Anti-COX IV antibody - Mitochondrial Loading Control

11 product images

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  Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)

  ab16056 staining COX IV in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab16056 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).Also suitable in cells fixed with 100% methanol (5 min).
  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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  Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)

  Blocking buffer: 2% BSA
  

  Gel type: MES

  Exposure Time: 1 minute

  All lanes: Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 1 µg/mL

  Lane 1: Human skeletal muscle tissue lysate at 10 µg

  Lane 2: Rat skeletal muscle tissue at 10 µg

  Secondary

  All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

  Predicted band size: 19 kDa

  Observed band size: 17 kDa

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  Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)

  Blocking buffer: 2% BSA
  

  Gel type: MES

  Exposure Time: 1 minute

  All lanes: Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 1 µg/mL

  Lane 1: Human skeletal muscle tissue lysate at 10 µg

  Lane 2: Human heart tissue lysate at 10 µg

  Lane 3: Mouse skeletal muscle tissue lysate at 10 µg

  Secondary

  All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

  Predicted band size: 19 kDa

  Observed band size: 17 kDa

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  Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)

  All lanes: Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 1 µg/mL

  Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg

  Lane 2: Human liver tissue lysate - total protein (ab29889) at 10 µg

  Lane 3: Human heart tissue lysate - total protein (ab29431) at 10 µg

  Lane 4: Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab7090) at 1/3000 dilution

  Performed under reducing conditions.

  Predicted band size: 19 kDa

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  This image is courtesy of an anonymous customer review

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)

  ab16056 staining COX IV in Mouse heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/600 ) for 12 hours at 4°C. A Cy5® donkey anti-rabbit secondary antibody was used as the secondary antibody.

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  Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)

  All lanes: Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 0.38 µg/mL

  Lane 1: Hela whole cell lysate at 20 µg

  Lane 2: Human skeletal muscle cell lysate at 20 µg

  Lane 3: Hela whole cell lysate at 20 µg with Human COX IV peptide (ab16381)

  Lane 4: Human skeletal muscle cell lysate at 20 µg with Human COX IV peptide (ab16381)

  Secondary

  All lanes: HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution

  Predicted band size: 19 kDa

  Observed band size: 15 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)

  IHC image of COXIV staining in human liver FFPE section, performed on a Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16056, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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  This image is courtesy of an anonymous customer review.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)

  ab16056 staining COX IV in breast tumour tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% buffered formalin and blocked with 5% serum for 60 minutes at 21°C; antigen retrieval was by heat mediation in a 10mM sodium citrate buffer pH6. Samples were incubated with primary antibody (1/300 in blocking buffer) for 12 hours at 4°C. An Alexa Fluor^® 647-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

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  This image is courtesy of an anonymous customer review

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)

  Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed human brain tissue. Stained with ab16056 at 1/150 dilution. Secondary antibody used was EnVision+ System- HRP Labelled Polymer anti rabbit. Blocking was done with 5% serum for 1 hour at 21°C. The sample was incubated with the primary antibody and 5% normal goat serum for 19 hours at 4°C. Antigen retrieval method was heat mediated Citrate pH 6.0

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  This image is courtesy of an anonymous customer review

  Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)

  All lanes: Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 1/3000 dilution

  All lanes: mouse heart whole cell lysate at 20 µg

  Secondary

  All lanes: Goat Anti-rabbit IgG, HRP-linked Antibody at 1/1000 dilution

  Developed using the ECL technique.

  Exposure time: 30s

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  This image is courtesy of an anonymous customer review

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056)

  Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed mouse heart tissue. Stained with ab16056 at 1/500 dilution.
  Secondary antibody used was EnVision+ System- HRP Labelled Polymer anti rabbit. Blocking was done with 5% serum for 1 hour at 21°C. The sample was incubated with the primary antibody and 5% normal goat serum for 19 hours at 4°C. Antigen retrieval method was heat mediated Citrate pH 6.0