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AB153709

Anti-COX IV antibody - Mitochondrial Marker

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(10 Publications)

Rabbit Polyclonal COX IV antibody. Mitochondrion marker. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COX4I1 aa 1 to C-terminus.

View Alternative Names

COX4, COX4I1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1

8 Images
Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody - Mitochondrial Marker (AB153709)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody - Mitochondrial Marker (AB153709)

Immunofluorescence analysis of ice-cold MeOH fixed for 5 mins HeLa cells labelling COX4 protein at mitochondria using ab153709 at a 1/500 dilution (Green). The nuclear counterstain is Hoechst 33342 (Blue). Scale bar= 10 µm.

Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody - Mitochondrial Marker (AB153709)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody - Mitochondrial Marker (AB153709)

Immunofluorescence analysis of ice-cold MeOH fixed for 5 mins HeLa cells labelling COX4 protein at mitochondria using ab153709 at a 1/500 dilution (Green). The nuclear counterstain is Hoechst 33342 (Blue). Scale bar= 10 µm.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Marker (AB153709)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Marker (AB153709)

Paraffin-embedded human endometrial carcinoma tissue stained for COX4 protein at mitochondria with ab153709 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min .

Western blot - Anti-COX IV antibody - Mitochondrial Marker (AB153709)
  • WB

Supplier Data

Western blot - Anti-COX IV antibody - Mitochondrial Marker (AB153709)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709) at 1/1000 dilution

Lane 1:

Jurkat whole cell extracts at 30 µg

Lane 2:

Raji whole cell extracts at 30 µg

Lane 3:

A431 whole cell extracts at 30 µg

Lane 4:

HeLa whole cell extracts at 30 µg

Lane 5:

HepG2 whole cell extracts at 30 µg

Lane 6:

A549 whole cell extracts at 30 µg

Lane 7:

HCT116 whole cell extracts at 30 µg

Lane 8:

MCF-7 whole cell extracts at 30 µg

Lane 9:

NT2D1 whole cell extracts at 30 µg

Lane 10:

U87-MG whole cell extracts at 30 µg

Lane 11:

SKNSH whole cell extracts at 30 µg

Lane 12:

K562 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 19 kDa

false

Western blot - Anti-COX IV antibody - Mitochondrial Marker (AB153709)
  • WB

Supplier Data

Western blot - Anti-COX IV antibody - Mitochondrial Marker (AB153709)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709) at 1/3000 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts at 30 µg

Lane 2:

COX IV shRNA transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 19 kDa

false

Western blot - Anti-COX IV antibody - Mitochondrial Marker (AB153709)
  • WB

Supplier Data

Western blot - Anti-COX IV antibody - Mitochondrial Marker (AB153709)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709) at 1/1000 dilution

Lane 1:

Mouse muscle tissue extracts at 50 µg

Lane 2:

Mouse lung tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 19 kDa

false

Western blot - Anti-COX IV antibody - Mitochondrial Marker (AB153709)
  • WB

Supplier Data

Western blot - Anti-COX IV antibody - Mitochondrial Marker (AB153709)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709) at 1/1000 dilution

Lane 1:

Neuro2A whole cell extracts at 30 µg

Lane 2:

NIH-3T3 whole cell extracts at 30 µg

Lane 3:

Raw264.7 whole cell extracts at 30 µg

Lane 4:

C2C12 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 19 kDa

false

Western blot - Anti-COX IV antibody - Mitochondrial Marker (AB153709)
  • WB

Unknown

Western blot - Anti-COX IV antibody - Mitochondrial Marker (AB153709)

12% SDS PAGE

All lanes:

Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709) at 1/500 dilution

All lanes:

Rat muscle whole tissue lysate at 50 µg

Predicted band size: 19 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human COX4I1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P13073

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome c oxidase subunit IV commonly known as COX IV is a component of the enzyme complex located in the inner mitochondrial membrane. COX IV has a molecular weight of approximately 17 kDa and serves as a subunit of the larger cytochrome c oxidase complex which is essential in cellular respiration. As a mitochondrial marker COX IV is expressed in various tissues where it acts as an important player in the electron transport chain. The presence and function of COX IV are critical in facilitating the last step of the mitochondrial respiratory chain.
Biological function summary

COX IV acts as a significant part of the cytochrome c oxidase complex helping catalyze the reduction of oxygen to water. This process is an important step in the overall mechanism of oxidative phosphorylation. COX IV's role in this complex enables the proton gradient generation across the inner mitochondrial membrane which is necessary for ATP synthesis. Its activity regulates the efficiency of cellular respiration impacting energy production and metabolic activities within cells.

