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Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) is a mouse monoclonal antibody that is used to detect COX IV in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Cow, Human, Mouse, Xenopus laevis samples.



- Over 120 publications

- Trusted since 2007


Images

Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB33985), expandable thumbnail
  • Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB33985), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB33985), expandable thumbnail
  • Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB33985), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB33985), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)WBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Predicted
Predicted
Predicted
African green monkey
Predicted
Predicted
Predicted
Chimpanzee
Predicted
Predicted
Predicted
Chinese hamster
Predicted
Predicted
Predicted
Cow
Expected
Tested
Expected
Drosophila C virus
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Xenopus laevis
Expected
Tested
Expected
Zebrafish
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1 µg for 106 Cells
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Xenopus laevis, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat, Sheep, Chimpanzee, Monkey, Zebrafish, African green monkey, Chinese hamster, Drosophila C virus
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Species
Mouse
Dilution info
1 µg/mL
Notes

-

Species
Xenopus laevis
Dilution info
1 µg/mL
Notes

-

Species
Cow
Dilution info
1 µg/mL
Notes

-

Predicted
Predicted

Species
Rat, Sheep, Chimpanzee, Monkey, Zebrafish, African green monkey, Chinese hamster, Drosophila C virus
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Expected
Expected

Species
Mouse, Xenopus laevis, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat, Sheep, Chimpanzee, Monkey, Zebrafish, African green monkey, Chinese hamster, Drosophila C virus
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Alternative names

Recommended products

Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) is a mouse monoclonal antibody that is used to detect COX IV in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Cow, Human, Mouse, Xenopus laevis samples.



- Over 120 publications

- Trusted since 2007

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
mAbcam33985
Purification technique
Affinity purification Protein G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF), in Cow, Human, Mouse, Xenopus laevis samples.

What is the molecular weight of COX IV?


Anti-COX IV [mAbcam33985] - Mitochondrial Marker (ab33985) specifically detects a band for COX IV (UniProt: P13073) at a molecular weight of 15kDa.

Recommended positive controls


WB: Jurkat and HepG2 whole cell lysates and human skeletal muscle, mouse skeletal muscle and cow kidney tissue lysates. ICC/IF: HeLa cells; Flow Cyt (Intra): HeLa cells.

Trusted by the scientific community


Anti-COX IV [mAbcam33985] - Mitochondrial Marker (ab33985) was first used in a scientific publication in 2007 and has been cited over 120 times in peer-reviewed journals.

Reviewed by scientists


Anti-COX IV [mAbcam33985] - Mitochondrial Marker (ab33985) has over 25 independent reviews from customers.

Other related products


We have a range of other formats of antibody clone [mAbcam33985] also available for your convenience:
ab33985, HRP - HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker ab62164, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker ab197491



Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cytochrome c oxidase subunit IV commonly known as COX IV is a component of the enzyme complex located in the inner mitochondrial membrane. COX IV has a molecular weight of approximately 17 kDa and serves as a subunit of the larger cytochrome c oxidase complex which is essential in cellular respiration. As a mitochondrial marker COX IV is expressed in various tissues where it acts as an important player in the electron transport chain. The presence and function of COX IV are critical in facilitating the last step of the mitochondrial respiratory chain.

Biological function summary

COX IV acts as a significant part of the cytochrome c oxidase complex helping catalyze the reduction of oxygen to water. This process is an important step in the overall mechanism of oxidative phosphorylation. COX IV's role in this complex enables the proton gradient generation across the inner mitochondrial membrane which is necessary for ATP synthesis. Its activity regulates the efficiency of cellular respiration impacting energy production and metabolic activities within cells.

Pathways

COX IV functionally interacts within the oxidative phosphorylation and electron transport chain pathways. Its coordination with other proteins like COX I and COX II in the cytochrome c oxidase complex ensures proper electron transfer to oxygen. Additionally COX IV is implicated in the regulation of reactive oxygen species maintaining cellular homeostasis. These pathways interconnect with broader cellular mechanisms that involve energy metabolism and apoptosis.

Associated diseases and disorders

COX IV has been linked to mitochondrial disorders where defects in the oxidative phosphorylation processes can lead to conditions such as mitochondrial encephalomyopathy. Abnormalities in COX IV function and expression can also contribute to neurodegenerative diseases including Parkinson's disease. Here interactions with proteins like superoxide dismutase (SOD) highlight how oxidative stress and mitochondrial dysfunction relate closely to disease progression. These associations underline the importance of COX IV in maintaining cellular and organismal health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985), expandable thumbnail
    This image is courtesy of a customer review submitted by Dr Anne-Lore Schlaitz

    Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)

    All lanes: Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1/1000 dilution

    Lane 1: Crude extract prepared from Xenopus laevis egg at 15 µg

    Lane 2: Cytosol lysate prepared from Xenopus laevis egg extract at 15 µg

    Lane 3: Total membrane lysate prepared from Xenopus laevis egg extract at 15 µg

    Secondary

    All lanes: HRP conjugated donkey anti-mouse IgG at 1/4000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 19 kDa

    Observed band size: 15 kDa

    Exposure time: 90min

  • Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)

    All lanes: Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1/1000 dilution

    All lanes: cow liver membrane at 15 µg with Milk

    Secondary

    All lanes: Goat Anti-mouse IgG HRP at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 19 kDa

    Exposure time: 17hr

  • Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)

    ICC/IF image of ab33985 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33985, 1μg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985), expandable thumbnail

    Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)

    All lanes: Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1 µg/mL

    Lane 1: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Lane 2: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Lane 3: Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Lane 4: Skeletal Muscle (Mouse) Tissue Lysate at 10 µg

    Lane 5: Kidney (Cow) Tissue Lysate (ab29073) at 10 µg

    Secondary

    All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 19 kDa

    Observed band size: 15 kDa

    Exposure time: 1min

  • Flow Cytometry (Intracellular) - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)

    Overlay histogram showing HeLa cells stained with ab33985 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33985, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)

    ab33985 staining COX IV in human proximal tubular epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 in PBS and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS) for 45 minutes at 20°C. Goat Anti-Mouse IgG H&L (FITC) ab6785, a FITC-conjugated goat anti-mouse IgG (H+L) polyclonal was used as the secondary antibody (1/1000).

  • Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)

    ab33985 staining COX IV in HeLa (Human cervix adenocarcinoma epithelial cell) cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1:1000 dilution (1μg/ml). An Alexa Fluor®594 Goat anti-mouse (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) was used as a secondary antibody at 1:1000 dilution (2 μg/ml). Cells were counterstained with anti-Cyclophilin F (Anti-Cyclophilin F antibody [EPR11311-121] ab231155, 5.5μg/ml) and AlexaFluor®488 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, 2μg/ml). DAPI was used as a nuclear counterstain. ab33985 was used for negative control 1 at 1:1000 dilution (1μg/ml). For negative control 2, Anti-Cyclophilin F antibody [EPR11311-121] ab231155 was used at a 1:100 dilution (5.5μg/ml) and Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 was used as a secondary antibody at 1:1000 dilution (2μg/ml). Confocal image showing mitochondrial staining in HeLa cell line.

  • Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Caco-2 (human colorectal adenocarcinoma epithelial cell) cells labelling HMGCS2 with Anti-HMGCS2 antibody [EPR26978-39] ab305227 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing mitochondrial staining in Caco-2 cells.Negative control: HCT 116 (PMID: 28468827).The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab33985 Anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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