 330 times in peer-reviewed journals.

Reviewed by scientists
Anti-COX IV [20E8C12] (ab14744) has over 20 independent reviews from customers.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Storage buffer

pH: 7.4 - 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome c oxidase subunit IV commonly known as COX IV is a component of the enzyme complex located in the inner mitochondrial membrane. COX IV has a molecular weight of approximately 17 kDa and serves as a subunit of the larger cytochrome c oxidase complex which is essential in cellular respiration. As a mitochondrial marker COX IV is expressed in various tissues where it acts as an important player in the electron transport chain. The presence and function of COX IV are critical in facilitating the last step of the mitochondrial respiratory chain.

Biological function summary

COX IV acts as a significant part of the cytochrome c oxidase complex helping catalyze the reduction of oxygen to water. This process is an important step in the overall mechanism of oxidative phosphorylation. COX IV's role in this complex enables the proton gradient generation across the inner mitochondrial membrane which is necessary for ATP synthesis. Its activity regulates the efficiency of cellular respiration impacting energy production and metabolic activities within cells.

Pathways

COX IV functionally interacts within the oxidative phosphorylation and electron transport chain pathways. Its coordination with other proteins like COX I and COX II in the cytochrome c oxidase complex ensures proper electron transfer to oxygen. Additionally COX IV is implicated in the regulation of reactive oxygen species maintaining cellular homeostasis. These pathways interconnect with broader cellular mechanisms that involve energy metabolism and apoptosis.

COX IV has been linked to mitochondrial disorders where defects in the oxidative phosphorylation processes can lead to conditions such as mitochondrial encephalomyopathy. Abnormalities in COX IV function and expression can also contribute to neurodegenerative diseases including Parkinson's disease. Here interactions with proteins like superoxide dismutase (SOD) highlight how oxidative stress and mitochondrial dysfunction relate closely to disease progression. These associations underline the importance of COX IV in maintaining cellular and organismal health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

See full target information COX4I1

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Publications (365)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5261 PubMed40480980

2025

TANGO2 binds crystallin alpha B and its loss causes desminopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Maike Stentenbach,Laetitia A Hughes,Samuel V Fagan,Blake Payne,Danielle L Rudler,Stefan J Siira,Tim McCubbin,Anaëlle Chopin,Kara L Perks,Judith A Ermer,James Hendry,Teagan S Er,Shanti Balasubramaniam,Joel A Eliades,Livia C Hool,Nicolle H Packer,Edward S X Moh,Benjamin S Padman,Oliver Rackham,Aleksandra Filipovska

Frontiers in neurology 16:1533092 PubMed40260133

2025

Acupuncture alleviates hemorrhagic transformation after delayed rt-PA treatment for acute ischemic stroke by regulating the mitophagy-NLRP3 inflammasome pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Jiang,Qianqian Liu,Huanhuan Liu,Zheng Huang,Mengning Yang,Peiyan Huang,Yiting Shen,Yangyang Song,Wentao Xu,Xinchang Zhang,Guangxia Ni

Translational exercise biomedicine 2:9-20 PubMed40224168

2025

Mitochondrial and cardiovascular responses to aerobic exercise training in supine and upright positions in healthy young adults: a randomized parallel arm trial.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Preobrazenski,Stuart P S Mladen,Ejaz Causer,Eveline Menezes,Hashim Islam,Patrick J Drouin,Michael E Tschakovsky,Brendon J Gurd

Molecular metabolism 96:102134 PubMed40189102

2025

C3aR1 on β cells enhances β cell function and survival to maintain glucose homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Renan Pereira de Lima,Ang Li,Ankit Gilani,Alfonso Rubio-Navarro,Charles D Warren,Isabella Y Kong,Jacob B Geri,James C Lo

Science advances 11:eadu3011 PubMed39983002

2025

Mitochondrial translation regulates terminal erythroid differentiation by maintaining iron homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuya Morishima,Md Fakruddin,Yohei Kanamori,Takeshi Masuda,Akiko Ogawa,Yuxin Wang,Vivien A C Schoonenberg,Falk Butter,Yuichiro Arima,Takaaki Akaike,Toshiro Moroishi,Kazuhito Tomizawa,Toshio Suda,Fan-Yan Wei,Hitoshi Takizawa

International journal of molecular sciences 26: PubMed39940884

2025

Adult Leigh Syndrome Associated with the m.15635T>C Mitochondrial DNA Variant Affecting the Cytochrome () Gene.

Applications

Unspecified application

Species

Unspecified reactive species

Concetta Valentina Tropeano,Chiara La Morgia,Alessandro Achilli,Luisa Iommarini,Gaia Tioli,Leonardo Caporali,Anna Olivieri,Maria Lucia Valentino,Rocco Liguori,Piero Barboni,Andrea Martinuzzi,Caterina Tonon,Raffaele Lodi,Antonio Torroni,Valerio Carelli,Anna Maria Ghelli

International journal of molecular sciences 26: PubMed39941083

2025

Biotin Induces Inactive Chromosome X Reactivation and Corrects Physiopathological Alterations in Beta-Propeller-Protein-Associated Neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Reche-López,Ana Romero-González,Mónica Álvarez-Córdoba,Alejandra Suárez-Carrillo,Paula Cilleros-Holgado,Rocío Piñero-Pérez,David Gómez-Fernández,José Manuel Romero-Domínguez,Alejandra López-Cabrera,Susana González-Granero,José Manuel García-Verdugo,José A Sánchez-Alcázar

Life science alliance 8: PubMed39779219

2025

COA5 has an essential role in the early stage of mitochondrial complex IV assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Xin Tang,Alfredo Cabrera-Orefice,Jana Meisterknecht,Lucie S Taylor,Geoffray Monteuuis,Maria Ekman Stensland,Adam Szczepanek,Karen Stals,James Davison,Langping He,Sila Hopton,Tuula A Nyman,Christopher B Jackson,Angela Pyle,Monika Winter,Ilka Wittig,Robert W Taylor

The Journal of biological chemistry 300:107728 PubMed39214298

2024

Defective post-transcriptional modification of tRNA disrupts mitochondrial homeostasis in Leber's hereditary optic neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Juanjuan Zhang,Wenxu Li,Zhen Liu,Yingqi Chen,Xiaoyang Wei,Lu Peng,Man Xu,Yanchun Ji

iScience 27:110560 PubMed39184436

2024

Mitochondrial translation is the primary determinant of secondary mitochondrial complex I deficiencies.

Applications

Unspecified application

Species

Unspecified reactive species

Kristýna Čunátová,Marek Vrbacký,Guillermo Puertas-Frias,Lukáš Alán,Marie Vanišová,María José Saucedo-Rodríguez,Josef Houštěk,Erika Fernández-Vizarra,Jiří Neužil,Alena Pecinová,Petr Pecina,Tomáš Mráček

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-COX IV antibody [20E8C12] - Mitochondrial Marker

Western blot - Anti-COX IV antibody [20E8C12] - Mitochondrial Marker (AB14744)

Flow Cytometry - Anti-COX IV antibody [20E8C12] - Mitochondrial Marker (AB14744)

Western blot - Anti-COX IV antibody [20E8C12] - Mitochondrial Marker (AB14744)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (365)

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