Rabbit Polyclonal COX IV antibody. Suitable for WB, IHC-P, IHC - Wmt and reacts with Zebrafish, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Cytochrome c oxidase subunit 4 isoform 1, mitochondrial aa 1 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
WB | IHC-P | IHC - Wmt | |
---|---|---|---|
Human | Expected | Expected | Expected |
Zebrafish | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Zebrafish | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Zebrafish | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Zebrafish | Dilution info 1/100 - 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
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Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
COX4, COX4I1, COX4, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1
Rabbit Polyclonal COX IV antibody. Suitable for WB, IHC-P, IHC - Wmt and reacts with Zebrafish, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Cytochrome c oxidase subunit 4 isoform 1, mitochondrial aa 1 to C-terminus.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Store undiluted
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This supplementary information is collated from multiple sources and compiled automatically.
Cytochrome c oxidase subunit IV commonly known as COX IV is a component of the enzyme complex located in the inner mitochondrial membrane. COX IV has a molecular weight of approximately 17 kDa and serves as a subunit of the larger cytochrome c oxidase complex which is essential in cellular respiration. As a mitochondrial marker COX IV is expressed in various tissues where it acts as an important player in the electron transport chain. The presence and function of COX IV are critical in facilitating the last step of the mitochondrial respiratory chain.
COX IV acts as a significant part of the cytochrome c oxidase complex helping catalyze the reduction of oxygen to water. This process is an important step in the overall mechanism of oxidative phosphorylation. COX IV's role in this complex enables the proton gradient generation across the inner mitochondrial membrane which is necessary for ATP synthesis. Its activity regulates the efficiency of cellular respiration impacting energy production and metabolic activities within cells.
COX IV functionally interacts within the oxidative phosphorylation and electron transport chain pathways. Its coordination with other proteins like COX I and COX II in the cytochrome c oxidase complex ensures proper electron transfer to oxygen. Additionally COX IV is implicated in the regulation of reactive oxygen species maintaining cellular homeostasis. These pathways interconnect with broader cellular mechanisms that involve energy metabolism and apoptosis.
COX IV has been linked to mitochondrial disorders where defects in the oxidative phosphorylation processes can lead to conditions such as mitochondrial encephalomyopathy. Abnormalities in COX IV function and expression can also contribute to neurodegenerative diseases including Parkinson's disease. Here interactions with proteins like superoxide dismutase (SOD) highlight how oxidative stress and mitochondrial dysfunction relate closely to disease progression. These associations underline the importance of COX IV in maintaining cellular and organismal health.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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All lanes: Western blot - Anti-COX IV antibody (ab209727) at 1/1000 dilution
All lanes: adult Zebrafish lysate at 10 µg
Predicted band size: 19 kDa
Immunohistochemical analysis of formalin fixed, paraffin embedded Zebrafish tissue labeling COX IV using ab209727 at 1/300 dilution.
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