Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human colon tissue staining PDHA1 (phospho S300) with ab324265 at a 1 : 500 (0.994 ug/ml) dilution, ab202554 anti-COX4 used at 1 : 500 (0.228 ug/ml) dilution.

Panel A : merged staining of anti-PDHA1 (phospho S300) (green; Opal™520), anti-COX4 (magenta; Opal™570) on human colon.
Panel B : anti-PDHA1 (phospho S300) showed granular staining in human colon.
Panel C : anti-COX4 showed granular staining in human colon.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324265, ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling COX IV with ab202554 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Cytoplasmic staining on HepG2 cells is observed.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :
-ve control 1 : ab202554 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling COX IV with ab202554 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasmic staining on Human hepatocellular carcinoma tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling COX IVwith ab202554 at 1/20 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730;black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling COX IV with ab202554 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Cytoplasmic staining on HeLa cells is observed.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :
-ve control 1 : ab202554 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling COX IV with ab202554 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasmic staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining Adenine Nucleotide Translocator 2/ANT 2 with ab325120 at a 1 : 100 (5.04 ug/ml) dilution, ab202554 anti-COX IV used at 1 : 500 (0.23 ug/ml) dilution.

Panel A : merged staining of anti-ANT 2 (green; Opal™520) and anti-COX IV (magenta; Opal™570) on human liver.
Panel B : anti-ANT 2 showed mitochondrial staining in human liver.
Panel C : anti-COX IV showed mitochondrial staining in human liver.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325120 and ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining MARC1 with ab317262 at a 1 : 1000 (0.503 ug/ml) dilution, ab202554 anti-COX IV used at 1 : 500 (0.23 ug/ml) dilution.

Panel A : merged staining of anti-MARC1 (green; Opal™520), anti-COX IV (magenta; Opal™570) on human kidney.
Panel B : anti-MARC1 staining on several kidney tubules of human kidney.
Panel C : anti-COX IV staining on kidney tubules of human kidney.
Panel D : nuclear DNA was labeled with DAPI (shown in blue).
Co-staining of MARC1 and COX IV (mitochondrial marker) can be observed.
The section was incubated in two rounds of staining : in the order of ab317262, ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining MARC1 with ab317262 at a 1 : 1000 (0.503 ug/ml) dilution, ab202554 anti-COX IV used at 1 : 500 (0.23 ug/ml) dilution.

Panel A : merged staining of anti-MARC1 (green; Opal™520), anti-COX IV (magenta; Opal™570) on human liver.
Panel B : anti-MARC1 staining in hepatocyte of human liver.
Panel C : anti-COX IV staining in hepatocyte of human liver.
Panel D : nuclear DNA was labeled with DAPI (shown in blue).
Co-staining of MARC1 and COX IV (mitochondrial marker) can be observed.
The section was incubated in two rounds of staining : in the order of ab317262, ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human colon tissue staining MARC1 with ab317262 at a 1 : 1000 (0.503 ug/ml) dilution, ab202554 anti-COX IV used at 1 : 500 (0.23 ug/ml) dilution.

Panel A : merged staining of anti-MARC1 (green; Opal™520), anti-COX IV (magenta; Opal™570) on human colon.
Panel B : anti-MARC1 staining on human colon.
Panel C : anti-COX IV staining on human colon.
Panel D : nuclear DNA was labeled with DAPI (shown in blue).
Co-staining of MARC1 and COX IV (mitochondrial marker) can be observed.
The section was incubated in two rounds of staining : in the order of ab317262, ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human cardiac muscle staining of GPT2 with ab322261 at a 1/2000 (0.261 µg/ml) dilution and COX IV with ab202554 at 1/500 (0.228 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-GPT2 (green; Opal™570) and anti-COX IV (magenta; Opal™520) on human cardiac muscle.
Panel B : anti-GPT2 showed granular staining in human cardiac muscle.
Panel C : ant-COX IV showed granular staining in human cardiac muscle.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322261 and ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human kidney staining of GPT2 with ab322261 at a 1/2000 (0.261 µg/ml) dilution and COX IV with ab202554 at 1/500 (0.228 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-GPT2 (green; Opal™570) and anti-COX IV (magenta; Opal™520) on human kidney.
Panel B : anti-GPT2 showed granular staining in human kidney.
Panel C : ant-COX IV showed granular staining in human kidney.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322261 and ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining Cytochrome P450 2E1 with ab324160 at a 1 : 2000 (0.262 ug/ml) dilution, ab202554 anti-COX IV used at 1 : 500 (0.228 ug/ml) dilution.

