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Rabbit Polyclonal COX IV antibody. Suitable for WB, IHC-P, IHC - Wmt and reacts with Zebrafish, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human COX4I1 aa 1 to C-terminus.

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Images

Western blot - Anti-COX IV antibody - Mitochondrial Marker (AB209727), expandable thumbnail
  • IHC - Wholemount - Anti-COX IV antibody (AB209727), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody (AB209727), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human COX4I1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P13073

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC - Wmt
Human
Expected
Expected
Expected
Zebrafish
Tested
Tested
Tested

Tested
Tested

Species
Zebrafish
Dilution info
1/500 - 1/3000
Notes

-

Expected
Expected

Species
Human
Dilution info
1/500 - 1/3000
Notes

-

Tested
Tested

Species
Zebrafish
Dilution info
1/100 - 1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
1/100 - 1/1000
Notes

-

Tested
Tested

Species
Zebrafish
Dilution info
1/100 - 1/500
Notes

-

Expected
Expected

Species
Human
Dilution info
1/100 - 1/500
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Alternative names

Recommended products

Rabbit Polyclonal COX IV antibody. Suitable for WB, IHC-P, IHC - Wmt and reacts with Zebrafish, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human COX4I1 aa 1 to C-terminus.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Fragment Protein within Human COX4I1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P13073
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store undiluted

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cytochrome c oxidase subunit IV commonly known as COX IV is a component of the enzyme complex located in the inner mitochondrial membrane. COX IV has a molecular weight of approximately 17 kDa and serves as a subunit of the larger cytochrome c oxidase complex which is essential in cellular respiration. As a mitochondrial marker COX IV is expressed in various tissues where it acts as an important player in the electron transport chain. The presence and function of COX IV are critical in facilitating the last step of the mitochondrial respiratory chain.

Biological function summary

COX IV acts as a significant part of the cytochrome c oxidase complex helping catalyze the reduction of oxygen to water. This process is an important step in the overall mechanism of oxidative phosphorylation. COX IV's role in this complex enables the proton gradient generation across the inner mitochondrial membrane which is necessary for ATP synthesis. Its activity regulates the efficiency of cellular respiration impacting energy production and metabolic activities within cells.

Pathways

COX IV functionally interacts within the oxidative phosphorylation and electron transport chain pathways. Its coordination with other proteins like COX I and COX II in the cytochrome c oxidase complex ensures proper electron transfer to oxygen. Additionally COX IV is implicated in the regulation of reactive oxygen species maintaining cellular homeostasis. These pathways interconnect with broader cellular mechanisms that involve energy metabolism and apoptosis.

Associated diseases and disorders

COX IV has been linked to mitochondrial disorders where defects in the oxidative phosphorylation processes can lead to conditions such as mitochondrial encephalomyopathy. Abnormalities in COX IV function and expression can also contribute to neurodegenerative diseases including Parkinson's disease. Here interactions with proteins like superoxide dismutase (SOD) highlight how oxidative stress and mitochondrial dysfunction relate closely to disease progression. These associations underline the importance of COX IV in maintaining cellular and organismal health.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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3 product images

  • Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab209727), expandable thumbnail

    Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab209727)

    COX IV Western blot staining of adult Zebrafish lysate using rabbit Anti-COX IV antibody

    All lanes: Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab209727) at 1/1000 dilution

    All lanes: adult Zebrafish lysate at 10 µg

    Predicted band size: 19 kDa

  • IHC - Wholemount - Anti-COX IV antibody (ab209727), expandable thumbnail

    IHC - Wholemount - Anti-COX IV antibody (ab209727)

    Immunohistochemical analysis (whole mount) of Zebrafish embryo, labeling COX IV using ab209727 at 1/200 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody (ab209727), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody (ab209727)

    Immunohistochemical analysis of formalin fixed, paraffin embedded Zebrafish tissue labeling COX IV using ab209727 at 1/300 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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