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Mouse Monoclonal COX IV antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Mouse, Xenopus laevis, Cow samples.

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Images

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)WBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Predicted
Predicted
Predicted
African green monkey
Predicted
Predicted
Predicted
Chimpanzee
Predicted
Predicted
Predicted
Chinese hamster
Predicted
Predicted
Predicted
Cow
Expected
Tested
Expected
Drosophila C virus
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Xenopus laevis
Expected
Tested
Expected
Zebrafish
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1 µg for 106 Cells

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Xenopus laevis, Cow

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Sheep, Chimpanzee, Monkey, African green monkey, Zebrafish, Drosophila C virus, Chinese hamster

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse, Human, Xenopus laevis, Cow

Dilution info

-

Notes

-

Predicted
Predicted

Species

Rat, Sheep, Chimpanzee, Monkey, African green monkey, Zebrafish, Drosophila C virus, Chinese hamster

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Xenopus laevis, Cow

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Sheep, Chimpanzee, Monkey, African green monkey, Zebrafish, Drosophila C virus, Chinese hamster

Dilution info

-

Notes

-

Target data

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunbit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Alternative names

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Mouse Monoclonal COX IV antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Mouse, Xenopus laevis, Cow samples.

Alternative names

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

mAbcam33985

Purity

IgG fraction

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab237963 is the carrier-free version of ab33985.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

Biological function summary

COX IV acts as a significant part of the cytochrome c oxidase complex helping catalyze the reduction of oxygen to water. This process is an important step in the overall mechanism of oxidative phosphorylation. COX IV's role in this complex enables the proton gradient generation across the inner mitochondrial membrane which is necessary for ATP synthesis. Its activity regulates the efficiency of cellular respiration impacting energy production and metabolic activities within cells.

Activity summary

Cytochrome c oxidase subunit IV commonly known as COX IV is a component of the enzyme complex located in the inner mitochondrial membrane. COX IV has a molecular weight of approximately 17 kDa and serves as a subunit of the larger cytochrome c oxidase complex which is essential in cellular respiration. As a mitochondrial marker COX IV is expressed in various tissues where it acts as an important player in the electron transport chain. The presence and function of COX IV are critical in facilitating the last step of the mitochondrial respiratory chain.

Pathways

COX IV functionally interacts within the oxidative phosphorylation and electron transport chain pathways. Its coordination with other proteins like COX I and COX II in the cytochrome c oxidase complex ensures proper electron transfer to oxygen. Additionally COX IV is implicated in the regulation of reactive oxygen species maintaining cellular homeostasis. These pathways interconnect with broader cellular mechanisms that involve energy metabolism and apoptosis.

Associated diseases and disorders

COX IV has been linked to mitochondrial disorders where defects in the oxidative phosphorylation processes can lead to conditions such as mitochondrial encephalomyopathy. Abnormalities in COX IV function and expression can also contribute to neurodegenerative diseases including Parkinson's disease. Here interactions with proteins like superoxide dismutase (SOD) highlight how oxidative stress and mitochondrial dysfunction relate closely to disease progression. These associations underline the importance of COX IV in maintaining cellular and organismal health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - BSA and Azide free (ab237963), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - BSA and Azide free (ab237963)

    ICC/IF image of ab33985 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33985, 1μg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine, and sodium azide (ab33985).

  • Western blot - Anti-COX IV antibody [mAbcam33985] - BSA and Azide free (ab237963), expandable thumbnail

    Western blot - Anti-COX IV antibody [mAbcam33985] - BSA and Azide free (ab237963)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine, and sodium azide (ab33985).

    All lanes: Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB33985) at 1 µg/mL

    Lane 1: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Lane 2: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 3: Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Lane 4: Skeletal Muscle (Mouse) Tissue Lysate at 10 µg

    Lane 5: Kidney (Cow) Tissue Lysate (ab29073) at 10 µg

    Secondary

    All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 19 kDa

    Observed band size: 15 kDa

    Exposure time: 1min

  • Flow Cytometry (Intracellular) - Anti-COX IV antibody [mAbcam33985] - BSA and Azide free (ab237963), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-COX IV antibody [mAbcam33985] - BSA and Azide free (ab237963)

    Overlay histogram showing HeLa cells stained with ab33985 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33985, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine, and sodium azide (ab33985).

  • Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - BSA and Azide free (ab237963), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - BSA and Azide free (ab237963)

    Ab33985 staining COX IV in HeLa (Human cervix adenocarcinoma epithelial cell) cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1:1000 dilution (1μg/ml). An Alexa Fluor®594 Goat anti-mouse (ab150120) was used as a secondary antibody at 1:1000 dilution (2 μg/ml). Cells were counterstained with anti-Cyclophilin F (ab231155, 5.5μg/ml) and AlexaFluor®488 Goat anti-Rabbit (ab150077, 2μg/ml). DAPI was used as a nuclear counterstain. Ab33985 was used for negative control 1 at 1:1000 dilution (1μg/ml). For negative control 2, ab231155 was used at a 1:100 dilution (5.5μg/ml) and ab150129 was used as a secondary antibody at 1:1000 dilution (2μg/ml). Confocal image showing mitochondrial staining in HeLa cell line.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine, and sodium azide (ab33985).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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