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Rabbit Recombinant Monoclonal COX IV antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 35 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Tested
Rat
Expected
Tested
Expected
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/20

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/2000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Species

Rat

Dilution info

1/500

Notes

-

Species

Human

Dilution info

1/500

Notes

-

Target data

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunbit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Alternative names

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Rabbit Recombinant Monoclonal COX IV antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 35 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EPR9442(ABC)

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

  • Western blot - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554), expandable thumbnail

    Western blot - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554) at 1/2000 dilution

    Lane 1: Human fetal heart lysate at 20 µg

    Lane 2: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa

    Observed band size: 17 kDa

    Exposure time: 3min

  • Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling COX IV with ab202554 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Cytoplasmic staining on HepG2 cells is observed.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202554 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling COX IV with ab202554 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Cytoplasmic staining on HeLa cells is observed.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202554 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Western blot - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554), expandable thumbnail

    Western blot - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554) at 1/10000 dilution

    Lane 1: Mouse heart lysate at 10 µg

    Lane 2: Rat heart lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa

    Observed band size: 17 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling COX IV with ab202554 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Cytoplasmic staining on Human hepatocellular carcinoma tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling COX IV with ab202554 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Cytoplasmic staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554), expandable thumbnail

    Immunoprecipitation - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)

    COX IV was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab202554 at 1/20 dilution.

    Western blot was performed from the immunoprecipitate using ab202554 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

    Lane 1: Human fetal heart whole cell lysate 10 μg (Input).

    Lane 2: ab202554 IP in Human fetal heart whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202554 in Human fetal heart whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (AB202554)

    Predicted band size: 19 kDa, 22 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling COX IV with ab202554 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Cytoplasmic staining on mouse kidney tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)

    Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling COX IV with ab202554 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Cytoplasmic staining on Human cervix carcinoma tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling COX IVwith ab202554 at 1/20 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730;black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554), expandable thumbnail

    Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human colon tissue staining MARC1 with ab317262 at a 1:1000 (0.503 ug/ml) dilution, ab202554 anti-COX IV used at 1:500 (0.23 ug/ml) dilution.

    Panel A: merged staining of anti-MARC1 (green; Opal™520), anti-COX IV (magenta; Opal™570) on human colon.

    Panel B: anti-MARC1 staining on human colon.

    Panel C: anti-COX IV staining on human colon.

    Panel D: nuclear DNA was labeled with DAPI (shown in blue).

    Co-staining of MARC1 and COX IV (mitochondrial marker) can be observed.

    The section was incubated in two rounds of staining: in the order of ab317262, ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554), expandable thumbnail

    Western blot - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)

    In Western blot, Anti-Alpha-synuclein antibody [EPR20535] (ab212184) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-Total OXPHOS Antibody Cocktail antibody [RM1134] (AB317270) at 1/1000 dilution

    Lane 1: MCF7 non-mitochondrial fraction at 20 µg

    Lane 2: MCF7 mitochondria fraction at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/100000 dilution

    Observed band size: 18 kDa, 22 kDa, 29 kDa, 48 kDa, 54 kDa, 36 kDa, 17 kDa

    Exposure time: 8s

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-COX IV antibody [EPR9442 (ABC)] - Mitochondrial Loading Control (ab202554) staining at 17kDa dilution.

  • Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554), expandable thumbnail

    Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining MARC1 with ab317262 at a 1:1000 (0.503 ug/ml) dilution, ab202554 anti-COX IV used at 1:500 (0.23 ug/ml) dilution.

    Panel A: merged staining of anti-MARC1 (green; Opal™520), anti-COX IV (magenta; Opal™570) on human liver.

    Panel B: anti-MARC1 staining in hepatocyte of human liver.

    Panel C: anti-COX IV staining in hepatocyte of human liver.

    Panel D: nuclear DNA was labeled with DAPI (shown in blue).

    Co-staining of MARC1 and COX IV (mitochondrial marker) can be observed.

    The section was incubated in two rounds of staining: in the order of ab317262, ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554), expandable thumbnail

    Multiplex immunohistochemistry - Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining MARC1 with ab317262 at a 1:1000 (0.503 ug/ml) dilution, ab202554 anti-COX IV used at 1:500 (0.23 ug/ml) dilution.

    Panel A: merged staining of anti-MARC1 (green; Opal™520), anti-COX IV (magenta; Opal™570) on human kidney.

    Panel B: anti-MARC1 staining on several kidney tubules of human kidney.

    Panel C: anti-COX IV staining on kidney tubules of human kidney.

    Panel D: nuclear DNA was labeled with DAPI (shown in blue).

    Co-staining of MARC1 and COX IV (mitochondrial marker) can be observed.

    The section was incubated in two rounds of staining: in the order of ab317262, ab202554 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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