Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free - Mitochondrial Marker

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free - Mitochondrial Marker (AB231168)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling COX IV with ab202554 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Cytoplasmic staining on HepG2 cells is observed.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :
-ve control 1 : ab202554 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202554).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free - Mitochondrial Marker (AB231168)

This IHC data was generated using the same anti-COX IV antibody clone, EPR9442(ABC), in a different buffer formulation (cat# ab202554).

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling COX IV with ab202554 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasmic staining on Human hepatocellular carcinoma tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free - Mitochondrial Marker (AB231168)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling COX IV with ab202554 at 1/20 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202554).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free - Mitochondrial Marker (AB231168)

This ICC data was generated using the same anti-COX IV antibody clone, EPR9442(ABC), in a different buffer formulation (cat# ab202554).

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling COX IV with ab202554 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Cytoplasmic staining on HeLa cells is observed.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :
-ve control 1 : ab202554 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free - Mitochondrial Marker (AB231168)

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling COX IV with ab202554 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasmic staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202554).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free - Mitochondrial Marker (AB231168)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human breast carcinoma tissue labeling BCAT2 with ab309514 at 1/5000 Panel A : merged staining of anti-BCAT2 (green; Opal™520) and anti-COX IV (red; Opal™570) on human breast carcinoma. Panel B : anti-BCAT2 stained on mitochondria. Panel C : anti-COX IV stained on mitochondria. The section was incubated in two rounds of staining : in the order of ab309514 and ab231168 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Counterstained with DAPI. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free - Mitochondrial Marker (AB231168)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202554).

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human cardiac muscle staining of GPT2 with ab322261 at a 1/2000 (0.261 µg/ml) dilution and COX IV with ab202554 at 1/500 (0.228 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-GPT2 (green; Opal™570) and anti-COX IV (magenta; Opal™520) on human cardiac muscle.
Panel B : anti-GPT2 showed granular staining in human cardiac muscle.
Panel C : ant-COX IV showed granular staining in human cardiac muscle.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322261 and ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free - Mitochondrial Marker (AB231168)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202554).

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human kidney staining of GPT2 with ab322261 at a 1/2000 (0.261 µg/ml) dilution and COX IV with ab202554 at 1/500 (0.228 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-GPT2 (green; Opal™570) and anti-COX IV (magenta; Opal™520) on human kidney.
Panel B : anti-GPT2 showed granular staining in human kidney.
Panel C : ant-COX IV showed granular staining in human kidney.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322261 and ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free - Mitochondrial Marker (AB231168)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human endometrium tissue labeling BCAT2 with ab309514 at 1/5000 Panel A : merged staining of anti-BCAT2 (green; Opal™520) and anti-COX IV (red; Opal™570) on human endometrium. Panel B : anti-BCAT2 stained on mitochondria. Panel C : anti-COX IV stained on mitochondria. The section was incubated in two rounds of staining : in the order of ab309514 and ab231168 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Counterstained with DAPI. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free - Mitochondrial Marker (AB231168)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling COX IV with ab202554 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasmic staining on Rat cardiac muscle tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202554).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free - Mitochondrial Marker (AB231168)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling COX IV with ab202554 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasmic staining on mouse kidney tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202554).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free - Mitochondrial Marker (AB231168)

COX IV was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab202554 at 1/20 dilution.

Western blot was performed from the immunoprecipitate using ab202554 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

Lane 1 : Human fetal heart whole cell lysate 10 μg (Input).

Lane 2 : ab202554 IP in Human fetal heart whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab202554 in Human fetal heart whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202554).

All lanes:

Immunoprecipitation - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (<a href='/en-us/products/primary-antibodies/cox-iv-antibody-epr9442abc-mitochondrial-loading-control-ab202554'>ab202554</a>)

Predicted band size: 19 kDa,22 kDa

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