Panel A : merged staining of anti-CYP2E1 (green; Opal™520) and anti-COX IV (magenta; Opal™690) on human liver.
Panel B : anti-CYP2E1 staining hepatocytes in human liver.
Panel C : ant-COX IV staining hepatocytes in human liver.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324160 and ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining MT-ND4 with ab324743 at a 1 : 100 (5.2 ug/ml) dilution, ab202554 anti-COX4I1 used at 1 : 500 (0.228 ug/ml) dilution.

Panel A : merged staining of anti-MT-ND4 (green; Opal™520), anti-COX4I1 (magenta; Opal™570) on human kidney.
Panel B : anti-MT-ND4 showed mitochondrial staining in human kidney.
Panel C : anti-COX4I1 showed mitochondrial staining in human kidney.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324743 and ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

COX IV was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab202554 at 1/20 dilution.

Western blot was performed from the immunoprecipitate using ab202554 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

Lane 1 : Human fetal heart whole cell lysate 10 μg (Input).

Lane 2 : ab202554 IP in Human fetal heart whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab202554 in Human fetal heart whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)

Predicted band size: 19 kDa,22 kDa

false

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining Cytochrome P450 2E1 with ab324160 at a 1 : 2000 (0.262 ug/ml) dilution, ab202554 anti-COX IV used at 1 : 500 (0.228 ug/ml) dilution.

Panel A : merged staining of anti-CYP2E1 (green; Opal™520) and anti-COX IV (magenta; Opal™690) on mouse liver.
Panel B : anti-CYP2E1 staining hepatocytes in mouse liver.
Panel C : ant-COX IV staining hepatocytes in mouse liver.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324160 and ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining Cytochrome P450 2E1 with ab324160 at a 1 : 2000 (0.262 ug/ml) dilution, ab202554 anti-COX IV used at 1 : 500 (0.228 ug/ml) dilution.

Panel A : merged staining of anti-CYP2E1 (green; Opal™520) and anti-COX IV (magenta; Opal™690) on rat liver.
Panel B : anti-CYP2E1 staining hepatocytes in rat liver.
Panel C : ant-COX IV staining hepatocytes in rat liver.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324160 and ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling COX IV with ab202554 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasmic staining on Rat cardiac muscle tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling COX IV with ab202554 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasmic staining on mouse kidney tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse kidney tissue staining Adenine Nucleotide Translocator 2/ANT 2 with ab325120 at a 1 : 100 (5.04 ug/ml) dilution, ab202554 anti-COX IV used at 1 : 500 (0.23 ug/ml) dilution.

Panel A : merged staining of anti-ANT 2 (green; Opal™520) and anti-COX IV (magenta; Opal™570) on mouse kidney.
Panel B : anti-ANT 2 showed mitochondrial staining in mouse kidney.
Panel C : anti-COX IV showed mitochondrial staining in mouse kidney.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325120 and ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-COX IV antibody [EPR9442 (ABC)] - Mitochondrial Loading Control - (ab202554) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-PDHA1 (phospho S232) antibody [EPR29825-102] (<a href='/en-us/products/primary-antibodies/pdha1-phospho-s232-antibody-epr29825-102-ab325169'>ab325169</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) mitochondria fraction at 20 µg

Lane 2:

HeLa non-mitochondrial fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 43 kDa,17 kDa,36 kDa

false

Exposure time: 26s

• WB

Lab

Western blot - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-COX IV antibody [EPR9442 (ABC)] - Mitochondrial Loading Control (ab202554) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-OMA1 antibody [EPR29198-78] (<a href='/en-us/products/primary-antibodies/oma1-antibody-epr29198-78-ab324586'>ab324586</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) mitochondria fraction at 20 µg

Lane 2:

HeLa non-mitochondrial fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 36 kDa,16 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

In Western blot, Anti-Alpha-synuclein antibody [EPR20535] (ab212184) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] (<a href='/en-us/products/primary-antibodies/total-oxphos-antibody-cocktail-antibody-rm1134-ab317270'>ab317270</a>) at 1/1000 dilution

Lane 1:

MCF7 non-mitochondrial fraction at 20 µg

Lane 2:

MCF7 mitochondria fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 18 kDa,22 kDa,29 kDa,48 kDa,54 kDa,36 kDa,17 kDa

false

Exposure time: 8s

• WB

Supplier Data

Western blot - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554) at 1/2000 dilution

Lane 1:

Human fetal heart lysate at 20 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 19 kDa

Observed band size: 17 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554) at 1/10000 dilution

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Rat heart lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 19 kDa

Observed band size: 17 kDa

false

Exposure time: 3min