Pathways

COX IV functionally interacts within the oxidative phosphorylation and electron transport chain pathways. Its coordination with other proteins like COX I and COX II in the cytochrome c oxidase complex ensures proper electron transfer to oxygen. Additionally COX IV is implicated in the regulation of reactive oxygen species maintaining cellular homeostasis. These pathways interconnect with broader cellular mechanisms that involve energy metabolism and apoptosis.

COX IV has been linked to mitochondrial disorders where defects in the oxidative phosphorylation processes can lead to conditions such as mitochondrial encephalomyopathy. Abnormalities in COX IV function and expression can also contribute to neurodegenerative diseases including Parkinson's disease. Here interactions with proteins like superoxide dismutase (SOD) highlight how oxidative stress and mitochondrial dysfunction relate closely to disease progression. These associations underline the importance of COX IV in maintaining cellular and organismal health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
See full target information COX4I1
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:7069 PubMed37923710

2023

Selective activator of human ClpP triggers cell cycle arrest to inhibit lung squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Lin Zhou,Tao Zhang,Yun Xue,Chuan Yue,Yihui Pan,Pengyu Wang,Teng Yang,Meixia Li,Hu Zhou,Kan Ding,Jianhua Gan,Hongbin Ji,Cai-Guang Yang

Diabetes 72:520-531 PubMed36563021

2022

Mitochondrial Genome-Encoded Long Noncoding RNA and Mitochondrial Stability in Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jay Kumar,Ghulam Mohammad,Kumari Alka,Renu A Kowluru

Cell death discovery 8:41 PubMed35091544

2022

KLF2 up-regulates IRF4/HDAC7 to protect neonatal rats from hypoxic-ischemic brain damage.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Wu,Chunlin Li

Autophagy 17:4102-4118 PubMed33726618

2021

CREG1 improves the capacity of the skeletal muscle response to exercise endurance via modulation of mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

HaiXu Song,Xiaoxiang Tian,Dan Liu,Meili Liu,Yanxia Liu,Jing Liu,Zhu Mei,Chenghui Yan,Yaling Han

Molecular medicine reports 22:1420-1428 PubMed32626998

2020

Role of endoplasmic reticulum oxidase 1α in H9C2 cardiomyocytes following hypoxia/reoxygenation injury.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Lai,Yue Liu,Yuanyuan Liu,Ni Zhang,Shilu Cao,Xiaojing Zhang,Di Wu

Scientific reports 10:6655 PubMed32313015

2020

Epigenetics and Mitochondrial Stability in the Metabolic Memory Phenomenon Associated with Continued Progression of Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Renu A Kowluru,Ghulam Mohammad

Experimental and therapeutic medicine 19:3227-3238 PubMed32269607

2020

PM2.5 induces apoptosis, oxidative stress injury and melanin metabolic disorder in human melanocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Danfeng Suo,Sanwu Zeng,Junling Zhang,Linghe Meng,Lishuo Weng

Autophagy 15:2142-2162 PubMed31066324

2019

Clearance of damaged mitochondria via mitophagy is important to the protective effect of ischemic preconditioning in kidneys.

Applications

Unspecified application

Species

Unspecified reactive species

Man J Livingston,Jinghong Wang,Jiliang Zhou,Guangyu Wu,Ian G Ganley,Joseph A Hill,Xiao-Ming Yin,Zheng Dong

Molecular medicine reports 16:515-522 PubMed28560462

2017

Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway.

Applications

IHC

Species

Rat

Xiaoying Gao,Yun Liu,Yuying Xie,Ying Wang,Sihua Qi

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 30:1416-24 PubMed26655706

2015

Exosomes from human mesenchymal stem cells conduct aerobic metabolism in term and preterm newborn infants.

Applications

Unspecified application

Species

Unspecified reactive species

Isabella Panfoli,Silvia Ravera,Marina Podestà,Claudia Cossu,Laura Santucci,Martina Bartolucci,Maurizio Bruschi,Daniela Calzia,Federica Sabatini,Matteo Bruschettini,Luca Antonio Ramenghi,Olga Romantsik,Danilo Marimpietri,Vito Pistoia,Gianmarco Ghiggeri,Francesco Frassoni,Giovanni Candiano
View all publications
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