Rabbit Polyclonal COX IV antibody. Mitochondrion marker. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COX4I1 aa 1 to C-terminus.
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- Nature communications 14:70692023Selective activator of human ClpP triggers cell cycle arrest to inhibit lung squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin-Lin Zhou,Tao Zhang,Yun Xue,Chuan Yue,Yihui Pan,Pengyu Wang,Teng Yang,Meixia Li,Hu Zhou,Kan Ding,Jianhua Gan,Hongbin Ji,Cai-Guang YangPubMed 37923710
- Cell death discovery 8:412022KLF2 up-regulates IRF4/HDAC7 to protect neonatal rats from hypoxic-ischemic brain damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan Wu,Chunlin LiPubMed 35091544
- Molecular medicine reports 22:1420-14282020Role of endoplasmic reticulum oxidase 1α in H9C2 cardiomyocytes following hypoxia/reoxygenation injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLina Lai et. al.PubMed 32626998
- Scientific reports 10:66552020Epigenetics and Mitochondrial Stability in the Metabolic Memory Phenomenon Associated with Continued Progression of Diabetic Retinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRenu A Kowluru et. al.PubMed 32313015
- Experimental and therapeutic medicine 19:3227-32382020PM2.5 induces apoptosis, oxidative stress injury and melanin metabolic disorder in human melanocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanfeng Suo et. al.PubMed 32269607
- Autophagy 15:2142-21622019Clearance of damaged mitochondria via mitophagy is important to the protective effect of ischemic preconditioning in kidneys.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMan J Livingston et. al.PubMed 31066324
- Molecular medicine reports 16:515-5222017Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway.
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 30:1416-242015Exosomes from human mesenchymal stem cells conduct aerobic metabolism in term and preterm newborn infants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsabella Panfoli,Silvia Ravera,Marina Podestà,Claudia Cossu,Laura Santucci,Martina Bartolucci,Maurizio Bruschi,Daniela Calzia,Federica Sabatini,Matteo Bruschettini,Luca Antonio Ramenghi,Olga Romantsik,Danilo Marimpietri,Vito Pistoia,Gianmarco Ghiggeri,Francesco Frassoni,Giovanni CandianoPubMed 26655706
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human COX4I1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P13073
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Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Monkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Alternative names
COX4, COX4I1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1
Recommended products
Rabbit Polyclonal COX IV antibody. Mitochondrion marker. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COX4I1 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human COX4I1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P13073
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytochrome c oxidase subunit IV commonly known as COX IV is a component of the enzyme complex located in the inner mitochondrial membrane. COX IV has a molecular weight of approximately 17 kDa and serves as a subunit of the larger cytochrome c oxidase complex which is essential in cellular respiration. As a mitochondrial marker COX IV is expressed in various tissues where it acts as an important player in the electron transport chain. The presence and function of COX IV are critical in facilitating the last step of the mitochondrial respiratory chain.
- Biological function summary
COX IV acts as a significant part of the cytochrome c oxidase complex helping catalyze the reduction of oxygen to water. This process is an important step in the overall mechanism of oxidative phosphorylation. COX IV's role in this complex enables the proton gradient generation across the inner mitochondrial membrane which is necessary for ATP synthesis. Its activity regulates the efficiency of cellular respiration impacting energy production and metabolic activities within cells.
- Pathways
COX IV functionally interacts within the oxidative phosphorylation and electron transport chain pathways. Its coordination with other proteins like COX I and COX II in the cytochrome c oxidase complex ensures proper electron transfer to oxygen. Additionally COX IV is implicated in the regulation of reactive oxygen species maintaining cellular homeostasis. These pathways interconnect with broader cellular mechanisms that involve energy metabolism and apoptosis.
- Associated diseases and disorders
COX IV has been linked to mitochondrial disorders where defects in the oxidative phosphorylation processes can lead to conditions such as mitochondrial encephalomyopathy. Abnormalities in COX IV function and expression can also contribute to neurodegenerative diseases including Parkinson's disease. Here interactions with proteins like superoxide dismutase (SOD) highlight how oxidative stress and mitochondrial dysfunction relate closely to disease progression. These associations underline the importance of COX IV in maintaining cellular and organismal health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-COX IV antibody - Mitochondrial Marker - Mitochondrial Marker (ab153709)
COX IV Western blot staining of HepG2 whole cell lysate using rabbit Anti-COX IV antibody
12% SDS PAGE
All lanes: Western blot - Anti-COX IV antibody - Mitochondrial Marker - Mitochondrial Marker (ab153709) at 1/1000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 19 kDa
Western blot - Anti-COX IV antibody - Mitochondrial Marker - Mitochondrial Marker (ab153709)
COX IV Western blot staining of Mouse muscle whole tissue lysate using rabbit Anti-COX IV antibody
12% SDS PAGE
All lanes: Western blot - Anti-COX IV antibody - Mitochondrial Marker - Mitochondrial Marker (ab153709) at 1/500 dilution
All lanes: Mouse muscle whole tissue lysate at 50 µg
Predicted band size: 19 kDa
Western blot - Anti-COX IV antibody - Mitochondrial Marker - Mitochondrial Marker (ab153709)
COX IV Western blot staining of Rat muscle whole tissue lysate using rabbit Anti-COX IV antibody
12% SDS PAGE
All lanes: Western blot - Anti-COX IV antibody - Mitochondrial Marker - Mitochondrial Marker (ab153709) at 1/500 dilution
All lanes: Rat muscle whole tissue lysate at 50 µg
Predicted band size: 19 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Marker - Mitochondrial Marker (ab153709)
COX IV Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-COX IV antibody
Immunohistochemical analysis of paraffin embedded HS578T (human) xenograft tissue, labeling COX IV using ab153709 at a 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody - Mitochondrial Marker - Mitochondrial Marker (ab153709)
COX IV Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-COX IV antibody
Immunofluorescence analysis of methanol fixed HeLa cells, labeling COX IV using ab153709 at 1/500 dilution (green) and alpha tubulin filaments (red).
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