Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker

• WB

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Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB33985)

All lanes:

Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1 µg/mL

Lane 1:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 2:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Lane 4:

Skeletal Muscle (Mouse) Tissue Lysate at 10 µg

Lane 5:

Kidney (Cow) Tissue Lysate (<a href='/en-us/products/unavailable/kidney-cow-tissue-lysate-ab29073'>ab29073</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 19 kDa

Observed band size: 15 kDa

true

Exposure time: 1min

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB33985)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labelling EXD2 with ab324454 at 1/50 (9.64 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing mitochondrial staining in PC-3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Low expression : HeLa.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab33985 Anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 (2 ug/ml) dilution (Magenta).

-ve control 1 : ab324454 at 1/50 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution. -ve control 2 : ab33985 at 1/1000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

• ICC/IF

AbReview9117****

Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB33985)

ab33985 staining COX IV in human proximal tubular epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 in PBS and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS) for 45 minutes at 20°C. ab6785, a FITC-conjugated goat anti-mouse IgG (H+L) polyclonal was used as the secondary antibody (1/1000).

This image is courtesy of an anonymous Abreview

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB33985)

ICC/IF image of ab33985 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33985, 1μg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB33985)

ab33985 staining COX IV in HeLa (Human cervix adenocarcinoma epithelial cell) cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1 : 1000 dilution (1μg/ml). An Alexa Fluor^®594 Goat anti-mouse (ab150120) was used as a secondary antibody at 1 : 1000 dilution (2 μg/ml). Cells were counterstained with anti-Cyclophilin F (ab231155, 5.5μg/ml) and AlexaFluor^®488 Goat anti-Rabbit (ab150077, 2μg/ml). DAPI was used as a nuclear counterstain. ab33985 was used for negative control 1 at 1 : 1000 dilution (1μg/ml). For negative control 2, ab231155 was used at a 1 : 100 dilution (5.5μg/ml) and ab150129 was used as a secondary antibody at 1 : 1000 dilution (2μg/ml). Confocal image showing mitochondrial staining in HeLa cell line.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB33985)

Overlay histogram showing HeLa cells stained with ab33985 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33985, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB33985)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Caco-2 (human colorectal adenocarcinoma epithelial cell) cells labelling HMGCS2 with ab305227 at 1/500 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing mitochondrial staining in Caco-2 cells.Negative control : HCT 116 (PMID : 28468827).The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab33985 Anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• WB

AbReview62600****

Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB33985)

All lanes:

Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1/1000 dilution

All lanes:

cow liver membrane at 15 µg

Secondary

All lanes:

Goat Anti-mouse IgG HRP at 1/5000 dilution

Predicted band size: 19 kDa

true

Exposure time: 17hr

This image is courtesy of an anonymous Abreview

• WB

AbReview11842****

Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (AB33985)

All lanes:

Western blot - Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1/1000 dilution

Lane 1:

Crude extract prepared from Xenopus laevis egg at 15 µg

Lane 2:

Cytosol lysate prepared from Xenopus laevis egg extract at 15 µg

Lane 3:

Total membrane lysate prepared from Xenopus laevis egg extract at 15 µg

Secondary

All lanes:

HRP conjugated donkey anti-mouse IgG at 1/4000 dilution

Predicted band size: 19 kDa

Observed band size: 15 kDa

true

Exposure time: 90min

This image is courtesy of an Abreview submitted by Dr Anne-Lore Schlaitz