Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker

8 product images

• 

  This image is courtesy of a customer review submitted by Dr Anne-Lore Schlaitz

  Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)

  All lanes: Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1/1000 dilution

  Lane 1: Crude extract prepared from Xenopus laevis egg at 15 µg

  Lane 2: Cytosol lysate prepared from Xenopus laevis egg extract at 15 µg

  Lane 3: Total membrane lysate prepared from Xenopus laevis egg extract at 15 µg

  Secondary

  All lanes: HRP conjugated donkey anti-mouse IgG at 1/4000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 19 kDa

  Observed band size: 15 kDa

  Exposure time: 90min

• 

  This image is courtesy of an anonymous customer review

  Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)

  All lanes: Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1/1000 dilution

  All lanes: cow liver membrane at 15 µg with Milk

  Secondary

  All lanes: Goat Anti-mouse IgG HRP at 1/5000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 19 kDa

  Exposure time: 17hr

• 

  Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)

  ICC/IF image of ab33985 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33985, 1μg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

• 

  Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)

  All lanes: Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1 µg/mL

  Lane 1: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

  Lane 2: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 3: Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

  Lane 4: Skeletal Muscle (Mouse) Tissue Lysate at 10 µg

  Lane 5: Kidney (Cow) Tissue Lysate (ab29073) at 10 µg

  Secondary

  All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 19 kDa

  Observed band size: 15 kDa

  Exposure time: 1min

• 

  Flow Cytometry (Intracellular) - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)

  Overlay histogram showing HeLa cells stained with ab33985 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33985, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

• 

  This image is courtesy of an anonymous customer review

  Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)

  ab33985 staining COX IV in human proximal tubular epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 in PBS and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS) for 45 minutes at 20°C. Goat Anti-Mouse IgG H&L (FITC) ab6785, a FITC-conjugated goat anti-mouse IgG (H+L) polyclonal was used as the secondary antibody (1/1000).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)

  ab33985 staining COX IV in HeLa (Human cervix adenocarcinoma epithelial cell) cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1:1000 dilution (1μg/ml). An Alexa Fluor^®594 Goat anti-mouse (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) was used as a secondary antibody at 1:1000 dilution (2 μg/ml). Cells were counterstained with anti-Cyclophilin F (Anti-Cyclophilin F antibody [EPR11311-121] ab231155, 5.5μg/ml) and AlexaFluor^®488 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, 2μg/ml). DAPI was used as a nuclear counterstain. ab33985 was used for negative control 1 at 1:1000 dilution (1μg/ml). For negative control 2, Anti-Cyclophilin F antibody [EPR11311-121] ab231155 was used at a 1:100 dilution (5.5μg/ml) and Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) ab150129 was used as a secondary antibody at 1:1000 dilution (2μg/ml). Confocal image showing mitochondrial staining in HeLa cell line.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Caco-2 (human colorectal adenocarcinoma epithelial cell) cells labelling HMGCS2 with Anti-HMGCS2 antibody [EPR26978-39] ab305227 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing mitochondrial staining in Caco-2 cells.Negative control: HCT 116 (PMID: 28468827).The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab33985 Anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).
  
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.